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Enzyme Kinetics
In this chapter, you will learn the fundamental concepts of chemical and enzyme catalysis as well as kinetics such as the lock and key and the...
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Introduction of a Sodium-Binding Motif into Subunits a and c of Bacillus sp. PS3 Proton F-ATPase Does Not Result in Sodium Specificity of the Enzyme
AbstractIn bacteria, F-type ATPase (F-ATPase) plays a key role in bioenergetics and couples ATP synthesis/hydrolysis with the transport of ions (H + ...
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Map** the determinants of catalysis and substrate specificity of the antibiotic resistance enzyme CTX-M β-lactamase
CTX-M β-lactamases are prevalent antibiotic resistance enzymes and are notable for their ability to rapidly hydrolyze the extended-spectrum...
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Enzyme Purification
The growing importance of enzymes for the food, biotech, pharmaceutical, and cosmetics industries, among others, has increased the need for efficient...
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Enzyme Structure and Function
This chapter provides an overview on the general architecture of enzymes, describes protein structure and the types of enzyme cofactors, and...
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Oligomeric Structure of Yeast and Other Invertases Governs Specificity
Invertases, or β-fructofuranosidases, are metabolic enzymes widely distributed among plants and microorganisms that hydrolyze sucrose and release...
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Introduction to Enzyme Technology
Enzyme technology is one of the most important areas of modern biotechnology and involves numerous disciplines of natural sciences. It is a highly...
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Glycogen debranching pathway deduced from substrate specificity of glycogen debranching enzyme
Glycogen debranching enzyme (GDE) is bifunctional in that it exhibits both 4-α-glucanotransferase and amylo-α-1,6-glucosidase activity at two...
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Moonlighting enzymes: when cellular context defines specificity
It is not often realized that the absolute protein specificity is an exception rather than a rule. Two major kinds of protein multi-specificities are...
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Mycorrhizal specificity differences in epiphytic habitat: three epiphytic orchids harbor distinct ecological and physiological specificity
Orchidaceae has diversified in tree canopies and accounts for 68% of vascular epiphytes. Differences in mycorrhizal communities among epiphytic...
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Enzyme Identification and Screening: Activity-Based Methods
Methods for the identification and characterization of “ideal” enzymes for industrial use in the field of biotechnology have developed rapidly in...
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SfuPKS2, a novel type III polyketide synthase from Sargassum fusiforme, shows high substrate specificity
A type III polyketide synthase (SfuPKS2) from the edible seaweed Sargassum fusiforme was cloned and biochemically characterized. SfuPKS2 can only...
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ArdA Protein Specificity against Type I Restriction–Modification Systems
AbstractThe ArdA DNA-mimic antirestriction proteins inhibit type I restriction−modification (RMI) systems by binding instead of DNA to RMI. The ArdA...
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A pH-dependent shift of redox cofactor specificity in a benzyl alcohol dehydrogenase of aromatoleum aromaticum EbN1
AbstractWe characterise a reversible bacterial zinc-containing benzyl alcohol dehydrogenase (BaDH) accepting either NAD + or NADP + as a redox...
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Selection of extended CRISPR RNAs with enhanced targeting and specificity
As CRISPR effectors like Cas9 increasingly enter clinical trials for therapeutic gene editing, a future for personalized medicine will require...
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CasKAS: direct profiling of genome-wide dCas9 and Cas9 specificity using ssDNA map**
Detecting and mitigating off-target activity is critical to the practical application of CRISPR-mediated genome and epigenome editing. While numerous...
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In Silico Engineering of Enzyme Access Tunnels
Enzyme engineering is a tailoring process that allows the modification of naturally occurring enzymes to provide them with improved catalytic...
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Enzyme immobilization on covalent organic framework supports
Enzymes are natural catalysts with high catalytic activity, substrate specificity and selectivity. Their widespread utilization in industrial...
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Flexible active-site loops fine-tune substrate specificity of hyperthermophilic metallo-oxidases
Hyperthermophilic (‘superheat-loving’) archaea found in high-temperature environments such as Pyrobaculum aerophilum contain multicopper oxidases...
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Enzyme Modeling: From the Sequence to the Substrate Complex
This chapter gives an overview on computational methods to study the relationship between sequence, structure, and function of proteins....