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Genome Editing by CRISPR/Cas9 in Polyploids

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Polyploidy

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2545))

Abstract

CRISPR/Cas system has been widely used for genome editing in the past few years. Even though it has been performed in many polyploid species to date, its efficient accomplishment in these organisms is still a challenge. The presence of multiple homoeologous genes as targets for their editing requires more rigorous work and specific needs to assess successful genome editing. Here, we describe a general stepwise protocol to select target sites, design sgRNAs, indicate vector requirements, and screen CRISPR/Cas9-mediated genome editing in polyploid species.

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Acknowledgments

CSG was supported by a grant from the Spanish Ministries of Science and Innovation (MICINN, RTI2018-09309-A-I00). CMP was supported by a grant from the European Research Council (ERC-2014-StG 638134).

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Authors and Affiliations

  1. Departamento de Mejora Genética y Biotecnología, Instituto de Hortofruticultura Subtropical y Mediterránea (IHSM), Universidad de Málaga – Consejo Superior de Investigaciones Científicas, Departamento de Biología Molecular y Bioquímica, Facultad de Ciencias, UMA, Málaga, Spain

    Carlos Sánchez-Gómez, David Posé & Carmen Martín-Pizarro

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  1. Carlos Sánchez-Gómez
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  2. David Posé
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  3. Carmen Martín-Pizarro
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Correspondence to Carmen Martín-Pizarro .

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  1. Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium

    Yves Van de Peer

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Sánchez-Gómez, C., Posé, D., Martín-Pizarro, C. (2023). Genome Editing by CRISPR/Cas9 in Polyploids. In: Van de Peer, Y. (eds) Polyploidy. Methods in Molecular Biology, vol 2545. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2561-3_24

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  • DOI: https://doi.org/10.1007/978-1-0716-2561-3_24

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2560-6

  • Online ISBN: 978-1-0716-2561-3

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