Abstract
Astraeus is a genus of edible ectomycorrhizal star-shaped fungus with commercial value. The inner tissue of basidiomata has previously been utilized to study the macro– and micro-cultural traits of Astraeus; though, a handful of studies have focused on basidiospores. Here we report the first pure cultural traits of Indian Astraeus asiaticus and A. odoratus obtained from basidiospores drop**/dusting method. Four distinct solid culture media namely potato dextrose agar (PDA), modified Melin–Norkrans (MMN), modified Norklan’ s C (MNC), and Yeast media (YM) were used to culture the basidiospores. After about 42 days of culture in dark at 30 °C all the cultures exhibit diffused pale–yellow pigmentation followed by radial mycelial growth with fluffy and velvety surface. Monomitic, generative hyphae, and septate clamps with paariage branching were observed which are the typical traits of the cultured mycelium. A. odoratus were successfully grown on all the four media but attempts to grow A. asiaticus on MMN, MNC, and YM were unsuccessful. The fungal culture’s identity was validated by phylogenetical approach using ITS and LSU nrDNA. The findings suggest PDA medium as the most suitable medium for culture of Astraeus and provide critical information on the culture traits that could be useful for wild mushroom cultivation thus increasing the country’s mycoprotein richness.
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Acknowledgements
We would like to express our gratitude to the Head of the Department of Botany, DSPMU, Ranchi, Jharkhand, the Principal of Bangabasi Evening College, Kolkata, West Bengal, India and Head of Department of Microbiology, Faculty of Science, Srinakharinwirot University, Bangkok, and Dean of Faculty of Science, Nakhon Phanom University, Nakhon Phanom, Thailand, for providing the necessary equipment. We are thankful to the Scientist of Central National Herbarium (CAL) and Dr. Kanad Das, of the Botanical Survey of India, Howrah, West Bengal, India, for their motivation and guidance.
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The ideas and project were developed by SL, NS, CP, and VV. VV, SST, PTO and PTA, were involved in the research and investigation process, particularly in experimentation. VV, SL, NS, and CP have equally contributed to data interpretation, analysis and writing of the manuscript. MEH and VV were doing line diagrams. All of the authors provided constructive feedback on how to implement the study, analysis, and development of the article. The version to be submitted, as well as any revised versions, received final approval from SL, NS, and CP.
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Vishal, V., Tigga, S.S., Thongsuwan, P. et al. Establishment of axenic culture from basidiospores of an ectomycorrhizal fungus Astraeus asiaticus and A. odoratus. Indian Phytopathology (2024). https://doi.org/10.1007/s42360-024-00768-4
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DOI: https://doi.org/10.1007/s42360-024-00768-4