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The Efficiency of Gene Activation Using CRISPR/dCas9-Based Transactivation Systems Depends on the System Run Time
AbstractTransactivation systems are a promising application based on the CRISPR/Cas9 system and allow targeted control of gene expression levels in...
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An Induced Pluripotent Stem Cell Line (ICGi023-A) Obtained from a Patient with Parkinson’s Disease Associated Polymorphisms in LRRK2 and PINK1 Genes
AbstractParkinson’s disease is a neurodegenerative disease, and genetic variants are known in only 5% of cases. Analysis of clinical exome of a...
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Tumor suppressor let-7 acts as a key regulator for pluripotency gene expression in Muse cells
In embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), the expression of an RNA-binding pluripotency-relevant protein, LIN28, and...
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TNF-α suppresses sweat gland differentiation of MSCs by reducing FTO-mediated m6A-demethylation of Nanog mRNA
An effect of inhibition of tumor necrosis factor-α (TNF-α) on differentiation of mesenchymal stromal cells (MSCs) has been demonstrated, but the...
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A 3D “sandwich” co-culture system with vascular niche supports mouse embryo development from E3.5 to E7.5 in vitro
BackgroundVarious methods for ex utero culture systems have been explored. However, limitations remain regarding the in vitro culture platforms used...
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Hypoxia-induced immortalization of primary cells depends on Tfcp2L1 expression
Cellular senescence is a stress response mechanism that induces proliferative arrest. Hypoxia can bypass senescence and extend the lifespan of...
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G-quadruplex structural dynamics at MAPK12 promoter dictates transcriptional switch to determine stemness in breast cancer
P38γ (MAPK12) is predominantly expressed in triple negative breast cancer cells (TNBC) and induces stem cell (CSC) expansion resulting in decreased...
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Generation of an Induced Pluripotent Stem Cell Line, ICGi042-A, by Reprogramming Peripheral Blood Mononuclear Cells from a Parkinson’s Disease Patient with c.1000G>A Mutation in the LRRK2 Gene
AbstractThe search for new polymorphisms associated with hereditary diseases is important for diagnostics and the study of the disease’s development...
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Estrogen-Related Receptor Alpha (ERRα) Promotes Cancer Stem Cell-Like Characteristics in Breast Cancer
Cancer stem cells drive tumor initiation, invasion, metastasis and recurrence. In the present study, we have evaluated the role of ERRα in the...
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p53 isoform expression promotes a stemness phenotype and inhibits doxorubicin sensitivity in breast cancer
In breast cancer, dysregulated TP53 expression signatures are a better predictor of chemotherapy response and survival outcomes than TP53 mutations....
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Generation of Induced Pluripotent Stem Cell Lines ICGi022-A-3, ICGi022-A-4, and ICGi022-A-5 with p.Asn515del Mutation Introduced in MYBPC3 Using CRISPR/Cas9
AbstractUp to 60% of hypertrophic cardiomyopathy cases are due to mutations responsible for sarcomere functioning. However, clinical significance is...
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Chemokine CCL2 Activates Hypoxia Response Factors Regulating Pluripotency and Directed Endothelial Differentiation of Human Pluripotent Stem Cells
AbstractHuman pluripotent stem cell (PSC) research is currently focused on selecting conditions and growth factors that better mimic preimplantation...
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Endothelial β-catenin upregulation and Y142 phosphorylation drive diabetic angiogenesis via upregulating KDR/HDAC9
BackgroundDiabetic angiogenesis is closely associated with disabilities and death caused by diabetic microvascular complications. Advanced glycation...
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In Vitro Generated Equine Hepatic-Like Progenitor Cells as a Novel Potent Cell Pool for Equine Metabolic Syndrome (EMS) Treatment
Equine metabolic syndrome (EMS) is recognized as one of the leading cause of health threatening in veterinary medicine worldwide. Recently, PTP1B...
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Lineage regulators TFAP2C and NR5A2 function as bipotency activators in totipotent embryos
During the first lineage segregation, a mammalian totipotent embryo differentiates into the inner cell mass (ICM) and trophectoderm (TE). However,...
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Integrative Analysis of Ovarian Serious Adenocarcinoma to Understand Disease Network Biology
Ovarian cancer (OC) is the third leading gynecological malignancy in females that is silent and leads to significant deaths annually. As per GLOBOCAN...
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Generation of an Induced Pluripotent Stem Cell Line, ICGi043-A, by Reprogramming Peripheral Blood Mononuclear Cells from a Parkinson’s Disease Patient with p.G2019S Mutation in the LRRK2 Gene
AbstractThe pathological variant p.G2019S in the LRRK2 gene leads to the occurrence of a hereditary form of Parkinson’s disease (PD) and affects 7%...
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Transgenic Lines of Human Induced Pluripotent Stem Cells ICGi022-A-6 and ICGi022-A-7 with Doxycycline-Inducible Variants of Programmable Nuclease AsCas12a
AbstractGenome editing in human pluripotent stem cells using programmable nucleases makes it possible to create models of hereditary pathologies...
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Molecular Insights into Endometrial Cancer in Mice
Pluripotent, very small embryonic-like stem cells (VSELs) and the ‘progenitors’ endometrial stem cells (EnSCs) along with associated molecular...
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Zyxin regulates embryonic stem cell fate by modulating mechanical and biochemical signaling interface
Biochemical signaling and mechano-transduction are both critical in regulating stem cell fate. How crosstalk between mechanical and biochemical cues...
