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The GUS Reporter System in Flower Development Studies
The β-glucuronidase (GUS) reporter gene system is an important technique with versatile uses in the study of flower development in a broad range of...
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Evaluation of two new promoters to express transgenes stably in lettuce (Lactuca sativa L.)
Several studies suggest that transgene expression in lettuce may be unstable, possibly as a result of epigenetic silencing of the cauliflower mosaic...
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Salicylic Acid and H2O2 Induce PPO Derived GUS Expression in Arabidopsis
AbstractPolyphenol oxidases (PPOs) are ubiquitous enzymes of plant defense which transform polyphenols into quinones that response to biotic and...
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Molecular Cloning and Functional Analysis of ScHAK10 Gene Promoter from Sugarcane (Saccharum officinarum L.)
Transcriptional regulation of the high-affinity K + ( HAK ) transporter gene is an important mechanism of plant response to low potassium stress....
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Identification of a sugarcane bacilliform virus promoter that is activated by drought stress in plants
Sugarcane ( Saccharum spp.) is an important sugar and biofuel crop in the world. It is frequently subjected to drought stress, thus causing...
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PSG076 Promoter Shows Late Pollen-Specific Activity in Wheat (Triticum aestivum L.)
Pollen, as the male gametophyte of plants, plays a critical role in plant sexual reproduction. Investigating pollen-specific promoters not only helps...
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Identification of a novel promoter region responsible for the embryo-specific expression of SERK1 in pineapple
Somatic embryogenesis (SE) is a key regeneration process in plant. AcSERK1 is a gene specifically expressed in the early stage of SE in pineapple ( Ana...
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Cloning and functional analysis of the promoter of a UDP-glycosyltransferase gene from Panax quinquefolium L.
Pq3-O-UGT2 is a key UDP-glycosyltransferase gene in the ginsenoside biosynthesis pathway of Panax quinquefolium L. Functional analysis of promoter...
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High-level expression of sucrose inducible sweet potato sporamin gene promoter: β-glucuronidase fusion gene in transgenic Nicotiana plumbaginifolia hairy roots
We developed transgenic Nicotiana plumbaginifolia hairy roots with sucrose-inducible minimal promoter (Spo min )-β-glucuronidase (GUS) gene–fused...
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A rapid and efficient Agrobacterium-mediated transient transformation system in grape berries
Transient transformation is extremely useful for rapid in vivo assessment of gene function, especially for fruit-related genes. Grape berry, while an...
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Functional characterization of GhNAC2 promoter conferring hormone- and stress-induced expression: a potential tool to improve growth and stress tolerance in cotton
The GhNAC2 transcription factor identified from G. herbaceum improves root growth and drought tolerance through transcriptional reprogramming of...
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Suppression of the dwarf phenotype of an Arabidopsis mutant defective in thermospermine biosynthesis by a synonymous codon change in the SAC51 uORF
Thermospermine plays a critical role in negatively regulating xylem development in angiosperms. A mutant of Arabidopsis thaliana that is defective in...
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An effective transient expression system for gene function identification in Lotus japonicus
Transient transformation is a notably efficient and expeditious method for obtaining foreign gene expression products, making it a prominent avenue...
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3,4-Dichlorophenylacetic acid acts as an auxin analog and induces beneficial effects in various crops
Auxins and their analogs are widely used to promote root growth, flower and fruit development, and yield in crops. The action characteristics and...
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GUS-reporter based analysis of the promoter activity of Gossypium hirsutum NAC transcription factor, GhNAC4 that is induced by phytohormones and environmental stresses
The expression analysis of GhNAC4 , a NAC ( NAM, ATAF1 - 2 , and CUC2 ) domain-containing transcription factor of the upland cotton, Gossypium hirsutum was...
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Unveiling the defensive role of Snakin-3, a member of the subfamily III of Snakin/GASA peptides in potatoes
Key messageThe first in-depth characterization of a subfamily III Snakin/GASA member was performed providing experimental evidence on promoter...
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Quantitative Estimation of Promoter Activity in Cannabis sativa Using Agroinfiltration-Based Transient Gene Expression
To properly assess promoter activity, which is critical for understanding biosynthetic pathways in different plant species, we use...
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Enhancement of genetic transformation efficiency in indica rice cultivar IR64 by vacuum infiltration and exogenous application of polyamines
Oryza sativa ssp. indica is not amenable to genetic modifications owing to its recalcitrant nature, rendering it difficult to transform and recover...
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Direct regeneration and genetic transformation studies in Hemidesmus indicus (L.) R. Br. (Indian Sarsaparilla)
Hemidesmus indicus L. R. Br. is a rare and endangered plant exhibits potent medicinal properties explored for the presence of flavoring compound in...
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Deciphering the role of MIR169d:NF-YA2 module under individual as well as combined drought and heat stress in Arabidopsis
Plants are often subjected to a combination of abiotic stresses under natural environmental conditions. The response of plants to combined stresses...
