Search
Search Results
-
Complementation Assays for Co-chaperone Function
The development of mutant microorganisms lacking J domain proteins (JDPs; formerly called Hsp40s) has enabled the development of complementation...
-
Yeast-based system for in vivo evaluation of alleles of the anthocyanin production pathway
Plants produce anthocyanins to incite the pollination and seed dispersion performed by pigment-attracted animals. These natural blue-to-red-coloured...
-
Protein–Protein Interactions: Bimolecular Fluorescence Complementation and Cytology Two Hybrid
Identifying protein–protein interactions between machine components of bacterial secretion systems and their cognate substrates is central to...
-
Split complementation of base editors to minimize off-target edits
Base editors (BEs) empower the efficient installation of beneficial or corrective point mutations in crop and human genomes. However, conventional...
-
-
Non-Mitochondrial Aconitase-2 Mediates the Transcription of Nuclear-Encoded Electron Transport Chain Genes in Fission Yeast
Aconitase-2 (Aco2) is present in the mitochondria, cytosol, and nucleus of fission yeast. To explore its function beyond the well-known role in the...
-
From beer to breadboards: yeast as a force for biological innovation
The history of yeast Saccharomyces cerevisiae , aka brewer’s or baker’s yeast, is intertwined with our own. Initially domesticated 8,000 years ago to...
-
12 Baker’s Yeast: a rising foundation for eukaryotic sphingolipid-mediated cell signaling
Saccharomyces cerevisiae has been an invaluable tool for the dissection of sphingolipid metabolic pathways and cloning of enzymes involved in...
-
Current and novel approaches in yeast cell death research
The study of cell death mechanisms in fungi, particularly yeasts, has gained substantial interest in recent decades driven by the potential for...
-
Yeast Two-Hybrid Technique to Identify Protein–Protein Interactions
Protein–protein interactions are specific and direct physical contact between two or more proteins, and the interaction involves hydrogen bonding,...
-
6 Sterol biochemistry and regulation in the yeast Saccharomyces cerevisiae
Saccharomyces cerevisiae has been a major model system for the study of sterol biosynthesis and function. All of the genes encoding the enzymes...
-
The serine-arginine (SR) protein UmRrm75 from Ustilago maydis is a functional ortholog of yeast ScHrb1
The Basidiomycete fungus Ustilago maydis is a biotrophic pathogen of maize. The U. maydis UmRrm75 gene encodes an RNA-binding protein (RBP). In a...
-
Genetic analysis of DNA-damage tolerance pathways in Arabidopsis
Key messageGenetic analysis revealed a two-branch DNA-damage tolerance mechanism in Arabidopsis , namely translesion DNA synthesis and error-free...
-
Sequence, structural and functional conservation among the human and fission yeast ELL and EAF transcription elongation factors
BackgroundTranscription elongation is a dynamic and tightly regulated step of gene expression in eukaryotic cells. Eleven nineteen Lysine rich...
-
Increasing the number of ribosomal uL6 mRNA copies accelerates aging of the budding yeast
BackgroundAging is a biological process from which there is no escape. Diverse factors contribute to aging, most notably cell energy metabolism....
-
Ino2, activator of yeast phospholipid biosynthetic genes, interacts with basal transcription factors TFIIA and Bdf1
Binding of general transcription factors TFIID and TFIIA to basal promoters is rate-limiting for transcriptional initiation of eukaryotic...
-
Bioinformatic Analysis of Yeast Two-Hybrid Next-Generation Interaction Screen Data
Yeast two-hybrid next-generation interaction screening (Y2H-NGIS) uses the output of next-generation sequencing to mine for novel protein–protein...
-
Dynamic Enrichment for Evaluation of Protein Networks (DEEPN): A High Throughput Yeast Two-Hybrid (Y2H) Protocol to Evaluate Networks
Proteins are the building blocks of life, and a vast array of cellular processes is handled by protein–protein interactions (PPIs). The protein...
-
Bimolecular Fluorescence Complementation: Quantitative Analysis of In Cell Interaction of Nuclear Transporter Importin α with Cargo Proteins
Bimolecular fluorescence complementation utilizes the ability of two complementary nonfluorescent fragments to reconstitute and emit fluorescence...
-
Genetic Transformation of Cryptococcus Species with Agrobacterium Transfer DNA
Transformation of foreign DNA into Cryptococcus species is a powerful tool for exploring gene functions in these human pathogens. Agrobacterium...