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High-yield synthesis of 2-O-α-d-glucosyl-d-glycerate by a bifunctional glycoside phosphorylase
AbstractOsmolytes are produced by various microorganisms as a defense mechanism to protect cells and macromolecules from damage caused by external...
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Structural elucidation a complex galactosyl and glucosyl-rich pectin from the pericarp of immature fruits of Juglans mandshurica Maxim.
A glucosyl-rich pectin, JMMP-3 ( M w , 2.572 × 10 4 g/mol, O -methyl % = 3.62%), was isolated and purified from the pericarp of the immature fruit of Juglan...
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Structure and sucrose binding mechanism of the plant SUC1 sucrose transporter
Sucrose import from photosynthetic tissues into the phloem is mediated by transporters from the low-affinity sucrose transporter family (SUC/SUT...
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Enhancement of debitterness, water-solubility, and neuroprotective effects of naringin by transglucosylation
AbstractNaringin found in citrus fruits is a flavanone glycoside with numerous biological activities. However, the bitterness, low water-solubility,...
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Chemical Synthesis of Gangliosides
Synthetic methodologies for gangliosides have evolved over the past three decades. The strategies for constructing ganglioside skeletons can... -
Virtual Screening Technique to Identify Inhibitors of Mycobacterium tuberculosis Rv3032 Protein Involved in MGLP Biosynthesis
AbstractObjective: Tuberculosis (TB) is a contagious disease caused by Mycobacterium tuberculosis , that poses a threat to the international...
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Nitrogen-Containing Compounds
Nitrogen-containing compounds include three main metabolic classes: alkaloids, cyanogenic glucosides and glucosinolates. Alkaloids show the most... -
A practical approach to producing isomaltomegalosaccharide using dextran dextrinase from Gluconobacter oxydans ATCC 11894
AbstractDextran dextrinase (DDase) catalyzes formation of the polysaccharide dextran from maltodextrin. During the synthesis of dextran, DDase also...
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Glycogen debranching pathway deduced from substrate specificity of glycogen debranching enzyme
Glycogen debranching enzyme (GDE) is bifunctional in that it exhibits both 4-α-glucanotransferase and amylo-α-1,6-glucosidase activity at two...
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Producing 2-O-α-D-glucopyranosyl-L-ascorbic acid by modified cyclodextrin glucosyltransferase and isoamylase
AbstractIn this study, site saturation mutagenesis was performed on the − 3 (R44, D86, S90, and D192) and − 6 subsite (Y163, G175, G176, and N189) of
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Regioselective O-acetylation of various glucosides catalyzed by Escherichia coli maltose O-acetyltransferase
AbstractEscherichia coli , a well-known prokaryotic organism, has been widely employed as a versatile host for heterologous overexpression of...
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Research progress and application of enzymatic synthesis of glycosyl compounds
AbstractGlucoside compounds are widely found in nature and have garnered significant attention in the medical, cosmetics, and food industries due to...
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Mechanistic insights into granule-bound starch synthase I (GBSSI.L539P) allele in high amylose starch biosynthesis in wheat (Triticum aestivum L.)
Amylose fraction of grain starch is correlated with a type of resistant starch with better nutritional quality. Granule-bound starch synthase I...
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New seco-anthraquinone glucoside from the roots of Rumex crispus
A new seco-anthraquinone, crispuside A ( 1 ), and three new 3,4-dihydronaphthalen-1(2 H )-ones, napthalenones A-C ( 2 – 4 ), were isolated from the roots of Ru...
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Enhancing thermostability and activity of sucrose phosphorylase for high-level production of 2-O-α-d-glucosylglycerol
Sucrose phosphorylase (SPase) can transfer the glucosyl group of sucrose to different compounds and has been widely used in industry. To overcome the...
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Metabolism of Storage Carbohydrates
Besides playing other roles, carbohydrates are also the major source of energy for all living beings. Almost 30% of the carbohydrates in plants are... -
Multifaceted roles of plant glycosyl hydrolases during pathogen infections: more to discover
Main conclusionCarbohydrates are hydrolyzed by a family of carbohydrate-active enzymes (CAZymes) called glycosidases or glycosyl hydrolases. Here, we...
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Glucansucrases Derived from Lactic Acid Bacteria to Synthesize Multitudinous α-Glucans
Lactic acid bacteria (LAB) are widely applied in the industrial production of fermented foods, such as pickles, yoghurt, and soy sauce, due to their... -
The Arabidopsis AtSWEET13 transporter discriminates sugars by selective facial and positional substrate recognition
Transporters are targeted by endogenous metabolites and exogenous molecules to reach cellular destinations, but it is generally not understood how...
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Profiling the cell walls of seagrasses from A (Amphibolis) to Z (Zostera)
BackgroundThe polyphyletic group of seagrasses shows an evolutionary history from early monocotyledonous land plants to the marine environment....