Abstract
Key message
The first in-depth characterization of a subfamily III Snakin/GASA member was performed providing experimental evidence on promoter activity and subcellular localization and unveiling a role of potato Snakin-3 in defense
Abstract
Snakin/GASA proteins share 12 cysteines in conserved positions in the C-terminal region. Most of them were involved in different aspects of plant growth and development, while a small number of these peptides were reported to have antimicrobial activity or participate in abiotic stress tolerance. In potato, 18 Snakin/GASA genes were identified and classified into three groups based on phylogenetic analysis. Snakin-1 and Snakin-2 are members of subfamilies I and II, respectively, and were reported to be implicated not only in defense against pathogens but also in plant development. In this work, we present the first in-depth characterization of Snakin-3, a member of the subfamily III within the Snakin/GASA gene family of potato. Transient co-expression of Snakin-3 fused to the green fluorescent protein and organelle markers revealed that it is located in the endoplasmic reticulum. Furthermore, expression analyses via pSnakin-3::GUS transgenic plants showed GUS staining mainly in roots and vascular tissues of the stem. Moreover, GUS expression levels were increased after inoculation with Pseudomonas syringae pv. tabaci or Pectobacterium carotovorum subsp. carotovorum and also after auxin treatment mainly in roots and stems. To gain further insights into the function of Snakin-3 in planta, potato overexpressing lines were challenged against P. carotovorum subsp. carotovorum showing enhanced tolerance to this bacterial pathogen. In sum, here we report the first functional characterization of a Snakin/GASA gene from subfamily III in Solanaceae. Our findings provide experimental evidence on promoter activity and subcellular localization and reveal a role of potato Snakin-3 in plant defense.
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Data availability
The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.
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Acknowledgements
The authors thank Valeria Beracochea, Florencia Olivari, Laura Ramos, Matías Rodriguez, Ignacio Tevez, and Agustín Montenegro for their excellent technical assistance in the production and maintenance of transgenic potato plants. Microscopic analyses were performed at Laboratorio Integral de Microscopía, CICVyA, INTA.
Funding
This work was supported by PICT-2016-1444 from Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT) and by projects 2019-PD-E6-I116-001 and 2019-PD-E4-I085-001 from Instituto Nacional de Tecnología Agropecuaria (INTA).
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Nahirñak Vanesa participated in the design of the study, carried out the molecular techniques, and drafted the manuscript. Almasia Natalia Ines conducted experiments and helped to draft the manuscript. Lia Veronica Viviana contributed to data interpretation and contribute to writing the manuscript. Hopp Horacio Esteban assisted in the interpretation of the results and contributed to writing the manuscript revising it critically. Vazquez Rovere Cecilia conceived and coordinated the study, contributed to the work by the discussion of the data, and helped draft the manuscript. All authors read and approved the final manuscript.
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Communicated by Leandro Peña.
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Nahirñak, V., Almasia, N.I., Lia, V.V. et al. Unveiling the defensive role of Snakin-3, a member of the subfamily III of Snakin/GASA peptides in potatoes. Plant Cell Rep 43, 47 (2024). https://doi.org/10.1007/s00299-023-03108-4
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DOI: https://doi.org/10.1007/s00299-023-03108-4