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Comparison of three AUC techniques for the determination of the loading status and capsid titer of AAVs
Due to the rise of adeno-associated viruses (AAVs) as gene therapy delivery vectors, boundary sedimentation velocity analytical ultracentrifugation...
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Transcriptome analysis revealed that Arabidopsis model plant invokes the activation of heat shock proteins and ER stress response against cesium stress
Cesium (Cs) toxicity has deleterious effects on plant growth and development. However, the molecular mechanism of the toxic effect of Cs on plants...
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Cesium adsorption ability of charcoal made from Japanese cedar and Japanese oak wood: effect of Fe3+-addition to starting materials
To investigate the effect of Fe-addition on the Cs + -adsorption ability (Cs + -AA) of woody charcoal, charcoal samples were prepared from Japanese cedar...
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Density Gradient-Based Nucleic Acid Isolation
Nucleic acid (DNA/RNA) isolation has a wide range of applications, right from research purposes to applications such as food development,...
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Propagation of viral genomes by replicating ammonia-oxidising archaea during soil nitrification
Ammonia-oxidising archaea (AOA) are a ubiquitous component of microbial communities and dominate the first stage of nitrification in some soils....
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Dominance of phage particles carrying antibiotic resistance genes in the viromes of retail food sources
The growth of antibiotic resistance has stimulated interest in understanding the mechanisms by which antibiotic resistance genes (ARG) are mobilized....
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Stem Cells as Target for Prostate cancer Therapy: Opportunities and Challenges
Cancer stem cells (CSCs) and cells in a cancer stem cell-like (CSCL) state have proven to be responsible for tumor initiation, growth, and relapse in...
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A Higher Stomatal Aperture is Associated with the Growth Promotion of Centipedegrass (Eremochloa ophiuroides (Munro) Hack.) Under High Concentrations of Ammonium
The key to improving the efficiency by which acid-soil-growing plants utilize nitrogen is to improve their efficiency of utilizing ammonium. The...
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Optimizing high-throughput viral vector characterization with density gradient equilibrium analytical ultracentrifugation
Viral vector-based gene therapies and vaccines require accurate characterization of capsid species. The current gold standard for assessing capsid...
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DNA-, RNA-, and Protein-Based Stable-Isotope Probing for High-Throughput Biomarker Analysis of Active Microorganisms
Stable-isotope probing (SIP) enables researchers to target active populations within complex microbial communities, which is achieved by providing...
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Influence of Cobalt, Cesium, and Combined Stresses on Physiological Parameters and Gene Expression of Two Hullless Barley Varieties
AbstractThis study investigated the impact of cobalt and cesium stresses on two hulless barley varieties through a hydroponic experiment. The plants...
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Fast Identification of In Vivo Protein Phosphorylation Events Using Transient Expression in Leaf Mesophyll Protoplasts and Phos-tagTM SDS-PAGE
Phosphorylation/dephosphorylation is a key posttranslational mechanism for signal transduction and amplification. Several techniques exist for...
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Hydrodynamic characterization of a vesicular stomatitis virus-based oncolytic virus using analytical ultracentrifugation
Determination of the size, density, and mass of viral particles can provide valuable information to support process and formulation studies in...
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A new UltraScan module for the characterization and quantification of analytical buoyant density equilibrium experiments to determine AAV capsid loading
A method for characterizing and quantifying peaks formed in an analytical buoyant density equilibrium (ABDE) experiment is presented. An algorithm is...
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Alkaline taste sensation through the alkaliphile chloride channel in Drosophila
The sense of taste is an important sentinel governing what should or should not be ingested by an animal, with high pH sensation playing a critical...
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Archaella Isolation
Swimming archaea are propelled by a filamentous structure called the archaellum. The first step for the structural characterization of this filament...
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Imaging of Mucin Networks with Atomic Force Microscopy
Mucin networks serve as the structural scaffold of mucus and play a significant role in determining its biophysical properties. Thus, characterizing...
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Propagation, Purification, and Characterization of Bacteriophages for Phage Therapy
Phage therapy is an alternative approach to combat bacterial infections. In this approach, bacteriophages are used as antimicrobial agents due to...
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A Metagenomics Approach to Enumerate Bacteriophages in a Food Niche
Dairy fermentation relies on the activity of starter cultures composed primarily of lactic acid bacteria such as Lactococcus and Streptococcus...
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The influence of cations on α-lactalbumin amyloid aggregation
There is limited knowledge regarding α-lactalbumin amyloid aggregation and its mechanism. We examined the formation of α-lactalbumin amyloid fibrils...
