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CRISPR/Cas9
CRISPR/Cas9 is the genome-editing technology that is most widely used around the world. Its widespread adoption is largely due to its simplicity and...
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Precision Genome Editing with CRISPR-Cas9
The CRISPR/Cas9 system is a revolutionary technology for genome editing that allows for precise and efficient modifications of DNA sequences. The...
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Callus-specific CRISPR/Cas9 system to increase heritable gene mutations in maize
Main conclusionA callus-specific CRISPR/Cas9 (CSC) system with Cas9 gene driven by the promoters of ZmCTA1 and ZmPLTP reduces somatic mutations and...
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An Efficient Vector-Based CRISPR/Cas9 System in Zebrafish Cell Line
The clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) system has been widely applied in animals as...
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Mechanisms of the Specificity of the CRISPR/Cas9 System in Genome Editing
AbstractThe CRISPR/Cas9 system, which was discovered recently, utilizes nucleases targeted by sequence complementarity and is originally intended to...
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Plant Breeding Using the CRISPR-Cas9 System for Food Security and Facing Climate Change
With the rapid expansion of the global population and the influence of climate change on agriculture, crops with higher yields and greater resistance...
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CRISPR/Cas9-based Genome Editing of Pseudomonas aeruginosa
Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 system has been developed as a robust genome engineering tool in a variety of...
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Genome editing of soybean inoculant using CRISPR/Cas9 system: enhancing agricultural sustainability
In modern agriculture, inoculants, consisting of various bacteria and fungi, are essential for promoting crop growth and sustainability while...
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Benefits of Using the CRISPR/Cas9 System for the Correction of Genetic Mutations
The discovery of the CRISPR/Cas9 gene scissors technology was a breakthrough in the development of gene therapy methods and attracted the attention...
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Application of CRISPR-Cas9 Technology in Fish
CRISPR/Cas9-based genome editing allows efficient and targeted enhancement of important aquaculture traits and will probably be the solution to the...
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CRISPR-Cas9-Mediated Genome Modifications in Zebrafish
CRISPR-Cas9 genome editing technology has been successfully applied to generate various genetic modifications in zebrafish. The CRISPR-Cas9 system,...
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Generating Mutant for a Stress-Responsive Gene in Rice Using CRISPR-Cas9 System
CRISPR/Cas9 system is one of the most often utilized engineering tools for genome editing in many organisms including crop plants and presents great...
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Targeted elimination of Vancomycin resistance gene vanA by CRISPR-Cas9 system
ObjectiveThe purpose of this study is to reduce the spread of the v anA gene by curing the vanA -harboring plasmid of vancomycin-resistant using the...
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Application of Repetitive Sequences in Fish Cell Depletion as a Target for the CRISPR/Cas9 System
Specific cell depletion is a common means to study the physiological function of cell lineages and tissue regeneration. However, 100% depletion is...
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Development of a Csy4-Processing TRV-Based CRISPR/Cas9 Genome Editing System in Nicotiana benthamiana
The CRISPR/Cas9 system is a site-specific genome editing tool that has been widely used in various plant species. The plant virus-based gRNA (guide...
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CRISPR-Cas9 System Mediated Genome Editing Technology: An Ultimate Tool to Enhance Abiotic Stress in Crop Plants
The drastic rise in the human population globally might uplift the issue of food scarcity in the coming few decades. This problem could affect the...
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Applications of CRISPR/Cas9 Technology in Ornamental Plants
Gene editing allows for precise editing and modification of specific target genes within an organism’s genome. The CRISPR/Cas9 technology has become...
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Revolutionizing Agriculture: Harnessing CRISPR/Cas9 for Crop Enhancement
Plant crops serve as essential sources of nutritional sustenance, supplying vital nutrients to human diets. However, their productivity and quality...
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CRISPR/Cas9 Vector Construction for Gene Knockout
This protocol outlines the construction of a plant transformation plasmid to express both the Cas9 nuclease and individual guide RNA (gRNA),...
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Genome Editing by CRISPR/Cas9 in Polyploids
CRISPR/Cas system has been widely used for genome editing in the past few years. Even though it has been performed in many polyploid species to date,...