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Spatially resolved epigenome sequencing via Tn5 transposition and deterministic DNA barcoding in tissue
Spatial epigenetic map** of tissues enables the study of gene regulation programs and cellular functions with the dependency on their local tissue...
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Nanobody-tethered transposition enables multifactorial chromatin profiling at single-cell resolution
Chromatin states are functionally defined by a complex combination of histone modifications, transcription factor binding, DNA accessibility and...
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Experimental Approaches to Study Somatic Transposition in Drosophila Using Whole-Genome DNA Sequencing
The extent of transposable element (TE) mobilization in different somatic tissues and throughout diverse species is not well understood. Somatic...
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A sensitive assay for scrutiny of Autographa californica multiple nucleopolyhedrovirus genes using CRISPR-Cas9
AbstractBaculoviruses have very large genomes and previous studies have demonstrated improvements in recombinant protein production and genome...
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Assay for Transposase-Accessible Chromatin Using Sequencing of Freshly Isolated Muscle Stem Cells
Actively transcribed genes harbor cis-regulatory modules with comparatively low nucleosome occupancy and few high-order structures (=“open...
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Uncontrolled transposition following RNAi loss causes hypermutation and antifungal drug resistance in clinical isolates of Cryptococcus neoformans
Cryptococcus neoformans infections cause approximately 15% of AIDS-related deaths owing to a combination of limited antifungal therapies and drug...
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First Biochemical Steps on Bacterial Transposition Pathways
Transposons are found in a wide variety of forms throughout the prokaryotic world where they actively contribute to the adaptive strategies of...
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A gold nanoparticles-based lateral flow assay utilizing baculovirus expressed recombinant nucleocapsid and receptor binding domain proteins for serodetection of IgG and IgM against SARS-CoV-2
Serological assays for SARS-CoV-2 are being utilized at an exponential rate for surveillance programs. This enterprise was designed to develop and...
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Laboratory validation of loop-mediated isothermal amplification (LAMP) assay for Limnoperna fortunei (Dunker 1857) detection
Among the invasive species known to occur in South America is the bivalve mollusc Limnoperna fortunei , whose presence is linked to several...
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How mouse RAG recombinase avoids DNA transposition
The RAG1-RAG2 recombinase (RAG) cleaves DNA to initiate V(D)J recombination, but RAG also belongs to the RNH-type transposase family. To learn how...
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Comparative genomics reveal unique markers to monitor by routine PCR assay bioinoculant of Sphingobium indicum B90A in hexachlorocyclohexane (HCH) contaminated soils
Bioinoculants of Sphingobium indicum B90A have been used to decontaminate hexachlorocyclohexane (HCH)-contaminated soils in the past. There is no...
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Chromatin accessibility profiling by ATAC-seq
The assay for transposase-accessible chromatin using sequencing (ATAC-seq) provides a simple and scalable way to detect the unique chromatin...
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Medulloblastoma and high-grade glioma organoids for drug screening, lineage tracing, co-culture and in vivo assay
Medulloblastoma and high-grade glioma represent the most aggressive and frequent lethal solid tumors affecting individuals during pediatric age....
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Targeted DNA integration in human cells without double-strand breaks using CRISPR-associated transposases
Conventional genome engineering with CRISPR–Cas9 creates double-strand breaks (DSBs) that lead to undesirable byproducts and reduce product purity....
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Genome-Wide Map** of Active Regulatory Elements Using ATAC-seq
Active cis-regulatory elements (cREs) in eukaryotes are characterized by nucleosomal depletion and, accordingly, higher accessibility. This property...
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A high-throughput screening assay based on automated microscopy for monitoring antibiotic susceptibility of Mycobacterium tuberculosis phenotypes
BackgroundEfficient high-throughput drug screening assays are necessary to enable the discovery of new anti-mycobacterial drugs. The purpose of our...
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Transgenic direct terminal repeats of Dissociation (Ds) produce chromosomal breakage in maize
Barbara McClintock recognized transposable elements originally by the movement of a site of chromosomal breakage, a genetic element called Dissociation ...
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ATAC-Seq Analysis of Accessible Chromatin: From Experimental Steps to Data Analysis
Accessible chromatin often represents gene regulatory elements, including promoters and enhancers, essential for gene expression. Assay for...
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Long terminal repeats (LTR) and transcription factors regulate PHRE1 and PHRE2 activity in Moso bamboo under heat stress
BackgroundLTR retrotransposons play a significant role in plant growth, genome evolution, and environmental stress response, but their regulatory...
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Development of activation-tagged gain-of-functional mutants in indica rice line (BPT 5204) for sheath blight resistance
BackgroundThe development of sheath blight (ShB) resistance varieties has been a challenge for scientists for long time in rice. Activation tagging...
