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Inducible Expression of dsRNA in Escherichia coli
Double-stranded RNA (dsRNA) is a valuable tool for reverse genetics research and gene silencing applications. It is also an important management...
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Identification of Mycoviruses by dsRNA Extraction
Mycoviruses exist in all major groups of fungi. With the continuous development of science and technology, the methods of studying viruses are...
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Rapid Purification of dsRNA Using Micro-Spin Cellulose Column
Double-stranded RNA (dsRNA) is the replication intermediates of all RNA viruses. Purification and analysis of the profile and sequence of dsRNA is...
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Detection of dsRNA by Acridine Orange Staining
Acridine orange is a nucleic acid binding dye that emits green fluorescence when bound to double-stranded DNA or RNA and red fluorescence when bound...
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Isolation of dsRNA from Plants by Cellulose Chromatography
As the constitutive molecules of double-stranded RNA (dsRNA) virus genomes and replicative intermediates of single-stranded RNA (ssRNA) viruses, the...
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Detection of dsRNA with Fluorescence In Situ Hybridization (FISH)
Fluorescence in situ hybridization (FISH) is a powerful technique used for detecting and localizing specific nucleic acid sequences in cells or...
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Application of dsRNA in the Pine Wood Nematode, Bursaphelenchus xylophilus
The pine wood nematode Bursaphelenchus xylophilus is one of the most destructive invasive species worldwide, causing the wilting and eventual death...
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Nanoparticle-mediated dsRNA delivery for precision insect pest control: a comprehensive review
Nanoparticle-based delivery systems have emerged as powerful tools in the field of pest management, offering precise and effective means of...
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Exogenous delivery of dsRNA for management of mungbean yellow mosaic virus on blackgram
Main conclusionExogenous application of dsRNA molecules targeting MYMV genes offers a promising approach to effectively mitigate yellow mosaic...
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In Vivo Production of dsRNA Using Bacteriophage ϕ6 in Pseudomonas syringae Cit7 Cells
RNA interference (RNAi), also known as post-transcriptional gene silencing (PTGS), is one of the emerging genetic engineering techniques to...
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Application of dsRNA for Fungi Disease Management Sclerotinia sclerotiorum and Botrytis cinerea
Sclerotinia sclerotiorum and Botrytis cinerea are two closely related necrotrophic plant pathogenic fungi that have garnered significant attention...
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Double-stranded RNA (dsRNA) technology to control forest insect pests and fungal pathogens: challenges and opportunities
Climate change alters the seasonal synchronization between plants and respective pests plus pathogens. The geographical infiltration helps to shift...
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Kaolinite nanoclay-shielded dsRNA drenching for management of Globodera pallida: An environmentally friendly pest management approach
Globodera pallida , an obligate sedentary endoparasite, is a major economic pest that causes substantial potato yield losses. This research aimed to...
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Subcellular Colocalization Assay of Host Factors with Viral Replication Complex in the dsRNA Reporter Nicotiana benthamiana
As obligate pathogens, plant viruses co-opt several host factors for viral replication. Double-stranded RNA (dsRNA) plays important roles in plants,...
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Exogenous dsRNA trigger RNAi in Venturia inaequalis resulting in down regulation of target genes and growth reduction
BackgroundVenturia inaequalis is an apple scab causing fungal pathogen. It is a highly contagious and destructive pathogen which rapidly spreads...
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Topical application of dsRNA for plant virus control: a review
RNA interference (RNAi) is a mechanism in which dsRNA is processed by the cell machinery mediating post-transcriptional or transcriptional gene...
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Drosophila X virus-like particles as efficient dsRNA carriers for improved RNAi against the invasive species, Drosophila suzukii
RNA interference (RNAi) has been exploited as a promising strategy for pest control and also has great potential against the spotted wing drosophila...
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Control of cucumber mosaic virus in rockmelon using dsRNA-mediated silencing of coat protein and movement protein genes with no deleterious effect on plant phenotype
Rockmelon is a popular tropical fruit with high nutritional value. Cucumber mosaic virus (CMV), an aphid-transmitted virus, causes severe damage to...
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Size variations of mesoporous silica nanoparticle control uptake efficiency and delivery of AC2-derived dsRNA for protection against tomato leaf curl New Delhi virus
Key messageWe report the size dependent uptake of dsRNA loaded MSNPs into the leaves and roots of
Nicotiana benthamiana plants and accessed for their...
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Cellular origins of dsRNA, their recognition and consequences
Double-stranded RNA (dsRNA) is associated with most viral infections — it either constitutes the viral genome (in the case of dsRNA viruses) or is...
