Search
Search Results
-
Protein–Protein Interactions: Yeast Two Hybrid
The yeast two-hybridYeast two-hybrid (Y2H) systemTwo-hybrid system is a powerful and commonly used genetic tool to investigate the interaction...
-
Yeast Two-Hybrid Technique to Identify Protein–Protein Interactions
Protein–protein interactions are specific and direct physical contact between two or more proteins, and the interaction involves hydrogen bonding,...
-
Next-Generation Yeast Two-Hybrid Screening to Discover Protein–Protein Interactions
Yeast two-hybrid is a powerful approach to discover new protein–protein interactions. Traditional methods involve screening a target protein against...
-
Bioinformatic Analysis of Yeast Two-Hybrid Next-Generation Interaction Screen Data
Yeast two-hybrid next-generation interaction screening (Y2H-NGIS) uses the output of next-generation sequencing to mine for novel protein–protein...
-
Membrane and Nuclear Yeast Two-Hybrid Systems
The yeast two-hybrid (Y2H) system is an effective means of detecting protein–protein interactions through the activation of reporter gene expression....
-
High-Throughput Protein–Protein Interactions Screening Using Pool-Based Liquid Yeast Two-Hybrid Pipeline
Because of its adaptability to high-throughput approaches and a low operating cost, the yeast two-hybrid (Y2H) assay remains the most widely used one...
-
Detection of Protein–Protein Interactions Utilizing the Split-Ubiquitin Membrane-Based Yeast Two-Hybrid System
Identifying the interactors of a protein is a key step in understanding its possible cellular function(s). Among the various methods that can be used...
-
Protein–Protein Interaction: Bacterial Two Hybrid
The bacterial two-hybridBacterial two-hybrid (BACTH, for “Bacterial Adenylate Cyclase-based Two-Hybrid”) system is a simple and fast genetic approach...
-
Lager brewing yeast
Lager brewing yeast is a group of closely related strains of Saccharomyces pastorianus/S. carlsbergensis used for lager beer production all over the...
-
Dynamic Enrichment for Evaluation of Protein Networks (DEEPN): A High Throughput Yeast Two-Hybrid (Y2H) Protocol to Evaluate Networks
Proteins are the building blocks of life, and a vast array of cellular processes is handled by protein–protein interactions (PPIs). The protein...
-
Protein–Protein Interactions: Oxidative Bacterial Two Hybrid
Protein–protein interaction studies are essential to understand how proteins organize themselves into interaction networks and thus influence...
-
Identification of PDZ Interactions by Yeast Two-Hybrid Technique
The yeast two-hybrid technique is a powerful method to detect direct protein–protein interactions. Due to its accessibility, speed, and versatility,...
-
Screening of ABA Transporters by a Yeast Two-Hybrid System-Based Screening Using the Receptor Complex as a Sensor
Abscisic acid (ABA) is a plant hormone that is involved in many physiological events and is present in most plant tissues. How ABA moves within...
-
Detecting Blue Light-Dependent Protein–Protein Interactions by LexA-Based Yeast Two-Hybrid Assay
The LexA-based yeast two-hybrid system is one of the most powerful techniques used to detect blue light-dependent protein–protein interactions. In...
-
Protein–Protein Interactions: Bimolecular Fluorescence Complementation and Cytology Two Hybrid
Identifying protein–protein interactions between machine components of bacterial secretion systems and their cognate substrates is central to...
-
Recent co-evolution of two pandemic plant diseases in a multi-hybrid swarm
Most plant pathogens exhibit host specificity but when former barriers to infection break down, new diseases can rapidly emerge. For a number of...
-
A LexA-based yeast two-hybrid system for studying light-switchable interactions of phytochromes with their interacting partners
Phytochromes are a family of photoreceptors in plants that perceive the red (R) and far-red (FR) components of their light environment. Phytochromes...
-
Heterologous expression of Sesuvium portulacastrum SOS-related genes confer salt tolerance in yeast
Growth and development of plants are greatly affected by salinity. The salt overly sensitive (SOS) pathway plays a vital role in plants responding to...
-
From beer to breadboards: yeast as a force for biological innovation
The history of yeast Saccharomyces cerevisiae , aka brewer’s or baker’s yeast, is intertwined with our own. Initially domesticated 8,000 years ago to...
-
Screening of binding proteins that interact with two components of the HOG MAPK pathway by the yeast two-hybrid method in Colletotrichum siamense
In phytopathogenic fungi, the Hog1 MAPK pathway plays roles in osmoregulation, oxidation responses, fungicide sensitivity, among others. However, how...
