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Old and New Drugs for the Management of Bone Disorders in CKD
Disturbances in mineral and bone metabolism are common in patients with chronic kidney disease (CKD), especially those undergoing dialysis. Renal...
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Lipid-Based Nanoparticles for RNA Delivery
Various nanoparticle-based delivery systems have been developed for the encapsulation and protection of active cargoes. Lipid nanoparticles represent...
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Conformational Dynamic Studies of Prokaryotic Potassium Channels Explored by Homo-FRET Methodologies
FluorescenceFluorescence techniques have been widely used to shed light over the structure–function relationship of potassium channels for the last...
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SIRT1-modified human umbilical cord mesenchymal stem cells ameliorate experimental peritoneal fibrosis by inhibiting the TGF-β/Smad3 pathway
IntroductionPeritoneal fibrosis is a serious complication of long-term peritoneal dialysis (PD). Combination therapies are emerging as a promising...
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Ameliorative role of SIRT1 in peritoneal fibrosis: an in vivo and in vitro study
BackgroundPeritoneal fibrosis is one of the major complications induced by peritoneal dialysis (PD). Damaged integrity and function of peritoneum...
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Supported Molecular Matrix Electrophoresis
Distinct bands of mucins cannot be banded using a gel electrophoresis based on a molecular sieving effect due to their very large molecular weight...
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Measuring Mitotic Spindle and Microtubule Dynamics in Marine Embryos and Non-model Organisms
During eukaryotic cell divisionCell division a microtubule-based structure, the mitotic spindle, aligns and segregates chromosomes between daughter...
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Application of Archaea in Deubiquitinase-Like Enzyme Discovery and Activity Assay
Archaea can be used as microbial platforms to discover new types of deubiquitinase-like (DUB-like) enzymes and to produce ubiquitin/ubiquitin-like...
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Polyvinylamine and Its Derivative as Effective Carrier for Targeted Delivery of Small RNAs
Polymeric delivery systems could enable the fast- and low-side-effect transport of various RNA classes. Previously, we demonstrated that...
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Analysis of Phage Regulatory RNAs: Sequencing Library Construction from the Fraction of Small Prokaryotic RNAs Less Than 50 Nucleotides in Length
So far, bacterial regulatory sRNAs of length less than 50 nucleotides have been poorly understood, and a low number of such molecules has been...
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Characterization of Deubiquitinase Catalytic State Using a Structure-Based Approach
The activity of deubiquitinases (DUBs) is tightly regulated in eukaryotes via various mechanisms. One of the regulatory strategies is...
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Atomic Force Microscopy in the Characterization of the Structure of Cell Wall Components
Atomic force microscopy (AFM) has broken boundaries in the characterization of the supramolecular architecture of cell wall assemblies and single...
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Lyophilization and homogenization of biological samples improves reproducibility and reduces standard deviation in molecular biology techniques
Lyophilization is a cost-effective method for biological specimen preservation but detailed tissue-specific reference protocols are still lacking....
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Characterization of Prolyl-4-Hydroxylase Substrate Specificity Using Pichia pastoris as an Efficient Eukaryotic Expression System
The use of eukaryotic expression systems facilitates the heterologous expression of complex eukaryotic proteins in their post-translationally...
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Human Minor Salivary Glands: A Readily Available Source of Salivary Stem/Progenitor Cells for Regenerative Applications
Resident stem/progenitor cells within the secretory salivary glands offer a potential therapeutic resource for use in the regeneration of salivary...
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High Throughput Glycerate Kinase Activity Assay Using Crude Leaf Extract and Recombinant Enzyme to Determine Kinetic Parameters Km and Vmax Using a Microplate Reader
We describe an assay for measuring the activity of D-glycerate 3-kinase (GLYK) in a 96-well microplate format with the use of a set of coupling...
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Bone Histomorphometry and 18F-Sodium Fluoride Positron Emission Tomography Imaging: Comparison Between only Bone Turnover-based and Unified TMV-based Classification of Renal Osteodystrophy
Bone biopsy is the gold standard for characterization of renal osteodystrophy (ROD). However, the classification of the subtypes of ROD based on...
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Assessing the Bioavailability and Testing the Effectiveness of Nutraceuticals and Nanonutraceuticals
In recent days, foods rich in nutraceuticals are in demand because of their nature to prevent or heal several chronic diseases. Efficiency of...
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Nucleosome Core Particle Reconstitution with Recombinant Histones and Widom 601 DNA
Reconstitution of nucleosomes from recombinant histones and DNA is a widely used tool for studying nucleosome structure, dynamics, and interactions....
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Mechanical Actuation of Organoids in Synthetic Microenvironments
Organoids are a powerful model system to explore the role of mechanical forces in sculpting emergent tissue cytoarchitecture. The modulation of the...