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Sodium acetate promotes the production of biomass and intracellular biochemical components by Micractinium reisseri FM1 under batch and fed-batch cultivation
Carbon source supply and feeding mode play an important effect on the growth and metabolite accumulation of microalgae. In order to investigate the...
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Diffusion-driven fed-batch fermentation in perforated ring flasks
PurposeSimultaneous membrane-based feeding and monitoring of the oxygen transfer rate shall be introduced to the newly established perforated ring...
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Optimized fed-batch cultivation of Rhodotorula toruloides in a bubble column bioreactor progressed the β-carotene production from corn steep liquor
In this work, a new isolate yeast, namely Rhodotorula toruloides KP324973, was examined for β -carotene production from corn steep liquor (CSL) as a...
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Progress in fed-batch culture for recombinant protein production in CHO cells
AbstractNearly 80% of the approved human therapeutic antibodies are produced by Chinese Hamster Ovary (CHO) cells. To achieve better cell growth and...
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β-glucosidase production by recombinant Pichia pastoris strain Y1433 under optimal feed profiles of fed-batch cultivation
Pichia pastoris , a methylotrophic yeast, is known to be an efficient host for heterologous proteins production. In this study, a recombinant P....
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Enhanced Production of Gamma-Aminobutyric Acid (GABA) from Lactobacillus futsaii CS3 Using Agri-Food Industries By-Products Under Batch and Fed-Batch Fermentation
Gamma-aminobutyric acid (GABA) has diverse physiological functions, but its production by lactic acid bacteria is costly due to the culture medium....
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A strategic media engineering and fed-batch approach enhances violacein production by Chromobacterium violaceum MTCC2656
Violacein, a therapeutic pigment synthesised naturally in specific bacterial systems, is commercially unexplored owing to low titers. The current...
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Medium composition and aeration to high (R,R)-2,3-butanediol and acetoin production by Paenibacillus polymyxa in fed-batch mode
Concerning the potential application of the optically active isomer (R,R)-2,3-butanediol, and its production by a non-pathogenic bacterium Paenibacillu...
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Production of ginsenoside compound K from American ginseng extract by fed-batch culture of Aspergillus tubingensis
Compound K (C-K), one of the most bioactive ginsenoside, is produced by hydrolyzing the glycoside moieties of protopanaxadiol (PPD)-type glycosylated...
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Development of a fed-batch fermentation with stepwise aeration to enhance docosahexaenoic acid and carotenoid content in Aurantiochytrium sp. FIKU018
Aurantiochytrium has been recommended as an alternative source for docosahexaenoic acid (DHA) since it accumulates DHA more than 50% of the...
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Squalene production under oxygen limitation by Schizochytrium sp. S31 in different cultivation systems
AbstractThe triterpene squalene is widely used in the food, cosmetics and pharmaceutical industries due to its antioxidant, antistatic and...
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Evaluation of different glycerol fed-batch strategies in a lab-scale bioreactor for the improved production of a novel engineered β-fructofuranosidase enzyme in Pichia pastoris
The β-fructofuranosidase enzyme from Aspergillus niger has been extensively used to commercially produce fructooligosaccharides from sucrose. In this...
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Limits to biomass productivity during fed-batch cultivation of Laminaria saccharina female gametophyte cells in a stirred-tank photobioreactor
This study compared the growth of Laminaria saccharina female gametophyte filamentous cell suspension cultures in a stirred-tank photobioreactor...
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High-cell-density cultivation of Vibrio natriegens in a low-chloride chemically defined medium
AbstractVibrio natriegens is a halophilic bacterium with the fastest generation time of non-pathogenic bacteria reported so far. It therefore has...
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Unveiling the potential of specific growth rate control in fed-batch fermentation: bridging the gap between product quantity and quality
During the epoch of sustainable development, leveraging cellular systems for production of diverse chemicals via fermentation has garnered attention....
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Thermal Analysis of Conventional and Extractive Fed-Batch Ethanol Fermentation at Different Temperatures
Very high gravity (VHG) ethanol fermentation at low temperatures can be a way to obtain wines with high ethanol content (> 15 °GL). The production of...
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Use of ambr®250 to assess mucic acid production in fed-batch cultures of a marine Trichoderma sp. D-221704
Mucic acid, a diacid with potential use in the food, cosmetic, chemical and pharmaceutical industries, can be produced by microbial conversion of...
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High-cell-density fed-batch cultivations of Vibrio natriegens
ObjectivesWith generation times of less than 10 min under optimal conditions, the halophilic Vibrio natriegens is the fastest growing non-pathogenic...
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Expression of Bacillus amyloliquefaciens transglutaminase in recombinant E. coli under the control of a bicistronic plasmid system in DO-stat fed-batch bioreactor cultivations
We studied the expression of Bacillus amyloliquefaciens transglutaminase cloned in Escherichia coli BL21(DE3)pLysS harboring the plasmid...
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Investigation of the robustness of Cupriavidus necator engineered strains during fed-batch cultures
It is of major interest to ensure stable and performant microbial bioprocesses, therefore maintaining high strain robustness is one of the major...