Abstract
Circulating extracellular vesicles (EVs) are gaining increased attention as carriers of proteins, nucleic acids, and lipids. Blood contains EVs from different cell sources that constitute an attractive target for biomarker studies. However, there is no consensus on the best approach to isolate EVs from blood. Non-EV proteins and lipoproteins in plasma/serum tend to contaminate isolated EVs and confound functional experiments. Here we describe a single-step, high-performance size-exclusion chromatography procedure for separation of EVs from most lipoproteins and non-EV proteins, and compare it to ultracentrifugation, still the most commonly used method for EV isolation.
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Acknowledgments
M. M. is a British Heart Foundation (BHF) Chair Holder (CH/16/3/32406) with BHF programme grant support (RG/16/14/32397). This work was supported by ERA-CVD Transnational Grant “MacroERA: Noncoding RNAs in cardiac macrophages and their role in heart failure,” and the Leducq Foundation (18CVD02).
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Takov, K., Teng, IJ., Mayr, M. (2022). Isolation of Circulating Extracellular Vesicles by High-Performance Size-Exclusion Chromatography. In: Federico, M., Ridolfi, B. (eds) Extracellular Vesicles in Diagnosis and Therapy. Methods in Molecular Biology, vol 2504. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2341-1_3
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DOI: https://doi.org/10.1007/978-1-0716-2341-1_3
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