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Carrot cells expressing the VP1 and VP2 poliovirus proteins effectively elicited mucosal immunity
The WHO has recommended a combined schedule of vaccination including the oral (OPV) and the inactivated injectable vaccine. Each of these vaccines...
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Pathogenic Escherichia coli: Virulence Factors and Their Antimicrobial Resistance
Escherichia coli (E. coli) falls under the family Enterobacteriaceae with the genus Escherichia. They are facultative anaerobic bacteria mostly at...
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Oral vaccination for sustainable disease prevention in aquaculture—an encapsulation approach
The prevalence of infectious diseases in the aquaculture industry and a limited number of safe and effective oral vaccines has imposed a challenge...
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Green biotherapeutics: overcoming challenges in plant-based expression platforms
Plant-based expression platforms offer a promising avenue to produce biotherapeutics due to their scalability, cost-effectiveness, and potential for...
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Hairy root cultures as a multitask platform for green biotechnology
Hairy root cultures serve as a useful tool in the experiments aiming to understand plant biology (e.g. functional analysis of gene function) which...
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In silico analyses of the genomes of three new bacteriocin-producing bacteria isolated from animal’s faeces
Here, we have analysed and explored the genome sequences of three newly isolated bacteria that were recently characterised for their probiotic...
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Molecular basis of a bacterial-amphibian symbiosis revealed by comparative genomics, modeling, and functional testing
The molecular bases for the symbiosis of the amphibian skin microbiome with its host are poorly understood. Here, we used the odor-producer Pseudomonas ...
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Rapid Detection of Diarrheagenic Escherichia coli by a New Multiplex Real-Time Quantitative PCR Assay
AbstractDiarrheagenic Escherichia coli (DEC) is a group of important foodborne pathogens that can spread and cause infection in humans. As a...
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Experimental Evaluation of In Silico Selected Signal Peptides for Secretory Expression of Erwinia Asparaginase in Escherichia coli
Erwinia chrysanthemi asparaginase is an important drug used in cancer treatment, especially in acute lymphoblastic leukemia (ALL). Escherichia coli ...
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Protein biomarker elucidation for the verification of biological agents in the taxonomic group of Gammaproteobacteria using tandem mass spectrometry
Some pathogenic microbes can be used for nefarious applications and instigate population-based fear. In a bio-threat scenario, rapid and accurate...
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Isolation and Analysis of Lipid Rafts from Neural Cells and Tissues
Lipid rafts are membrane areas characterized by the clustering of selected membrane lipids, as the result of their phase separation forming a...
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Bacterial bug-out bags: outer membrane vesicles and their proteins and functions
Among the major bacterial secretions, outer membrane vesicles (OMVs) are significant and highly functional. The proteins and other biomolecules...
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Resistance to ETEC F4/F18–mediated piglet diarrhoea: opening the gene black box
Diarrhoea, a significant problem in pig rearing industry affecting pre- and post-weaning piglets is caused by enterotoxigenic Escherichia coli ...
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A multi-epitope plant-made chimeric protein (LTBentero) targeting common enteric pathogens is immunogenic in mice
Key messageA plant-based multiepitopic protein (LTBentero) containing epitopes from ETEC, S. typhimurium , and V. parahaemolyticus was produced in...
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Comparative whole-genome and proteomics analyses of the next seed bank and the original master seed bank of MucoRice-CTB 51A line, a rice-based oral cholera vaccine
BackgroundWe have previously developed a rice-based oral vaccine against cholera diarrhea, MucoRice-CTB. Using Agrobacterium -mediated...
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Yeast Surface Display of Escherichia coli Enterotoxin and Its Effects of Intestinal Microflora and Mucosal Immunity
Enterotoxigenic Escherichia coli (ETEC) is a significant cause of infectious diarrhea in animals. In this study, yeast surface display technology was...
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Delivery of Virulence Factors by Bacterial Membrane Vesicles to Mammalian Host Cells
Bacterial membrane vesicles represent a universal secretion mechanism enabling both Gram-negative and Gram-positive organisms to transfer cargo to...
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Serine protease autotransporters of Enterobacteriaceae (SPATEs) are largely distributed among Escherichia coli isolated from the bloodstream
Extraintestinal pathogenic Escherichia coli (ExPEC) is the major cause of Gram-negative-related sepsis. Bacterial survival in the bloodstream is...
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Tannic acid prevents post-weaning diarrhea by improving intestinal barrier integrity and function in weaned piglets
BackgroundTannic acid (TA) is potential to reduce diarrhea in weaning pigs, but knowledge about the influence of TA on intestinal barrier integrity...
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Using the IPTG-Inducible Pgrac212 Promoter for Overexpression of Human Rhinovirus 3C Protease Fusions in the Cytoplasm of Bacillus subtilis Cells
Expression and secretion of recombinant proteins in the endotoxin-free bacterium, Bacillus subtilis , has been thoroughly studied, but overexpression...
