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Mammalian PIWI–piRNA–target complexes reveal features for broad and efficient target silencing
The PIWI-interacting RNA (piRNA) pathway is an adaptive defense system wherein piRNAs guide PIWI family Argonaute proteins to recognize and silence...
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Prediction of on-target and off-target activity of CRISPR–Cas13d guide RNAs using deep learning
Transcriptome engineering applications in living cells with RNA-targeting CRISPR effectors depend on accurate prediction of on-target activity and...
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MicroRNA Detection and Target Identification Methods and Protocols
This updated volume reflects new and evolved techniques to study detection, profiling, and manipulation of microRNAs (miRNAs) in plants and animals....
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Predator-avoidance behaviour of target and non-target temperate reef fishes is lower in areas protected from fishing
The effects of hunting on predator-avoidance behaviour are increasingly being recognised on land but have received less attention in marine systems....
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Target-Based Screening for Lead Discovery
As a result of the enormous advances in molecular biology, recombinant technology, and genomics over the past few decades, target-based drug...
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Split complementation of base editors to minimize off-target edits
Base editors (BEs) empower the efficient installation of beneficial or corrective point mutations in crop and human genomes. However, conventional...
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Comparative genome-wide analysis of circular RNAs in Brassica napus L.: target-site versus non-target-site resistance to herbicide stress
Circular RNAs (circRNAs), a class of non-coding RNA molecules, are recognized for their unique functions; however, their responses to herbicide...
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Undetectable off-target effects induced by FokI catalytic domain in mouse embryos
The FokI catalytic domain can be fused to various DNA binding architectures to improve the precision of genome editing tools. However, evaluation of...
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Complicated target recognition by archaeal box C/D guide RNAs
Box C/D RNAs guide the site-specific formation of 2′- O -methylated nucleotides (Nm) of RNAs in eukaryotes and archaea. Although C/D RNAs have been...
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Tracking-seq reveals the heterogeneity of off-target effects in CRISPR–Cas9-mediated genome editing
The continued development of novel genome editors calls for a universal method to analyze their off-target effects. Here we describe a versatile...
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Integration of landmark and saccade target signals in macaque frontal cortex visual responses
Visual landmarks influence spatial cognition and behavior, but their influence on visual codes for action is poorly understood. Here, we test...
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Target-based discovery of a broad-spectrum flukicide
Diseases caused by parasitic flatworms impart a considerable healthcare burden worldwide. Many of these diseases—for example, the parasitic blood...
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CHEK2 knockout is a therapeutic target for TP53-mutated hepatocellular carcinoma
Currently, there is still a lack of novel and effective drug targets to improve the prognosis of hepatocellular carcinoma (HCC). Additionally, the...
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Improvement of Crops Using the CRISPR/Cas System: New Target Genes
AbstractThe success of genome editing of crops using the CRISPR/Cas system largely depends on the correct choice of target genes, for which directed...
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MicroRNA–Target Identification: A Combinatorial In Silico Approach
Contemporary computational target prediction tools with their distinctive properties and stringency have been playing a vital role in pursuing...
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TEC-miTarget: enhancing microRNA target prediction based on deep learning of ribonucleic acid sequences
BackgroundMicroRNAs play a critical role in regulating gene expression by binding to specific target sites within gene transcripts, making the...
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Single-base tiled screen unveils design principles of PspCas13b for potent and off-target-free RNA silencing
The development of precise RNA-editing tools is essential for the advancement of RNA therapeutics. CRISPR (clustered regularly interspaced short...
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PKMYT1: A Potential Target for CCNE1 Amplificated Colorectal Tumors
Colorectal cancer is a malignant tumor with higher morbidity and mortality. The purpose of this study is to investigate whether inhibition of Protein...
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MOKPE: drug–target interaction prediction via manifold optimization based kernel preserving embedding
BackgroundIn many applications of bioinformatics, data stem from distinct heterogeneous sources. One of the well-known examples is the identification...
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ALK-positive lung cancer: a moving target
Anaplastic lymphoma kinase (ALK) is a potent oncogenic driver in lung cancer. ALK tyrosine kinase inhibitors yield significant benefit in patients...
