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Cationic lipid nanoparticle-mediated delivery of a Cas9/crRNA ribonucleoprotein complex for transgene-free editing of the citrus plant genome
Key messageA lipofectamine-mediated transfection protocol for DNA-free genome editing of citrus protoplast cells using a Cas9/gRNA ribonucleoprotein...
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Ribonucleoprotein (RNP)-Mediated Targeted Mutagenesis in Barley (Hordeum vulgare L.)
The crop species barley is a genetic model for the small grain temperate cereals. Thanks to the availability of whole genome sequence and the...
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Direct observation of translational activation by a ribonucleoprotein granule
Biomolecular condensates organize biochemical processes at the subcellular level and can provide spatiotemporal regulation within a cell. Among...
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Immunoprecipitation Methods to Isolate Messenger Ribonucleoprotein Complexes (mRNP)
Throughout their life cycle, messenger RNAs (mRNAs) associate with proteins to form ribonucleoproteins (mRNPs). Each mRNA is part of multiple...
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Ribonucleoprotein (RNP)-Mediated Allele Replacement in Barley (Hordeum vulgare L.) Leaves
Varietal differences within a species with agronomic importance are often based on minor changes in the genomic sequence. For example,...
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Efficient Editing of the CXCR4 Locus Using Cas9 Ribonucleoprotein Complexes Stabilized with Polyglutamic Acid
AbstractGene editing using the CRISPR/Cas9 system provides new opportunities to treat human diseases. Approaches aimed at increasing the efficiency...
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Ribonucleoprotein Complex Factor Ybx1 Stabilizes the Maternal mRNA of the ssx2ip Gene Encoding the Centrosome Maturation Protein in Xenopus laevis Embryogenesis
AbstractObjective: This study continues our investigation into the mechanisms that control early developmental gene expression in Xenopus laevis ...
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Antiviral Activity of CRISPR/Cas9 Ribonucleoprotein Complexes on a Hepatitis B Virus Model In Vivo
AbstractChronic hepatitis B (CHB) is caused by hepatitis B virus (HBV) infection. This disease is a key issue for global health. Modern methods of...
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Membrane surfaces regulate assembly of ribonucleoprotein condensates
Biomolecular condensates organize biochemistry, yet little is known about how cells control the position and scale of these structures. In cells,...
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Genome Editing in Chlamydomonas reinhardtii Using Cas9-gRNA Ribonucleoprotein Complex: A Step-by-Step Guide
Genome editing technologies have provided opportunities to manipulate literally any genomic location, opening new avenues for reverse genetics-based...
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Structural insights into ribonucleoprotein dissociation by nucleocapsid protein interacting with non-structural protein 3 in SARS-CoV-2
The coronavirus nucleocapsid (N) protein interacts with non-structural protein 3 (Nsp3) to facilitate viral RNA synthesis and stabilization. However,...
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Genome Editing by Ribonucleoprotein Based Delivery of the Cas9 System in Plants
Clustered regularly interspaced palindromic repeat (CRISPR)-based gene editing technology has opened the doors for targeted mutagenesis in a variety...
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Engineered virus-like particles for transient delivery of prime editor ribonucleoprotein complexes in vivo
Prime editing enables precise installation of genomic substitutions, insertions and deletions in living systems. Efficient in vitro and in vivo...
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Lipofection-Based Delivery of CRISPR/Cas9 Ribonucleoprotein for Gene Editing in Male Germline Stem Cells
Gene editing in the murine germline is a valuable approach to investigate germ cell maturation and generate mouse models. Several studies...
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Generation of double knockout cattle via CRISPR-Cas9 ribonucleoprotein (RNP) electroporation
BackgroundGenome editing has been considered as powerful tool in agricultural fields. However, genome editing progress in cattle has not been fast as...
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Editing of the ethylene biosynthesis gene in carnation using CRISPR-Cas9 ribonucleoprotein complex
The study aimed to edit ethylene (ET) biosynthesis genes [ 1-aminocyclopropane-1-carboxylic acid (ACC) synthetase 1 ( ACS1 ) and ACC oxidase 1 ( ACO1 )]...
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Using Ribonucleoprotein-based CRISPR/Cas9 to Edit Single Nucleotide on Human Induced Pluripotent Stem Cells to Model Type 3 Long QT Syndrome (SCN5A±)
Human induced pluripotent stem cells (hiPSCs) have been widely used in cardiac disease modelling, drug discovery, and regenerative medicine as they...
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Targeted Gene Disruption Via CRISPR/Cas9 Ribonucleoprotein Complexes in Fusarium oxysporum
Characterization of a gene of interest frequently relies on generation of a mutant as a critical component. Transformation to disrupt a gene has been...
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Optimization of CRISPR/Cas9 ribonucleoprotein delivery into cabbage protoplasts for efficient DNA-free gene editing
The CRISPR/Cas9-based gene editing system for the direct delivery of pre-assembled Cas9 ribonucleoproteins (RNPs), consisting of a Cas9 nuclease and...
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Establishment of a PEG-Assisted Protoplast Transfection System in Musa acuminata cv. Berangan (AAA) Using a CRISPR/Cas9 Ribonucleoprotein Complex
AbstractBanana is an important food crop worldwide. However, as banana production is largely affected by pests, diseases, and environmental stresses,...
