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Definitive Endoderm from EpiSC Aggregates in Matrigel
This chapter describes the protocol to derive definitive endoderm cells from epiblast stem cells (EpiSCs) via a process analogous to gastrulation in...
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Stability and biosafety of human epidermal stem cell for wound repair: preclinical evaluation
BackgroundCell therapy is a key technology to prevent sacrificing normal skin. Although some studies have shown the promise of human epidermal stem...
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Establishment of Mouse Epiblast Stem Cells
Epiblast stem cells are made from the epiblast of mouse post-implantation embryo. They have been critical in the understanding of mammalian...
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Grafting of Epiblast Stem Cell into the Epiblast and Whole-Embryo Imaging to Unveil Lineage Competence
Here we describe a method to engraft epiblast stem cells (EpiSC) into the epiblast of gastrulation-stage mouse embryo to test the lineage propensity...
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Complete Transcriptome Analysis by 5′-End Single-Cell RNA-Seq with Random Priming
Single-cell transcriptome analysis reveals heterogeneous cell types in complex tissues and leads to unexpected biological findings when compared to...
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c-Jun as a one-way valve at the naive to primed interface
Backgroundc-Jun is a proto-oncogene functioning as a transcription factor to activate gene expression under many physiological and pathological...
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Gastrulation: Its Principles and Variations
As epiblast cells initiate development into various somatic cells, they undergo a large-scale reorganization, called gastrulation. The gastrulation...
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Different Types of Pluripotent Stem Cells Represent Different Developmental Stages
Pluripotent stem cell lines established from early-stage embryos of mammals or other species represent the embryonic stages before the initiation of...
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The Epiblast and Pluripotent Stem Cell Lines
All somatic cells develop from the epiblast, which occupies the upper layer of two-layered embryos and in most mammals is formed after the...
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Epiblast Stem Cells Methods and Protocols
This book aims to deliver the most up-to-date protocols in using epiblast stem cells (EpiSC) to answer critical questions on mammalian development....
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Differentiation of EpiLCs on Micropatterned Substrates Generated by Micro-Contact Printing
During the last decades, signaling pathways responsible for the initiation of gastrulation in mammalian embryos have been identified. However, the...
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Exploring Chromatin Accessibility in Mouse Epiblast Stem Cells with ATAC-Seq
The assay for transposase-accessible chromatin using sequencing (ATAC-seq) is used to identify open chromatin regions in cells. This can be used to...
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Esrrb guides naive pluripotent cells through the formative transcriptional programme
During embryonic development, naive pluripotent epiblast cells transit to a formative state. The formative epiblast cells form a polarized...
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3D Enhancer–promoter networks provide predictive features for gene expression and coregulation in early embryonic lineages
Mammalian embryogenesis commences with two pivotal and binary cell fate decisions that give rise to three essential lineages: the trophectoderm, the...
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Transformation of Pluripotency States during Morphogenesis of Mouse and Human Epiblast
AbstractThe pluripotent status of a cell in vivo is spatio-temporally regulated within embryogenesis and is determined by the processes of...
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NETISCE: a network-based tool for cell fate reprogramming
The search for effective therapeutic targets in fields like regenerative medicine and cancer research has generated interest in cell fate...
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Derivation of Maternal Epiblast Stem Cells from Haploid Embryos
Pluripotent cells in the inner cell mass (ICM) or epiblast of mammalian embryos exhibit the capacity to differentiate into all cells represented in...
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Derivation of Naïve Human Embryonic Stem Cells Using a CHK1 Inhibitor
Embryonic development is a continuum in vivo . Transcriptional analysis can separate established human embryonic stem cells (hESC) into at least four...
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Allele-specific RNA-seq expression profiling of imprinted genes in mouse isogenic pluripotent states
BackgroundGenomic imprinting, resulting in parent-of-origin specific gene expression, plays a critical role in mammalian development. Here, we apply...
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Derivation of stable embryonic stem cell-like, but transcriptionally heterogenous, induced pluripotent stem cells from non-permissive mouse strains
Genetic background is known to play a role in the ability to derive pluripotent, embryonic stem cells (ESC), a trait referred to as permissiveness....
