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“Heart Cells” Derived from Pluripotent Stem Cells and Therapeutic Applications
The field of cardiovascular regenerative medicine continues to evolve with the hope of discovering effective and durable treatments for patients...
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Mammary Stem Cells Methods and Protocols
This second edition provides an overview of recent developments and approaches used by researchers to investigate the properties and functions of...
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Differentiation of Hepatic Stellate Cells from Pluripotent Stem Cells
Hepatic stellate cells (HSCs) are non-parenchymal cells with a mesenchymal origin involved in vitamin A storage and extracellular matrix (ECM)...
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Optimizing Solar Photovoltaic Cells
Replacement of fossil fuel-based energy sources in agriculture becomes necessary because of its restricted supply and impact on the environment. In...
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Bioencapsulation of Oocytes and Granulosa Cells
A protocol for the encapsulation in sodium alginate of granulosa cells in primary culture and coculture of oocyte-cumulus complexes is reported....
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The Hallmarks of Circulating Hybrid Cells
While tumor metastases represent the primary driver of cancer-related mortality, our understanding of the mechanisms that underlie metastatic...
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Restoring β Cells Population Through In Situ Differentiation of Residential Pancreatic Cells
Diabetes mellitus (DM) is a global health problem leading to morbidity, mortality, and compromising the quality of life. DM is well characterized by...
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Induction of Human Extraembryonic Mesoderm Cells from Naive Pluripotent Stem Cells
The human extraembryonic mesoderm (EXM) is an important tissue in the postimplantation embryo which is specified before gastrulation in primates but...
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Renal Organoids from Whole Kidney Cells
Organoid technology, as a three-dimensional (3D) culture method, provides a feasible tool to self-organize multiple types of organ-specific cells,...
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Isolation of Human Adipose-Derived Stem Cells
Human adipose-derived stromal/stem cells (hASCs) are a promising source of adult stem cells used in numerous applications in regenerative medicine....
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Methods for Co-culture of Primary Human Extravillous Trophoblast Cells and Uterine Natural Killer Cells
During early pregnancy, fetal-derived extravillous trophoblast cells (EVT) from the placenta invade the maternal decidua and inner third of the...
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Differentiation of Human Fetal Muscle Stem Cells from Induced Pluripotent Stem Cells
Most muscular dystrophies are the result of genetic disorders. There is currently no effective treatment for these progressive diseases except...
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Monitoring the Interaction Between Dendritic Cells and T Cells In Vivo with LIPSTIC
Interactions between different cell types are key for immune function. Traditionally, interactions have been investigated in vivo by intravital...
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Artificial Cells for Dissecting Exocytosis
The fusion of vesicles and exocytosis release of neurotransmitters into the extracellular space for detection and chemical signal decoding by...
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Isolation and In Vitro Culture of Germ Cells and Sertoli Cells from Human Fetal Testis
In the human fetal testis, fetal germ cells (FGCs) are progressively surrounded by supporting Sertoli cells inside seminiferous cords. During the...
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Targeting senescent cells with NKG2D-CAR T cells
This study investigates the efficacy of NKG2D chimeric antigen receptor (CAR) engineered T cells in targeting and eliminating stress-induced...
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Mesenchymal Stem Cells, Cancer Stem Cells (CSCs), and Circulating Tumor Cells (CTCs): Roles and Combinations in Tumor Growth and Dissemination
Mesenchymal stem cells are considered the key cells in tissue regeneration after damage. MSCs play a fundamental role in cancer since they can be...
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1 Phospholipid synthesis in mammalian cells
Phospholipids are the main components of biological membranes and as such act as the major permeability barrier between cells and the extracellular...
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Differentiated Epithelial Cells of the Gut
The intestine is a prime example of self-renewal where stem cells give rise to progenitor cells called transit-amplifying cells which differentiate...
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Cryo-Focused Ion Beam Milling of Cells
Cryo-electron tomography (cryo-ET) is a robust technique for bringing structural biology into the cellular context. However, transmission electron...