SpringerLink
Log in

Epiblast Stem Cells

Methods and Protocols

  • Book
  • © 2022

Overview

Editors:
  1. Pierre Osteil

    1. Swiss Cancer Research Institute, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2490)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 99.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 129.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free ship** worldwide - see info
Hardcover Book USD 179.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free ship** worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

About this book

This book aims to deliver the most up-to-date protocols in using epiblast stem cells (EpiSC) to answer critical questions on mammalian development. Beginning with a section on the spectrum of mouse primed pluripotent stem cells, the volume continues with chapters on toolkits to characterize and study primed stem cells, as well as primed stem cells to engineer gastrulation models. Written for the highly successful Method in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Epiblast Stem Cells: Methods and Protocols serves as an ideal guide to overcome the reproducibility problem by gathering detailed state-of-the-art protocols to perform comprehensive analyses, enabling the reader to opt for the appropriate cell type to model the epiblast.

Similar content being viewed by others

Keywords

Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. The Spectrum of Mouse Primed Pluripotent Stem Cells

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Establishment of Mouse Epiblast Stem Cells

      • Pierre Osteil
      Pages 3-9

    3. L-Proline Supplementation Drives Self-Renewing Mouse Embryonic Stem Cells to a Partially Primed Pluripotent State: The Early Primitive Ectoderm-Like Cell

      • Hannah J. Glover, Rachel A. Shparberg, Michael B. Morris
      Pages 11-24

    4. Generation of Epiblast-Like Cells

      • Federica Cermola, Eduardo J. Patriarca, Gabriella Minchiotti
      Pages 25-36

  3. Toolkits to Characterize and Study Primed Stem Cells

    1. Front Matter

      Pages 37-37
      Download chapter PDF

    2. Identification and Visualization of Protein Expression in Whole Mouse Embryos by Immunofluorescence

      • V. Pragathi Masamsetti, Patrick P. L. Tam
      Pages 39-45

    3. Small Interfering RNA (siRNA) Transfection in Epiblast Stem Cells

      • Georgia R. Kafer
      Pages 47-55

    4. Measuring Endocytosis and Endosomal Uptake at Single Cell Resolution

      • Carolyn Sangokoya
      Pages 57-67

    5. 3D Immunofluorescent Image Colocalization Quantification in Mouse Epiblast Stem Cells

      • Joshua G. Dierolf, Andrew J. Watson, Dean H. Betts
      Pages 69-79

    6. Flow Cytometric Characterization of Pluripotent Cell Protein Markers in Naïve, Formative, and Primed Pluripotent Stem Cells

      • Joshua G. Dierolf, Kristin Chadwick, Courtney R. Brooks, Andrew J. Watson, Dean H. Betts
      Pages 81-92

    7. Exploring Chromatin Accessibility in Mouse Epiblast Stem Cells with ATAC-Seq

      • Nazmus Salehin, Nicole Santucci, Pierre Osteil, Patrick P. L. Tam
      Pages 93-100

    8. A Reproducible and Dynamic Workflow for Analysis and Annotation of scRNA-Seq Data

      • Nader Aryamanesh
      Pages 101-140

    9. Complete Transcriptome Analysis by 5′-End Single-Cell RNA-Seq with Random Priming

      • Tsukasa Kouno, Piero Carninci, Jay W. Shin
      Pages 141-156

    10. Global Proteomic Profiling of Embryonic Stem Cells Using iTRAQ Isobaric Tags with LC-MS/MS Quantification

      • Aseel Sharaireh, Anna L. Tierney, Richard D. Unwin
      Pages 157-177

    11. Comprehensive and Comparative Structural Glycome Analysis in Mouse Epiblast-like Cells

      • Federico Pecori, Hisatoshi Hanamatsu, Jun-ichi Furukawa, Shoko Nishihara
      Pages 179-193

  4. Primed Stem Cells to Engineer Gastrulation Model

    1. Front Matter

      Pages 195-195
      Download chapter PDF

    2. Generation of Gastruloids from Epiblast-Like Cells

      • Federica Cermola, Eduardo J. Patriarca, Gabriella Minchiotti
      Pages 197-204

    3. Definitive Endoderm from EpiSC Aggregates in Matrigel

      • Hisato Kondoh, Mai Fujii
      Pages 205-212

    4. In Vitro Differentiation of Murine Embryonic Stem Cells (ESCs) into Primordial Germ Cell-like Cells (PGCLCs)

      • Han-Pin Pui, Qiaolin Deng
      Pages 213-233
Back to top

Editors and Affiliations

  • Swiss Cancer Research Institute, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland

    Pierre Osteil

Bibliographic Information

  • Book Title: Epiblast Stem Cells

  • Book Subtitle: Methods and Protocols

  • Editors: Pierre Osteil

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2281-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-2280-3Published: 30 April 2022

  • Softcover ISBN: 978-1-0716-2283-4Published: 30 April 2023

  • eBook ISBN: 978-1-0716-2281-0Published: 29 April 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 303

  • Number of Illustrations: 9 b/w illustrations, 62 illustrations in colour

  • Topics: Stem Cells, Developmental Biology

Publish with us

Policies and ethics

Back to top

Search

Navigation