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Screening of P-35S, P-FMV, and T-NOS genetic elements in microwave-treated genetically modified cereal flours
BackgroundReliable and efficient methods for detecting genetically modified organisms (GMOs) in unprocessed and processed food will be essential for...
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35S promoter-driven transgenes are variably expressed in different organs of Arabidopsis thaliana and in response to abiotic stress
The cauliflower mosaic virus (CaMV) 35S promoter is known as the most frequently used promoter in plant biotechnology. Although it is widely...
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Aspartic protease inhibitor enhances resistance to potato virus Y and A in transgenic potato plants
BackgroundViruses are the major threat to commercial potato ( Solanum tuberosum ) production worldwide. Because viral genomes only encode a small...
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Genetic modification of crop plants with ribosome-inactivating protein genes for enhanced resistance to pathogens and pests
Genetic engineering has emerged as an attractive strategy for incorporating resistance in plants against diverse pathogens and has been largely...
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A high-efficient and naked-eye visible CRISPR/Cas9 system in Arabidopsis
Main conclusionIntroducing 35S - dsRED2 into the Cas9 vector which expresses naked-eye visible dsRED2 greatly facilitates the genetic screening, and...
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Transiently Induce RNA Silencing in Plants Using a Tobacco Necrosis Virus A (TNV-A)-Based dsRNA Production System
Transgenic expression of hairpin RNA or artificial microRNA is widely used for genetic studies in plant science. However, induction of RNA silencing...
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Overexpression of R2R3-MYB IbMYB1a induces anthocyanin pigmentation in soybean cotyledon
Key messageThis is the first report showing anthocyanin accumulation in the soybean cotyledon via genetic transformation of a single gene .
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Viral-based expression cassettes ensure high level production of recombinant green fluorescent protein (GFP) in sweet basil (Ocimum basilicum) plants
Sweet basil ( Ocimum basilicum ) is a widespread edible aromatic herb used in many countries for medicinal and culinary purposes. Here, we disclose...
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Identification of a sugarcane bacilliform virus promoter that is activated by drought stress in plants
Sugarcane ( Saccharum spp.) is an important sugar and biofuel crop in the world. It is frequently subjected to drought stress, thus causing...
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An Agrobacterium-mediated transient expression method contributes to functional analysis of a transcription factor and potential application of gene editing in Chenopodium quinoa
Key messageAn efficient Agrobacterium -mediated transient expression method was developed, which contributed to the functional characterization of...
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Anti-phytoviral Activity of Carvacrol vis-a-vis Cauliflower Mosaic Virus (CaMV)
Viruses are causative agents of infections in plants which are found worldwide and cause a serious threat to modern agriculture. Since the effect of...
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Development of potent promoters that drive the efficient expression of genes in apple protoplasts
Protoplast transient expression is a powerful strategy for gene functional characterization, especially in biochemical mechanism studies. We herein...
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Potential of Wheat dwarf virus (Geminiviridae: Mastrevirus) Truncated Promoter for Improvement of Transgene Expression in Rice
Wheat dwarf virus (WDV) is a monocotyledonous plant-infecting virus that causes economical loss to cereal cultivations annually. The objective of...
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Glyphosate-resistant Brassica juncea (oilseed mustard) transgenics for possible control of root parasite Orobanche aegyptiaca and conservation agriculture
Brassica juncea (mustard) is a major oilseed crop of India grown in around five to six million hectares of land under low moisture availability. In...
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Functional assessment of AtPAP17; encoding a purple acid phosphatase involved in phosphate metabolism in Arabidopsis thaliana
PurposePurple acid phosphatases (PAPs) includ the largest classes of non-specific plant acid phosphatases. Most characterized PAPs were found to play...
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Construction of viral-based expression vectors for high-level production of human interferon alpha 2b in plants
AbstractHuman interferon (hINF) alpha 2b is clinically important pharmaceutical product included in combinatory therapy against chronic hepatitis C...
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Orthogonal control of gene expression in plants using synthetic promoters and CRISPR-based transcription factors
BackgroundThe construction and application of synthetic genetic circuits is frequently improved if gene expression can be orthogonally controlled,...
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Synthetic sub-genomic transcript promoter from Horseradish Latent Virus (HRLV)
Main conclusionWe characterized an efficient chimeric sub-genomic transcript promoter from Horseradish Latent Virus, FHS4, active in both dicot and...
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Comprehensive analysis of the UDP-glucuronate decarboxylase (UXS) gene family in tobacco and functional characterization of NtUXS16 in Golgi apparatus in Arabidopsis
BackgroundUDP-glucuronate decarboxylase (also named UXS) converts UDP-glucuronic acid (UDP-GlcA) to UDP-xylose (UDP-Xyl) by decarboxylation of the...
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Overexpression of sucrose synthase enhances cellulose content in transgenic Populus deltoides Bartr. ex Marsh
Cellulose is the most abundant biopolymer with many industrial and domestic applications. Sucrose synthase (SuSy) enzyme plays an important role in...
