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A genome-wide CRISPR screen identifies BRD4 as a regulator of cardiomyocyte differentiation
Human induced pluripotent stem cell (hiPSC) to cardiomyocyte (CM) differentiation has reshaped approaches to studying cardiac development and...
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A CRISPR/Cas9 screen in embryonic stem cells reveals that Mdm2 regulates totipotency exit
During early embryonic development, the transition from totipotency to pluripotency is a fundamental and critical process for proper development....
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An in vitro CRISPR screen of cell-free DNA identifies apoptosis as the primary mediator of cell-free DNA release
Clinical circulating cell-free DNA (cfDNA) testing is now routine, however test accuracy remains limited. By understanding the life-cycle of cfDNA,...
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ShrinkCRISPR: a flexible method for differential fitness analysis of CRISPR-Cas9 screen data
BackgroundCRISPR screens provide large-scale assessment of cellular gene functions. Pooled libraries typically consist of several single guide RNAs...
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CRISPR screen identifies the role of RBBP8 in mediating unfolded protein response induced liver damage through regulating protein synthesis
Unfolded protein response (UPR) maintains the endoplasmic reticulum (ER) homeostasis, survival, and physiological function of mammalian cells....
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Efficient prioritization of CRISPR screen hits by accounting for targeting efficiency of guide RNA
BackgroundCRISPR-based screens are revolutionizing drug discovery as tools to identify genes whose ablation induces a phenotype of interest. For...
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Surfaceome CRISPR screen identifies OLFML3 as a rhinovirus-inducible IFN antagonist
BackgroundRhinoviruses (RVs) cause more than half of common colds and, in some cases, more severe diseases. Functional genomics analyses of RVs using...
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Genome-wide CRISPR screen for HSV-1 host factors reveals PAPSS1 contributes to heparan sulfate synthesis
Herpes simplex virus type 1 (HSV-1) is a ubiquitous pathogen that causes various diseases in humans, ranging from common mucocutaneous lesions to...
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SCEPTRE improves calibration and sensitivity in single-cell CRISPR screen analysis
Single-cell CRISPR screens are a promising biotechnology for map** regulatory elements to target genes at genome-wide scale. However, technical...
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Genome-wide CRISPR/Cas9 screen shows that loss of GET4 increases mitochondria-endoplasmic reticulum contact sites and is neuroprotective
Organelles form membrane contact sites between each other, allowing for the transfer of molecules and signals. Mitochondria-endoplasmic reticulum...
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Integrating genome-wide CRISPR screens and in silico drug profiling for targeted antidote development
Numerous toxins threaten humans, but specific antidotes are unavailable for most of them. Although CRISPR screening has aided the discovery of the...
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Genome Editing by CRISPR/Cas9 in Polyploids
CRISPR/Cas system has been widely used for genome editing in the past few years. Even though it has been performed in many polyploid species to date,...
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Genome-wide pooled CRISPR screening in neurospheres
Spheroid culture systems have allowed in vitro propagation of cells unable to grow in canonical cell culturing conditions, and may capture cellular...
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CRISPR Activation/Interference Screen to Identify Genetic Networks in HDAC-Inhibitor-Resistant Cells
Epigenetic alterations have been identified in various tumor types. In part, these alterations are mediated via increased histone deacetylase...
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Defining E3 ligase–substrate relationships through multiplex CRISPR screening
Specificity within the ubiquitin–proteasome system is primarily achieved through E3 ubiquitin ligases, but for many E3s their substrates—and in...
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Epigenetic-focused CRISPR/Cas9 screen identifies (absent, small, or homeotic)2-like protein (ASH2L) as a regulator of glioblastoma cell survival
BackgroundGlioblastoma is the most common and aggressive primary brain tumor with extremely poor prognosis, highlighting an urgent need for...
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CRISPR–Cas9 applications in T cells and adoptive T cell therapies
T cell immunity is central to contemporary cancer and autoimmune therapies, encompassing immune checkpoint blockade and adoptive T cell therapies....
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A Practical Guide for CRISPR-Cas9-Induced Mutations in Axolotls
Clustered regularly interspaced short palindromic repeats (CRISPR) is a powerful tool that enables editing of the axolotl genome. In this chapter, we...
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Compact CRISPR genetic screens enabled by improved guide RNA library cloning
CRISPR genome editing approaches theoretically enable researchers to define the function of each human gene in specific cell types, but challenges...
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Revolutionizing DNA repair research and cancer therapy with CRISPR–Cas screens
All organisms possess molecular mechanisms that govern DNA repair and associated DNA damage response (DDR) processes. Owing to their relevance to...
