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Quantifying the Kinetics of Pilus-Specific Sortase-Catalyzed Crosslinking Using High-Performance Liquid Chromatography
Gram-positive bacteria display pili whose protein components (pilins) are covalently crosslinked by pilus-specific sortase enzymes. These cysteine...
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High-Resolution Liquid Chromatography–Mass Spectrometry for Lipidomics
Liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) is a powerful tool for identification and classification of lipids. Ultra-high...
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Measurements of Ascorbate and Dehydroascorbate in Plants Using High-Performance Liquid Chromatography
The current concepts emphasize the fundamental role of reactive oxygen species (ROS) as signaling molecules that coordinate defense mechanisms, cell...
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Determination and analysis of patulin in apples, hawthorns, and their products by high-performance liquid chromatography
This study aimed to establish a high-performance liquid chromatography (HPLC) method to investigate the residues of patulin in apples, hawthorns, and...
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Fluorescence-Based Analyses of Poly(ADP-Ribose) Length by Gel Electrophoresis, High-Performance Liquid Chromatography, and Capillary Electrophoresis
Poly(ADP-ribose) (PAR) is a homopolymer made of two or more adenosine diphosphate ribose (ADP-ribose) units. The polymer is usually conjugated to...
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High performance liquid chromatography (HPLC) based genetic diversity profiling of chilli germplasm for fruit pungency and phytochemical contents
Chilli peppers are widely consumed for their pungency, as used in flavoring the food and has many pharmaceutical and medicinal properties. Based on...
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Metabolic signatures of hepatolithiasis using ultra-high performance liquid chromatography-tandem mass spectrometry
Background & aimsA metabolomic study of hepatolithiasis has yet to be performed. The purpose of the present study was to characterize the metabolite...
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High-Performance Liquid Chromatography Coupled with Tandem Mass Spectrometry Method for the Identification and Quantification of Lipids in Liposomes
Liposomes are spherical, closed vesicles consisting of at least one lipid bilayer with a water chamber and are widely used to encapsulate bioactive...
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Liquid Chromatography and Capillary Electrophoresis Analysis of 2AA-Labeled O-Glycans
To reveal O-glycan structures in mucins, it is necessary to release covalently bound O-glycans from the polypeptide backbone and derivatize to a form...
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Use of High-Performance Liquid Chromatography-Mass Spectrometry (HPLC-MS) to Quantify Modified Nucleosides
Physiological and chemically induced modifications to nucleosides are common in both DNA and RNA. Physiological forms of these modifications play...
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Measurement of Flower Metabolite Concentrations Using Gas Chromatography–Mass Spectrometry and High-Performance Liquid Chromatography–Mass Spectrometry
Metabolite profiling aiming at quantifying the metabolome of flowers is emerging as a suitable tool to understand the metabolic complexity of these...
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Ultra-high-performance liquid chromatography high-resolution mass spectrometry variants for metabolomics research
Ultra-high-performance liquid chromatography high-resolution mass spectrometry (UHPLC–HRMS) variants currently represent the best tools to tackle the...
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Analysis of Content of 2-Oxoacids in Rat Brain Extracts Using High-Performance Liquid Chromatography
Abstract2-Oxoacids are involved in a number of important metabolic processes and can be used as biomarkers in some human diseases. A new optimized...
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Monosaccharide Analysis After One-Pot Derivatization Followed by Reverse-Phase Liquid Chromatography Separation and UV/Vis Detection
Derivatization of monosaccharides with 1-phenyl-3-methyl-5-pyrazolone (PMP) introduces two chromophores per sugar molecule. Their separation on a...
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Evaluation of stability of (1R,2 S)-(−)-2-methylamino-1-phenyl-1-propanol hydrochloride in plasma and urine samples-inoculated with Escherichia coli using high-performance liquid chromatography (HPLC)
The preservation of drug stability in biological evidence during the processes of collection and storage poses a substantial obstacle to the progress...
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Monitoring of catechin content by high performance liquid chromatography during the development of seed and pods of Saraca asoca (Roxb.) De Willd
Saraca asoca (Roxb.) Willd, is an indigenous medicinal plant of family Caesalpiniaceae. Different parts of this plant including seeds and pods,...
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High-Performance Chromatographic Separation of Cerebrosides
High-performance thin layer chromatography (HPTLC) is a very robust, fast, and inexpensive technique that enables separation of complex mixtures....
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Chromatography
This chapter covers the principle, working, and operations of different types of chromatographic techniques like paper, thin layer, column...
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Urine and serum metabolomic analysis of endometrial cancer diagnosis and classification based on ultra-performance liquid chromatography mass spectrometry
ObjectiveThis study aimed to reveal the urinary and serum metabolic pattern of endometrial cancer (EC) and establish diagnostic models to identify EC...
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Isolation of Circulating Extracellular Vesicles by High-Performance Size-Exclusion Chromatography
Circulating extracellular vesicles (EVs) are gaining increased attention as carriers of proteins, nucleic acids, and lipids. Blood contains EVs from...