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The LexA–RecA* structure reveals a cryptic lock-and-key mechanism for SOS activation
The bacterial SOS response plays a key role in adaptation to DNA damage, including genomic stress caused by antibiotics. SOS induction begins when...
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Single-molecule imaging of LexA degradation in Escherichia coli elucidates regulatory mechanisms and heterogeneity of the SOS response
The bacterial SOS response represents a paradigm of gene networks controlled by a master transcriptional regulator. Self-cleavage of the SOS...
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A LexA-based yeast two-hybrid system for studying light-switchable interactions of phytochromes with their interacting partners
Phytochromes are a family of photoreceptors in plants that perceive the red (R) and far-red (FR) components of their light environment. Phytochromes...
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Detecting Blue Light-Dependent Protein–Protein Interactions by LexA-Based Yeast Two-Hybrid Assay
The LexA-based yeast two-hybrid system is one of the most powerful techniques used to detect blue light-dependent protein–protein interactions. In...
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Synthetic maize centromeres transmit chromosomes across generations
Centromeres are long, often repetitive regions of genomes that bind kinetochore proteins and ensure normal chromosome segregation. Engineering...
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A small bacteriophage protein determines the hierarchy over co-residential jumbo phage in Bacillus thuringiensis serovar israelensis
Bacillus thuringiensis serovar israelensis is the most widely used biopesticide against insects, including vectors of animal and human diseases....
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Transcription factor ZmDof22 enhances drought tolerance by regulating stomatal movement and antioxidant enzymes activities in maize (Zea mays L.)
Key messageThe
...Dof22 gene encoding a deoxyribonucleic acid binding with one finger in maize, which is associated with its drought tolerance.
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Transcriptional repressor Gal80 recruits corepressor complex Cyc8–Tup1 to structural genes of the Saccharomyces cerevisiae GAL regulon
Under non-inducing conditions (absence of galactose), yeast structural genes of the GAL regulon are repressed by Gal80, preventing interaction of...
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The Q-system: A Versatile Repressible Binary Expression System
Binary expression systems are useful genetic tools for experimentally labeling or manipulating the function of defined cells. The Q-system is a...
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Azithromycin can induce SOS response and horizontal gene transfer of SXT element in Vibrio cholerae
BackgroundThe emergence and spread of drug resistance in Vibrio cholerae are mainly attributed to horizontal gene transfer of mobile genetic...
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The influence of oxygen and oxidative stress on de novo acquisition of antibiotic resistance in E. coli and Lactobacillus lactis
BackgroundBacteria can acquire resistance through DNA mutations in response to exposure to sub-lethal concentrations of antibiotics. According to the...
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Contribution of SOS genes to H2O2-induced apoptosis-like death in Escherichia coli
Hydrogen peroxide (H 2 O 2 ) is a debriding agent that damages the microbial structure and function by generating various reactive oxygen species (ROS). H
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Insights into the presence of multiple RecQ helicases in the ancient cyanobacterium, Nostoc sp. strain PCC7120: bioinformatics and expression analysis approach
Owing to their crucial role in genome maintenance, RecQ helicases are ubiquitous and present across organisms. Though the multiplicity of RecQ...
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DNA recombination and repair in Wolbachia: RecA and related proteins
Wolbachia is an obligate intracellular bacterium that has undergone extensive genomic streamlining in its arthropod and nematode hosts. Because the...
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PsrA Regulator Connects Cell Physiology and Class 1 Integron Integrase Gene Expression Through the Regulation of lexA Gene Expression in Pseudomonas spp.
Pseudomonas aeruginosa , which is a clinically important representative of Pseudomonas spp., has been recognized as causative agent of severe...
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Bacterial One- and Two-Hybrid Assays to Monitor Transmembrane Helix Interactions
In transenvelope multiprotein machines such as bacterial secretion systems, protein–protein interactions not only occur between soluble domains but...
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Detection of Protein–Protein Interactions Utilizing the Split-Ubiquitin Membrane-Based Yeast Two-Hybrid System
Identifying the interactors of a protein is a key step in understanding its possible cellular function(s). Among the various methods that can be used...
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ER Membrane Protein Interactions Using the Split-Ubiquitin System (SUS)
Protein-protein interactions (PPIs) play fundamental roles in all cellular processes. Especially membrane proteins facilitate a range of important...
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Analyzing Protein–Protein Interactions Using the Split-Ubiquitin System
The split-ubiquitin technology was developed over 20 years ago as an alternative to Gal4-based, yeast-two-hybrid methods to identify interacting...
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