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Mechanism of millisecond Lys48-linked poly-ubiquitin chain formation by cullin-RING ligases
E3 ubiquitin ligases, in collaboration with E2 ubiquitin-conjugating enzymes, modify proteins with poly-ubiquitin chains. Cullin-RING ligase (CRL)...
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Breaking the K48-chain: linking ubiquitin beyond protein degradation
The discovery of ubiquitin conjugation to lysines and the role of K48-linked polyubiquitin in targeting substrates for proteasomal degradation was...
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Topology of Ubiquitin Chains in the Chromatosomal Environment of the E3 Ubiquitin Ligase RNF168
AbstractGenome stability is critical for normal functioning of cells, it depends on accuracy of DNA replication, chromosome segregation, and DNA...
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Current methodologies in protein ubiquitination characterization: from ubiquitinated protein to ubiquitin chain architecture
Ubiquitination is a versatile post-translational modification (PTM), which regulates diverse fundamental features of protein substrates, including...
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The ubiquitin-proteasome pathway in cell cycle control
Ubiquitin-mediated proteolysis is one of the key mechanisms underlying cell cycle control. The removal of barriers posed by accumulation of...
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Analysis of Proteasome-Associated Ubiquitin Ligase Activity
The ubiquitin-proteasome system (UPS) is the predominant protein degradation machinery in eukaryotic cells. It is highly conserved among eukaryotes...
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Complex Topology of Ubiquitin Chains Mediates Lysosomal Degradation of MrgC Proteins
BackgroundActivation of Mas-related G protein-coupled receptor C (MrgC) receptors relieves pain, but also leads to ubiquitination of MrgC receptors....
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Broad-spectrum ubiquitin/ubiquitin-like deconjugation activity of the rhizobial effector NopD from Bradyrhizobium (sp. XS1150)
The post-translational modification of proteins by ubiquitin-like modifiers (UbLs), such as SUMO, ubiquitin, and Nedd8, regulates a vast array of...
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Identification and Characterization of Physiological Pairing of E2 Ubiquitin-Conjugating Enzymes and E3 Ubiquitin Ligases
The posttranslational attachment of the small protein modifier ubiquitin (Ub) is best known for its function in targeting proteins for degradation by...
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An expanded lexicon for the ubiquitin code
Our understanding of the ubiquitin code has greatly evolved from conventional E1, E2 and E3 enzymes that modify Lys residues on specific substrates...
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Arabidopsis thaliana ubiquitin-associated protein 2 (AtUAP2) functions as an E4 ubiquitin factor and negatively modulates dehydration stress response
E4, a ubiquitin (Ub) chain assembly factor and post-translational modification protein, plays a key role in the regulation of multiple cellular...
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Mitigation of the Deleterious Effect of Heavy Metals on the Conformational Stability of Ubiquitin through Osmoprotectants
The Ubiquitin-Proteasome System (UPS) is important in protein homeostasis and is involved in many cell processes. UPS’s wide range of regulatory...
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Analyzing Protein–Protein Interactions Using the Split-Ubiquitin System
The split-ubiquitin technology was developed over 20 years ago as an alternative to Gal4-based, yeast-two-hybrid methods to identify interacting...
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A context-dependent and disordered ubiquitin-binding motif
Ubiquitin is a small, globular protein that is conjugated to other proteins as a posttranslational event. A palette of small, folded domains...
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N4BP1 functions as a dimerization-dependent linear ubiquitin reader which regulates TNF signalling
Signalling through TNFR1 modulates proinflammatory gene transcription and programmed cell death, and its impairment causes autoimmune diseases and...
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The Role of Ubiquitin–Proteasome System in the Biology of Stem Cells
AbstractSelective degradation of cellular proteins by the ubiquitin–proteasome system (UPS) is one of the key regulatory mechanisms in eukaryotic...
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Chlorogenic acid modulates the ubiquitin–proteasome system in stroke animal model
BackgroundChlorogenic acid, a phenolic compound, has potent antioxidant and neuroprotective properties. The ubiquitin–proteasome system is an...
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Purification and Detection of Ubiquitinated Plant Proteins Using Tandem Ubiquitin Binding Entities
The timing and amplitude of plant signaling are frequently regulated through posttranslational modification of key signaling sectors, which...
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LIMA1 links the E3 ubiquitin ligase RNF40 to lipid metabolism
LIMA1 is a LIM domain and Actin binding 1 protein that acts as a skeleton protein to promote cholesterol absorption, which makes it an ideal target...
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Concerted SUMO-targeted ubiquitin ligase activities of TOPORS and RNF4 are essential for stress management and cell proliferation
Protein SUMOylation provides a principal driving force for cellular stress responses, including DNA–protein crosslink (DPC) repair and...
