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Improving the sensitivity of in vivo CRISPR off-target detection with DISCOVER-Seq+
Discovery of off-target CRISPR–Cas activity in patient-derived cells and animal models is crucial for genome editing applications, but currently...
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The importance of murine phospho-MLKL-S345 in situ detection for necroptosis assessment in vivo
Necroptosis is a caspase-independent modality of cell death implicated in many inflammatory pathologies. The execution of this pathway requires the...
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In Vivo Imaging of Bone Marrow Long-Chain Fatty Acid Uptake
In vivo imaging enables the detection and visualization of many different processes occurring within the body. Fatty acid uptake is a fundamental...
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Investigation of sRNA-mRNA Interactions in Bacillus subtilis In Vivo
In this chapter, we describe in vivo methods for the analysis of interactions between an sRNA and its target mRNA in B. subtilis. All these methods...
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Measurement of Phytochrome B Thermal Reversion Rates In Vivo
Thermal reversion of phytochromes is the light-independent but strongly temperature-dependent relaxation of the light-activated Pfr form of...
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Post-click labeling enables highly accurate single cell analyses of glucose uptake ex vivo and in vivo
Cellular glucose uptake is a key feature reflecting metabolic demand of cells in physiopathological conditions. Fluorophore-conjugated sugar...
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In Vivo Tracking of Dendritic Cell Migration
Dendritic cells (DCs) in peripheral tissue serve as a sentinel to invasion and maintain tolerance. They ingest and carry antigens to the draining...
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Evaluation of Connexin Hemichannel Activity In Vivo
Connexin hemichannels (Cx HCs) are hexameric structures at the cell plasma membrane, whose function as membrane transport proteins allows for the...
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UFObow: A single-wavelength excitable Brainbow for simultaneous multicolor ex-vivo and in-vivo imaging of mammalian cells
Brainbow is a genetic cell-labeling technique that allows random colorization of multiple cells and real-time visualization of cell fate within a...
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Genetically encoded phosphatidylserine biosensor for in vitro, ex vivo and in vivo labelling
BackgroundThe dynamics of phosphatidylserine in the plasma membrane is a tightly regulated feature of eukaryotic cells. Phosphatidylserine (PS) is...
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In-vivo Cross-linking of Biotinylated Peptide Ligands to Cell Surface Receptors
In-vivo cross-linking of biotinylated peptides is a technique to analyze the interaction of small proteins or peptide ligands with their...
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Molecular Imaging for In Vivo Tracking and Detection of Galectin Binding Partners
Molecular imaging (MI) is a non-invasive growing technology that allows the investigation of cellular and molecular processes in basic and clinical...
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Evaluation of the In Vitro and In Vivo Effects of Biglycan in Innate Immunity
Biglycan, a small leucine-rich proteoglycan (SLRP), is a crucial component of the extracellular matrix (ECM) associated with the maintenance of...
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Current capabilities and future perspectives of FCS: super-resolution microscopy, machine learning, and in vivo applications
Fluorescence correlation spectroscopy (FCS) is a single molecule sensitive tool for the quantitative measurement of biomolecular dynamics and...
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In Vivo Detection of Double-Stranded RNA by dRBFC Assay
Double-stranded RNA (dsRNA) is the genomic material or replicate intermediate of RNA viruses, and is also a crucial signal molecule to trigger innate...
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Sensitive multicolor indicators for monitoring norepinephrine in vivo
Genetically encoded indicators engineered from G-protein-coupled receptors are important tools that enable high-resolution in vivo neuromodulator...
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In Vivo Analysis of ER-Associated Protein Degradation and Ubiquitination in Arabidopsis thaliana
The endoplasmic reticulum (ER) is the cellular site for the biosynthesis of proteins and lipids. The ER is highly dynamic, whose homeostasis is...
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Site-Directed Cross-Linking Between Bacterial Flagellar Motor Proteins In Vivo
The bacterial flagellum employs a rotary motor embedded on the cell surface. The motor consists of the stator and rotor elements and is driven by ion...
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Metabolism-driven in vitro/in vivo disconnect of an oral ERɑ VHL-PROTAC
Targeting the estrogen receptor alpha (ERα) pathway is validated in the clinic as an effective means to treat ER+ breast cancers. Here we present the...
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Iron-Related Genes and Proteins in Mesenchymal Stem Cell Detection and Therapy
Mesenchymal stem cells (MSCs) are located in various tissues of the body. These cells exhibit regenerative and reparative properties, which makes...
