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1. Production of 21-hydroxy-20-methyl-pregna-1,4-dien-3-one by modifying multiple genes in Mycolicibacterium

   Abstract

   C22 steroid drug intermediates are suitable for corticosteroids synthesis, and the production of C22 steroids is unsatisfactory due to the...

   Chenyang Yuan, Zhiguo Ma, ... Baoguo Zhang in Applied Microbiology and Biotechnology

   Article 02 February 2023
   

2. Current Trends and Perspectives in Microbial Bioconversions of Steroids

   The microbiological transformation of sterols is currently the technological basis for the industrial production of valuable steroid precursors, the...

   Marina V. Donova in Microbial Steroids

   Protocol 2023
   

3. Purification, characterization, and application of a high activity 3-ketosteroid-Δ¹-dehydrogenase from Mycobacterium neoaurum DSM 1381

   Δ ¹ -Dehydrogenation is one of the most important reactions for steroid drug modification. Numerous 3-ketosteroid-Δ ¹ -dehydrogenases (KstDs) catalyzing...

   Ruijie Zhang, Xuexia Xu, ... Baoguo Zhang in Applied Microbiology and Biotechnology

   Article 09 July 2019
   

4. Expression of Synthetic cyp102A1-LG23 Gene and Functional Analysis of Recombinant Cytochrome P450 BM3-LG23 in the Actinobacterium Mycolicibacterium smegmatis

   Abstract

   Cytochrome CYP102A1 (P450 BM3) of Priestia megaterium (bas. Bacillus megaterium ) has several unique functional features and thus provides an...

   Veronika Y. Poshekhontseva, Nikolai I. Strizhov, ... Marina V. Donova in Biochemistry (Moscow)

   Article 22 September 2023
   

5. Obtaining of 24-Norchol-4-ene-3,22-dione from Phytosterol with Mutants of Mycolicibacterium neoaurum

   Engineered mutants of Mycolicibacterium spp. are known producers of valuable steroid synthons with C19 or C22 skeleton. Here we describe a method for...

   Dmitry V. Dovbnya, Tanya V. Ivashina, ... Marina V. Donova in Microbial Steroids

   Protocol 2023
   

6. Enzyme Structure and Function

   This chapter provides an overview on the general architecture of enzymes, describes protein structure and the types of enzyme cofactors, and...

   Vlada B. Urlacher, Katja Koschorreck in Introduction to Enzyme Technology

   Chapter 2024
   

7. Phytosterol conversion into C9 non-hydroxylated derivatives through gene regulation in Mycobacterium fortuitum

   Abstract

   Androst-4-ene-3,17-dione (AD) and 22-hydroxy-23,24-bisnorchol-4-ene-3-one (4-HBC) are important drug intermediates that can be biosynthesized...

   **angcen Liu, Beiru He, ... Baoguo Zhang in Applied Microbiology and Biotechnology

   Article 13 October 2023
   

8. Identification and Characterization of Some Genes, Enzymes, and Metabolic Intermediates Belonging to the Bile Acid Aerobic Catabolic Pathway from Pseudomonas

   The study of the catabolic potential of microbial species isolated from different habitats has allowed the identification and characterization of...

   José M. Luengo, Elias R. Olivera in Microbial Steroids

   Protocol 2023
   

9. Creation and Functional Analysis of Mycolicibacterium smegmatis Recombinant Strains Carrying the Bacillary Cytochromes CYP106A1 and CYP106A2 Genes

   Mycolicibacterium smegmatis mc ² 155 has been genetically modified to be used as a platform for the expression of heterologous cytochrome P450...

   M. V. Karpov, V. M. Nikolaeva, ... M. V. Donova in Applied Biochemistry and Microbiology

   Article 16 December 2022
   

10. Biotransformation of Industrially Important Steroid Drug Precursors

    Steroids are known to act as wonder drugs. The production of steroidal hormones and drugs rests mainly on sterols of plant origin, but most of them...

    Girish B. Pendharkar, Tushar Banerjee, ... Bhushan L. Chaudhari in Industrial Microbiology and Biotechnology

    Chapter 2022
    

11. Model Setup and Procedures for Prediction of Enzyme Reaction Kinetics with QM-Only and QM:MM Approaches

    The enzyme-catalyzed reactions are traditionally studied with experimental kinetic assays. The modern theoretical modeling techniques provide a...

