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Visualizing the Replisome, Chromosome Breaks, and Replication Restart in Bacillus subtilis
Research over the last two decades has revealed that bacterial genomes are highly organized and that bacteria have sophisticated mechanisms in place...
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How asymmetric DNA replication achieves symmetrical fidelity
Accurate DNA replication of an undamaged template depends on polymerase selectivity for matched nucleotides, exonucleolytic proofreading of...
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Locus-Specific Analysis of Replication Dynamics and Detection of DNA–RNA Hybrids by Immuno Electron Microscopy
DNA–RNA hybrids can interfere with DNA replication, but the underlying intermediates and molecular mechanisms have remained elusive. Here, we...
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Replication and transcription machinery for ranaviruses: components, correlation, and functional architecture
BackgroundRanaviruses (family Iridoviridae ) are promiscuous pathogens that can infect across species barriers in poikilotherms and can replicate in...
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Mgs1 function at G-quadruplex structures during DNA replication
The coordinated action of DNA polymerases and DNA helicases is essential at genomic sites that are hard to replicate. Among these are sites that...
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BMI1 nuclear location is critical for RAD51-dependent response to replication stress and drives chemoresistance in breast cancer stem cells
Replication stress (RS) has a pivotal role in tumor initiation, progression, or therapeutic resistance. In this study, we depicted the mechanism of...
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Control of replication stress and mitosis in colorectal cancer stem cells through the interplay of PARP1, MRE11 and RAD51
Cancer stem cells (CSCs) are tumor subpopulations driving disease development, progression, relapse and therapy resistance, and their targeting...
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The structure-specific endonuclease complex SLX4–XPF regulates Tus–Ter-induced homologous recombination
Vertebrate replication forks arrested at interstrand DNA cross-links (ICLs) engage the Fanconi anemia pathway to incise arrested forks, ‘unhooking’...
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FANCD2 counteracts O6-methylguanine-induced mismatch repair-dependent apoptosis
BackgroundSn1-type alkylating agents methylate the oxygen atom on guanine bases thereby producing O 6 -methylguanine. This modified base could pair...
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TAS1553, a small molecule subunit interaction inhibitor of ribonucleotide reductase, exhibits antitumor activity by causing DNA replication stress
Ribonucleotide reductase (RNR) is composed of two non-identical subunits, R1 and R2, and plays a crucial role in balancing the cellular dNTP pool,...
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TRF2 recruits nucleolar protein TCOF1 to coordinate telomere transcription and replication
Telomeres are transcribed into telomeric RNA termed as TERRA. However, the transcription itself and excessive TERRA may interfere with telomere...
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Sonic hedgehog accelerates DNA replication to cause replication stress promoting cancer initiation in medulloblastoma
The mechanisms generating cancer-initiating mutations are not well understood. Sonic hedgehog (SHH) pathway activation is frequent in medulloblastoma...
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Asymmetric histone inheritance via strand-specific incorporation and biased replication fork movement
Many stem cells undergo asymmetric division to produce a self-renewing stem cell and a differentiating daughter cell. Here we show that, similarly to...
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Natural products from Streptomyces spp. as potential inhibitors of the major factors (holoRdRp and nsp13) for SARS-CoV-2 replication: an in silico approach
The COVID-19 pandemic caused unprecedented damage to humanity, and while vaccines have been developed, they are not fully effective against the...
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The PPR domain of mitochondrial RNA polymerase is an exoribonuclease required for mtDNA replication in Drosophila melanogaster
Mitochondrial DNA (mtDNA) replication and transcription are of paramount importance to cellular energy metabolism. Mitochondrial RNA polymerase is...
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Acute hydroxyurea-induced replication blockade results in replisome components disengagement from nascent DNA without causing fork collapse
During S phase, replication forks can encounter several obstacles that lead to fork stalling, which if persistent might result in fork collapse. To...
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Transcription shapes DNA replication initiation to preserve genome integrity
BackgroundEarly DNA replication occurs within actively transcribed chromatin compartments in mammalian cells, raising the immediate question of how...
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Rad53 limits CMG helicase uncoupling from DNA synthesis at replication forks
The coordination of DNA unwinding and synthesis at replication forks promotes efficient and faithful replication of chromosomal DNA. Disruption of...
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In Vivo Binding of Single-Stranded DNA-Binding Protein to Stalled Replication Fork Helicases
Understanding protein-protein interactions is key to unraveling protein function in vivo. Here we describe a dual/triple-plasmid system that enables...
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Role of HP1β during spermatogenesis and DNA replication
Heterochromatin protein 1β (HP1β), encoded by the Cbx1 gene, has been functionally linked to chromatin condensation, transcriptional regulation, and...
