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Genetic and Phenotypic Analysis of Ovule Development in Arabidopsis
The plant seed is a remarkable structure that represents the single most important energy source in global diets. The stages of reproductive growth...
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Laser capture microdissection for transcriptomic profiles in human skin biopsies
BackgroundThe acquisition of reliable tissue-specific RNA sequencing data from human skin biopsy represents a major advance in research. However, the...
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Single Cell Analysis of Mitochondrial DNA Deletions
Mitochondrial DNA (mtDNA) deletions underpin mitochondrial dysfunction in human tissues in aging and disease. The multicopy nature of the...
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Long non-coding RNA ZFAS1 is a major regulator of epithelial-mesenchymal transition through miR-200/ZEB1/E-cadherin, vimentin signaling in colon adenocarcinoma
Colon adenocarcinoma is a common cause of cancer-related deaths worldwide. Epithelial-mesenchymal transition is a major regulator of cancer...
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Tissue Glycome Map**: Lectin Microarray-Based Differential Glycomic Analysis of Formalin-Fixed Paraffin-Embedded Tissue Sections
Lectin microarray (LMA) is a high-sensitive glycan analysis technology used to obtain global glycomic profiles of both N- and O-glycans attached not...
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Deep Visual Proteomics defines single-cell identity and heterogeneity
Despite the availabilty of imaging-based and mass-spectrometry-based methods for spatial proteomics, a key challenge remains connecting images with...
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Reconstructing phylogenetic trees from genome-wide somatic mutations in clonal samples
Phylogenetic trees are a powerful means to display the evolutionary history of species, pathogens and, more recently, individual cells of the human...
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Comparative analysis of differentially abundant proteins quantified by LC–MS/MS between flash frozen and laser microdissected OCT-embedded breast tumor samples
BackgroundProteomic studies are typically conducted using flash-frozen (FF) samples utilizing tandem mass spectrometry (MS). However, FF specimens...
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Protoplast Isolation for Plant Single-Cell RNA-seq
The growth and development of plants depends on diversified gene expression in different cell types. Compared to traditional bulk RNA sequencing,...
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Application of Label-Free Proteomics for Quantitative Analysis of Urothelial Carcinoma and Cystitis Tissue
A label-free approach Label-free approaches based on a highly reproducible and stable workflow allows for quantitative...
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LCM and RNA-seq analyses revealed roles of cell cycle and translational regulation and homoeolog expression bias in cotton fiber cell initiation
BackgroundCotton fibers provide a powerful model for studying cell differentiation and elongation. Each cotton fiber is a singular and elongated cell...
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Laser Microdissection of Phytoplasma-Infected Grapevine Leaf Phloem Tissue for Gene Expression Study
Phytoplasmas have been found confined mainly in leaf phloem sieve elements. In spite of this, few researches have been focused on the infected phloem...
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Analysis of the transcriptome of bovine endometrial cells isolated by laser micro-dissection (1): specific signatures of stromal, glandular and luminal epithelial cells
BackgroundA number of studies have examined mRNA expression profiles of bovine endometrium at estrus and around the peri-implantation period of...
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Spatially resolved analysis of FFPE tissue proteomes by quantitative mass spectrometry
Bottom-up mass spectrometry–based proteomics relies on protein digestion and peptide purification. The application of such methods to broadly...
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RNA-Seq analysis reveals potential regulators of programmed cell death and leaf remodelling in lace plant (Aponogeton madagascariensis)
BackgroundThe lace plant ( Aponogeton madagascariensis ) is an aquatic monocot that develops leaves with uniquely formed perforations through the use...
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High-resolution spatiotemporal transcriptome analyses during cellularization of rice endosperm unveil the earliest gene regulation critical for aleurone and starchy endosperm cell fate specification
The major tissues of the cereal endosperm are the starchy endosperm (SE) in the inner and the aleurone layer (AL) at the outer periphery. The fates...
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Using Giant Scarlet Runner Bean (Phaseolus coccineus) Embryos to Dissect the Early Events in Plant Embryogenesis
The giant embryo of the scarlet runner bean (Phaseolus coccineus) has been used historically to investigate the molecular and developmental processes...
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Multi-omics profiling of living human pancreatic islet donors reveals heterogeneous beta cell trajectories towards type 2 diabetes
Most research on human pancreatic islets is conducted on samples obtained from normoglycaemic or diseased brain-dead donors and thus cannot...
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Transcriptome profiling of laser-captured germ cells and functional characterization of zbtb40 during 17alpha-methyltestosterone-induced spermatogenesis in orange-spotted grouper (Epinephelus coioides)
BackgroundSpermatogenesis is an intricate process regulated by a finely organized network. The orange-spotted grouper ( Epinephelus coioides ) is a...
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How to explore what is hidden? A review of techniques for vascular tissue expression profile analysis
The evolution of plants to efficiently transport water and assimilates over long distances is a major evolutionary success that facilitated their...
