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Laser Sources for Traditional and Spectral Flow Cytometry

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Flow Cytometry Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2779))

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Abstract

Lasers for light scattering measurement and fluorescence excitation are essential components of all flow cytometers. Flow cytometers now typically rely on multiple laser wavelengths allowing excitation of a constantly increasing variety of fluorescent probes. The expanding use of spectral flow cytometry to increase the magnitude of multiparametric analysis is also changing the significance of laser choice in cytometry. In this chapter, we review the lasers available for flow cytometry and provide guidance in choosing laser wavelengths and characteristics to best match the needs of modern cell analysis by both conventional and spectral cytometry. We also discuss the recent advances in laser technology as the push to expand the palette of laser wavelength for cytometry continues.

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Author information

Authors and Affiliations

  1. Laboratory of Pathology, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA

    William G. Telford

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  1. William G. Telford
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Correspondence to William G. Telford .

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Editors and Affiliations

  1. Bethesda, MD, USA

    Teresa S. Hawley

  2. Washington, DC, USA

    Robert G. Hawley

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Telford, W.G. (2024). Laser Sources for Traditional and Spectral Flow Cytometry. In: Hawley, T.S., Hawley, R.G. (eds) Flow Cytometry Protocols. Methods in Molecular Biology, vol 2779. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3738-8_3

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  • DOI: https://doi.org/10.1007/978-1-0716-3738-8_3

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3737-1

  • Online ISBN: 978-1-0716-3738-8

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