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Flow Cytometry: The Glass Is Half Full
Accompanied by a historical perspective of the field of cytometry, this introductory chapter provides a broad view of what flow cytometry can do;...
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Laser Sources for Traditional and Spectral Flow Cytometry
Lasers for light scattering measurement and fluorescence excitation are essential components of all flow cytometers. Flow cytometers now typically...
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Spectral Flow Cytometry Analysis of Resident Tissue Macrophages
Spectral flow cytometry improves flow cytometry panels by resolving the full emission spectra of individual fluorophores, allowing greater...
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Panel Design and Optimization for Full Spectrum Flow Cytometry
Technological advancements in fluorescence flow cytometry and an ever-expanding understanding of the complexity of the immune system have led to the...
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Shedding Light on Intracellular Proteins using Flow Cytometry
Intracellular protein abundance is routinely measured in mammalian cells using population-based techniques such as western blotting which fail to...
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Multiparametric Analysis of Apoptosis by Flow Cytometry
Flow cytometry remains the most widely used method for detecting and quantifying apoptosis and related forms of cell death in mammalian cells. The...
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Isolation and Characterization of Testis Macrophages Using Flow Cytometry
Testis-resident macrophages are first responders of the innate immune system against pathogens. They also exert day-to-day functions that are poorly...
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Isolation and Flow Cytometry Analysis of Intestinal Macrophages
The intestinal macrophageIntestinal macrophages pool represents the largest population of macrophages present within the body. Nevertheless, flow...
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CytoPipeline and CytoPipelineGUI: a Bioconductor R package suite for building and visualizing automated pre-processing pipelines for flow cytometry data
BackgroundWith the increase of the dimensionality in flow cytometry data over the past years, there is a growing need to replace or complement...
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Quantification of Phagocytosis Using Flow Cytometry
Phagocytosis is relevant for many research fields and is often measured as a functional outcome. However, accurate quantification can be challenging,...
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Non-Conventional Flow Cytometry
This chapter briefly discusses the main technologies other than the traditional Flow Cytometry, i.e., (1) Imaging Flow Cytometry (IMC), (2) Spectral...
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Assaying Mitochondrial Function by Multiparametric Flow Cytometry
Flow cytometry has been a vital tool in cell biology for decades based on its versatile ability to detect and quantifiably measure both physical and...
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Quantifying Golgi Apparatus Fragmentation Using Imaging Flow Cytometry
Unlike the common conception of the Golgi apparatus as a static organelle, it is, in fact, a dynamic structure, as well as a sensitive sensor for the...
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Isolation and Flow Cytometry Analysis of Macrophages from White Adipose Tissue
Macrophages are one of the prominent leukocyte populations in white adipose tissue (WAT) and play an important role during WAT homeostasis and...
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The Application of Bacteriophage and Photoacoustic Flow Cytometry in Bacterial Identification
Infection with resistant bacteria has become an ever-increasing problem in modern medical practice. Bacteremia is a serious and potentially lethal...
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Use of flow cytometry method to detect contaminations of platelet suspensions
In this study, it was aimed to investigate bacterial contamination in apheresis platelet suspensions (APS) by automated blood culture system and flow...
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Imaging Flow Cytometry of Multi-Nuclearity
Multi-nuclearity is a common feature for cells in different cancers. Also, analysis of multi-nuclearity in cultured cells is widely used for...
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Isolation and Flow Cytometry Analysis of Macrophages from the Kidney
Renal macrophages help maintain homeostasis, participate in tissue injury and repair, and play a vital role in immune surveillance [1–3]. Kidney...
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Insights into the enumeration of mixtures of probiotic bacteria by flow cytometry
The use of flow cytometry to enumerate microorganisms is gaining traction over the traditional plate count technique on the basis of superior...
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Clinical Applications of Flow Cytometry in Cancer Immunotherapies: From Diagnosis to Treatments
The scope of flow cytometry is rapidly expanding in the diagnosis of various cancers, and it is being used routinely as an aid in classifying...