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Laser Sources for Traditional and Spectral Flow Cytometry
Lasers for light scattering measurement and fluorescence excitation are essential components of all flow cytometers. Flow cytometers now typically...
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Rapid ex vivo molecular fingerprinting of biofluids using laser-assisted rapid evaporative ionization mass spectrometry
Of the many metabolites involved in any clinical condition, only a narrow range of biomarkers is currently being used in the clinical setting. A key...
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General Considerations for Acquiring a Three-Color Image by Laser Scanning Confocal Microscopy
Laser scanning confocal microscopy is the workhorse epifluorescence imaging technique used in laboratories worldwide to acquire three-dimensional...
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Pulsed stimulated Brillouin microscopy enables high-sensitivity mechanical imaging of live and fragile biological specimens
Brillouin microscopy is an emerging optical elastography technique capable of assessing mechanical properties of biological samples in a...
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High-resolution line-scan Brillouin microscopy for live imaging of mechanical properties during embryo development
Brillouin microscopy can assess mechanical properties of biological samples in a three-dimensional (3D), all-optical and hence non-contact fashion,...
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Using Gas Exchange to Study CO2 Release During Photosynthesis with Steady- and Nonsteady-State Approaches
Measures of respiration in the light and Ci* are crucial to the modeling of photorespiration and photosynthesis. This chapter provides background on...
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More than double the fun with two-photon excitation microscopy
For generations researchers have been observing the dynamic processes of life through the lens of a microscope. This has offered tremendous insights...
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Build and operation of a custom 3D, multicolor, single-molecule localization microscope
Single-molecule localization microscopy (SMLM) enables imaging scientists to visualize biological structures with unprecedented resolution....
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Single-Cell Resolution Optogenetics Via Expression of Soma-Targeted Rhodopsins
Optogenetics allows control of neural activity in genetically targeted neuron populations by light. Optogenetic control of individual neurons in...
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Super-Resolution Imaging of the Actin Cytoskeleton in Living Cells Using TIRF-SIM
Super-resolution (SR) imaging techniques have advanced rapidly in recent years, but only a subset of these techniques is gentle enough to be used by...
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Event-triggered STED imaging
Monitoring the proteins and lipids that mediate all cellular processes requires imaging methods with increased spatial and temporal resolution. STED...
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Rapid biomechanical imaging at low irradiation level via dual line-scanning Brillouin microscopy
Brillouin microscopy is a technique for mechanical characterization of biological material without contact at high three-dimensional resolution....
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Bioconjugates of photon-upconversion nanoparticles for cancer biomarker detection and imaging
The detection of cancer biomarkers in histological samples and blood is of paramount importance for clinical diagnosis. Current methods are limited...
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Optogenetic Control of Human Stem Cell-Derived Neurons
Spontaneous and optogenetically evoked activities of human induced pluripotent stem cell (hiPSC)-derived neurons can be assessed by patch clamp and...
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Optimal precision and accuracy in 4Pi-STORM using dynamic spline PSF models
Coherent fluorescence imaging with two objective lenses (4Pi detection) enables single-molecule localization microscopy with sub-10 nm spatial...
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Graphene- and metal-induced energy transfer for single-molecule imaging and live-cell nanoscopy with (sub)-nanometer axial resolution
Super-resolution fluorescence imaging that surpasses the classical optical resolution limit is widely utilized for resolving the spatial organization...
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Quantitative assessment of near-infrared fluorescent proteins
Recent progress in fluorescent protein development has generated a large diversity of near-infrared fluorescent proteins (NIR FPs), which are rapidly...
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Rapid and Non-Invasive Bio-Photonic Techniques for Morphological and Functional State Study of Lycopene in Tomato and Tomato Products
AbstractThe natural red pigment compound of tomato is lycopene which is responsible for red pigment in some fruits and vegetables. We have determined...
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Genetically encoded photo-switchable molecular sensors for optoacoustic and super-resolution imaging
Reversibly photo-switchable proteins are essential for many super-resolution fluorescence microscopic and optoacoustic imaging methods. However, they...
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The Role of Plant Doctors in Food Security, Safety, and Plant Health Regulation
The Anthropocene poses numerous challenges at the environmental-agricultural-social interface. The confluence of recent events has provided a case...