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Visualizing the Replisome, Chromosome Breaks, and Replication Restart in Bacillus subtilis

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Chromosome Architecture

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2476))

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Abstract

Research over the last two decades has revealed that bacterial genomes are highly organized and that bacteria have sophisticated mechanisms in place to ensure their correct replication and segregation into progeny cells. Here we discuss techniques that can be used with live bacterial cells to analyze DNA replisome dynamics, double-strand chromosome breaks, and restart of repaired replication forks.

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Author information

Authors and Affiliations

  1. Centre for Bacterial Cell Biology, Biosciences Institute, Newcastle University, Newcastle upon Tyne, UK

    Hannah Gaimster, Charles Winterhalter, Alan Koh & Heath Murray

Authors
  1. Hannah Gaimster
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  2. Charles Winterhalter
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  3. Alan Koh
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  4. Heath Murray
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Corresponding author

Correspondence to Heath Murray .

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Editors and Affiliations

  1. Departments of Physics and Biology, University of York, York, UK

    Mark C. Leake

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Gaimster, H., Winterhalter, C., Koh, A., Murray, H. (2022). Visualizing the Replisome, Chromosome Breaks, and Replication Restart in Bacillus subtilis. In: Leake, M.C. (eds) Chromosome Architecture. Methods in Molecular Biology, vol 2476. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2221-6_18

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  • DOI: https://doi.org/10.1007/978-1-0716-2221-6_18

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2220-9

  • Online ISBN: 978-1-0716-2221-6

  • eBook Packages: Springer Protocols

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