Abstract
A protocol for the encapsulation in sodium alginate of granulosa cells in primary culture and coculture of oocyte-cumulus complexes is reported. Sodium alginate forms strong gels when jellified with barium ions, allowing the self-organization of cells into a 3D structure. This method of encapsulation is simple and cheap, allowing the culture of cells in a three-dimensional fashion.
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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
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Faustini, M., Agradi, S., Vigo, D., Torre, M.L., Curone, G. (2024). Bioencapsulation of Oocytes and Granulosa Cells. In: Baratta, M. (eds) Epithelial Cell Culture. Methods in Molecular Biology, vol 2749. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3609-1_10
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DOI: https://doi.org/10.1007/978-1-0716-3609-1_10
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-3608-4
Online ISBN: 978-1-0716-3609-1
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