    Michał Glanowski, Sangita Kachhap, ... Maciej Szaleniec in Computational Methods for Estimating the Kinetic Parameters of Biological Systems

    Protocol 2022
    

12. Cultivation of Mycolicibacterium spp. Mutants in Miniaturized and High-Throughput Format to Characterize Their Growth, Phytosterol Conversion Ability, and Resistance to the Steroid Products

    This chapter describes methods for cultivation and characterization of the growth of Mycolicibacterium spp. mutants in a microbioreactor system in...

    Simone Balzer Le, Anna Nordborg, ... Håvard Sletta in Microbial Steroids

    Protocol 2023
    

13. The function of steroid terminal monooxygenase in cholesterol or phytosterol degradation by the Mycobacterium neoaurum NRRL B-3805

    Cholesterol and phytosterols can be served as the substrates for Actinobacteria to produce the precursors used in the synthesis of steroidal...

    Xuemei Li, Rui Zhang, ... Yanhe Ma in Systems Microbiology and Biomanufacturing

    Article 16 February 2023
    

14. Microbial 1,2-Dehydrogenation of 6 α-Methylhydrocortisone 11-Trifluoroacetate

    Transformation of 11-trifluoroacetate 6α-methylhydrocortisone (11-TFA MHC) by cells of the actinobacteria Arthrobacter (Nocardioides) was carried out...

    T. S. Savinova, A. Yu. Arinbasarova, ... N. V. Lukashev in Applied Biochemistry and Microbiology

    Article 10 December 2023
    

15. Microbial Degradation of Steroids

    Steroids are naturally found in humans, plants, fungi, and arthropods to implement several developmental functions. Steroids are also extensively...

    Shashi Kiran Misra, Kamla Pathak, Devender Pathak in Recent Advances in Microbial Degradation

    Chapter 2021
    

16. A general overview of the multifactorial adaptation to cold: biochemical mechanisms and strategies

    Cold environments are more frequent than people think. They include deep oceans, cold lakes, snow, permafrost, sea ice, glaciers, cold soils, cold...

    Ana Ramón, Adriana Esteves, ... Susana Castro-Sowinski in Brazilian Journal of Microbiology

    Article 21 July 2023
    

17. Anaerobic Biodegradation of Steroids

    Steroids, comprising sterols, steroid hormones, and bile acids, are ubiquitous and abundant in nature. Steroids are largely produced by eukaryotes;...

    Yin-Ru Chiang, Wael Ismail in Anaerobic Utilization of Hydrocarbons, Oils, and Lipids

    Reference work entry 2020
    

18. Identification of steroid C27 monooxygenase isoenzymes involved in sterol catabolism and stepwise pathway engineering of Mycobacterium neoaurum for improved androst-1,4-diene-3,17-dione production

    Cholesterol oxidase, steroid C27 monooxygenase and 3-ketosteroid-Δ ¹ -dehydrogenase are key enzymes involved in microbial catabolism of sterols. Here,...

    Minglong Shao, **an Zhang, ... Shangtian Yang in Journal of Industrial Microbiology & Biotechnology

    Article 21 February 2019
    

19. Crystallographic Studies of Steroid-Protein Interactions

    Steroid molecules have a wide range of function in eukaryotes, including the control and maintenance of membranes, hormonal control of transcription,...

    Arthur F. Monzingo in Direct Mechanisms in Cholesterol Modulation of Protein Function

    Chapter 2019
    

20. Site-directed mutagenesis under the direction of in silico protein docking modeling reveals the active site residues of 3-ketosteroid-Δ¹-dehydrogenase from Mycobacterium neoaurum

    3-Ketosteroid-Δ ¹ -dehydrogenases ( KsdD ) from Mycobacterium neoaurum could transform androst-4-ene-3,17-dione (AD) to androst-1,4-diene-3,17-dione....

    Ning Qin, Yanbing Shen, ... Min Wang in World Journal of Microbiology and Biotechnology

    Article 20 June 2017