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Constructed Rice Tracers Identify the Major Virulent Transcription Activator-Like Effectors of the Bacterial Leaf Blight Pathogen
Xanthomonas oryzae pv. oryzae ( Xoo ) injects major transcription activator-like effectors (TALEs) into plant cells to activate susceptibility ( S )...
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TALE-based organellar genome editing and gene expression in plants
Key messageTALE-based editors provide an alternative way to engineer the organellar genomes in plants. We update and discuss the most recent...
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Diversity of transcription activator-like effectors and pathogenicity in strains of Xanthomonas oryzae pv. oryzicola from Yunnan
The present study aimed to evaluate transcriptional activator-like effector ( TALE ) genes in 86 Xanthomonas oryzae pv. oryzicola strains collected...
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Targeted C•G-to-T•A base editing with TALE-cytosine deaminases in plants
BackgroundTALE-derived DddA-based cytosine base editors (TALE-DdCBEs) can perform efficient base editing of mitochondria and chloroplast genomes....
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TALE-induced immunity against the bacterial blight pathogen Xanthomonas oryzae pv. oryzae in rice
Transcription activator-like effectors (TALEs) are proteins produced by plant pathogenic Xanthomonas spp. TALEs exhibit a conserved structure and...
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Design and Application of DNA Modification-Specific Transcription-Activator-Like Effectors
Transcription-activator like effectors (TALEs) are DNA-binding proteins used for genome targeting. TALEs contain a central domain of concatenated... -
Epigenetic Manipulation of Transposable and Repetitive Elements
This protocol describes methods to design, assemble, and validate tools for targeted activation or repression of single-copy and multi-copy genes,... -
Updated Overview of TALEN Construction Systems
Transcription activator-like effector (TALE) nuclease (TALEN) is the second-generation genome editing tool consisting of TALE protein containing... -
Epigenetic features improve TALE target prediction
BackgroundThe yield of many crop plants can be substantially reduced by plant-pathogenic Xanthomonas bacteria. The infection strategy of many Xanthomonas...
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Engineering the plastid and mitochondrial genomes of flowering plants
Engineering the plastid genome based on homologous recombination is well developed in a few model species. Homologous recombination is also the rule...
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Strand-selective base editing of human mitochondrial DNA using mitoBEs
A number of mitochondrial diseases in humans are caused by point mutations that could be corrected by base editors, but delivery of CRISPR guide RNAs...
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Assembling highly repetitive Xanthomonas TALomes using Oxford Nanopore sequencing
BackgroundMost plant-pathogenic Xanthomonas bacteria harbor transcription activator-like effector (TALE) genes, which function as transcriptional...
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Comparative transcriptomic profiling of the two-stage response of rice to Xanthomonas oryzae pv. oryzicola interaction with two different pathogenic strains
BackgroundTwo-tiered plant immune responses involve cross-talk among defense-responsive ( DR ) genes involved in pathogen-associated molecular pattern...
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Plant Genome Editing Technologies: An Updated Overview
A genome is a representation of all the genetic material that forms an organism and is stored in its deoxyribonucleic acid (DNA). Genetic... -
Genome-Editing Technologies in Crop Improvement
Global climate change, together with harmful biotic and abiotic factors, is limiting agricultural yields, rendering it challenging to meet the rising... -
Characterization of Mammalian Regulatory Complexes at Single-Locus Resolution Using TINC
In mammalian cells, multiprotein complexes form at specific genomic regulatory elements (REs) to control gene expression, which in turn is ultimately... -
Transgenic Strategies and Genome Editing for Nutritional Enrichment
The agriculture sector is facing severe challenges in producing high-quality food materials in significant quantities because of climate change and... -
Genetic Engineering and Gene Editing for Targeted Trait Modifications
In the twenty-first century, the scientific community faces the challenge of ensuring food security for an increasing population while tackling... -
TAL effectors and the predicted host targets of pomegranate bacterial blight pathogen Xanthomonas citri pv. punicae
The molecular mechanism of pomegranate susceptibility to bacterial blight, a serious threat to pomegranate production in India, is largely unknown....
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Application of CRISPR-Cas9 Technology in Fish
CRISPR/Cas9-based genome editing allows efficient and targeted enhancement of important aquaculture traits and will probably be the solution to the...