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Learning Towards Maturation of Defined Feeder-free Pluripotency Culture Systems: Lessons from Conventional Feeder-based Systems
Pluripotent stem cells (PSCs) are widely recognized as one of the most promising types of stem cells for applications in regenerative medicine,...
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Harnessing nanoparticle-mediated elicitation in plant tissue culture: a promising approach for secondary metabolite production
In vitro, plant cell cultures have emerged as a promising and sustainable method for producing valuable and economically important metabolites in a...
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Hydroxyapatite nanoparticles: an alternative to conventional phosphorus fertilizers in acidic culture media
BackgroundTraditional phosphorus fertilizers generally have low efficiencies due to their immobilization in soil, and a large part of these...
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Three-Dimensional Cell Culture System for Tendon Tissue Engineering
Tendon, connective tissue between bone and muscle has unique component of the musculoskeletal system. It plays important role for transporting...
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Direct Cardiac Reprogramming: Current Status and Future Prospects
Advances in cellular reprogramming articulated the path for direct cardiac lineage conversion, bypassing the pluripotent state. Direct cardiac...
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A practical workflow for forensic species identification using direct sequencing of real-time PCR products
BackgroundForensic scientists are often required to identify species of unknown biological samples. Although methods based on sequencing of DNA...
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Investigation of Deficiency and Excess Nutrients Under in Vitro Culture Conditions in Petunia hybrida
Plants are one of the most important factors in human life and need special elements for growth and development. The amount of the necessary elements...
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Effects of process intensification on homogeneity of an IgG1:κ monoclonal antibody during perfusion culture
AbstractThe pharmaceutical industry employs various strategies to improve cell productivity. These strategies include process intensification,...
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Efficient modification and preparation of circular DNA for expression in cell culture
DNA plasmids are an essential tool for delivery and expression of RNAs and proteins in cell culture experiments. The preparation of plasmids...
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Establishment of axenic culture from basidiospores of an ectomycorrhizal fungus Astraeus asiaticus and A. odoratus
Astraeus is a genus of edible ectomycorrhizal star-shaped fungus with commercial value. The inner tissue of basidiomata has previously been utilized...
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Development of three-dimensional (3D) spheroid culture system from rainbow trout kidney cell line (RTK) for in vitro production of fish viral pathogen
The present study aimed to generate 3D spheroid cultures from three rainbow trout (RTE, RTK and RTS) cell lines by simple and reproducible methods...
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Haploid induction through ovary culture in cucumber
Haploidization technique is the modern and advanced breeding technique used to enhance economically essential crops, including cucumber, to meet...
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Laser-Structured Si and PLGA Inhibit the Neuro2a Differentiation in Mono- and Co-Culture with Glia
Background:The first step towards a successful neural tissue engineering therapy is the development of an appropriate scaffold and the in vitro ...
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New insights into the influence of pre-culture on robust solvent production of C. acetobutylicum
AbstractClostridia are known for their solvent production, especially the production of butanol. Concerning the projected depletion of fossil fuels,...
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Use of GC-IMS for detection of volatile organic compounds to identify mixed bacterial culture medium
In order to explore the possibility to identify common wound infection bacteria in mixed culture with gas chromatograph-ion migration spectroscopy...
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Customized small-sized clinostat using 3D printing and gas-permeable polydimethylsiloxane culture dish
Over the past few decades, research on life in space has increased. Owing to the expensive nature of and the challenges associated with conducting...
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Reversible suspension culture of human vascular smooth muscle cells using the functional biopolymer FP003
Human vascular smooth muscle cells (SMCs) are adherent cells, and they cannot survive without scaffolds in suspension culture. Here, we aimed to...
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Use of flow cytometry method to detect contaminations of platelet suspensions
In this study, it was aimed to investigate bacterial contamination in apheresis platelet suspensions (APS) by automated blood culture system and flow...
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Visual Analysis of Research Status and Trends of Dendrobium Tissue Culture Based on CiteSpace
AbstractDendrobium species, esteemed as a group of orchid plants with important ornamental and medicinal value, are currently experiencing...
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Induction, growth, and characteristics of embryonic cell suspension culture of wild bananas (Musa acuminata ssp.)
Wild bananas and their relatives are potentially utilized for pre-breeding due to their genetic diversity, disease resistance, and tolerance to...
