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Fluorescent In Situ Detection of RNA–Protein Interactions in Intact Cells by RNA-PLA
RNA–protein proximity ligation assay (RNA-PLA) enables the detection of specific RNA–protein interactions in fixed cells. In RNA-PLA, bridging and...
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Iron Accumulation of Halophytes in the Littoral Zone of the White Sea
AbstractThe accumulations of iron in the halophytic species Triglochin maritima L., Plantago maritima L., Aster tripolium L., and Zostera marina L....
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Toxic metal pollution of aquatic ecosystems of European Union nature protection areas in a region of intensive agriculture (Lake Gopło, Poland)
The paper presents the results of research into toxic metal concentrations in the surface layer of bottom sediments in Lake Gopło. The research...
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Reprogramming Human Female Adipose Mesenchymal Stem Cells into Primordial Germ Cell-Like Cells
In the last two decades, considerable progress has been made in the derivation of mammalian germ cells from pluripotent stem cells such as Embryonic...
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Unveiling the mechanistic link between extracellular amyloid fibrils, mechano-signaling and YAP activation in cancer
The tumor microenvironment is a complex ecosystem that plays a critical role in cancer progression and treatment response. Recently, extracellular...
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Loss of ORP3 induces aneuploidy and promotes bladder cancer cell invasion through deregulated microtubule and actin dynamics
We have recently shown that loss of ORP3 leads to aneuploidy induction and promotes tumor formation. However, the specific mechanisms by which ORP3...
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Visualizing Neurons Under Tension In Vivo with Optogenetic Molecular Force Sensors
The visualization of mechanical stress distribution in specific molecular networks within a living and physiologically active cell or animal remains...
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Elucidating Immune Monitoring of Tissue-Resident Macrophages by Intravital Microscopy
Intravital microscopy is an invaluable tool to study in real time the dynamic behavior of leukocytes in vivo. We describe herein a simple protocol...
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Expression study of five genes involved in floral organ development in multiple seeded rice
Rice cultivar Jugal is a unique floral organ mutant from South Bengal and Odisa, the two sister states of Eastern India, carries more than one...
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Combined RNA interference and gene replacement therapy targeting MFN2 as proof of principle for the treatment of Charcot–Marie–Tooth type 2A
Mitofusin-2 (MFN2) is an outer mitochondrial membrane protein essential for mitochondrial networking in most cells. Autosomal dominant mutations in...
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Hypoxia is fine-tuned by Hif-1α and regulates mesendoderm differentiation through the Wnt/β-Catenin pathway
BackgroundHypoxia naturally happens in embryogenesis and thus serves as an important environmental factor affecting embryo development. Hif-1α, an...
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Diversity of fungal endophytes from mangrove plants of Santa Catarina Island, Brazil
The mangrove ecosystem plays a crucial role in preserving the biodiversity of plants, animals, and microorganisms that are essential for materials...
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The nucleoporin NUP160 and NUP96 regulate nucleocytoplasmic export of mRNAs and participate in ethylene signaling and response in Arabidopsis
Key messageArabidopsis nucleoporin involved in the regulation of ethylene signaling via controlling of nucleocytoplasmic transport of mRNAs. ...
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The Most Endemic Taxon of an Area of Endemism: Harvestmen (Opiliones) Fauna of NAF and Its Contribution to Biogeography and Conservation
Harvestmen are mostly composed of very endemic and narrow-ranged species, especially those living in tropical forests. Hygrophily, cryptic behavior,...
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Quantitative Spatio-temporal Analysis of Phagosome Maturation in Live Cells
Phagocytosis and phagosome maturation are central processes to the development of the innate and adaptive immune response. Phagosome maturation is a...
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Interrupted hibernation of the gall-inducer affects its parasitoids – a case study on some gall communities of Diplolepis rosae (Hymenoptera: Cynipidae) in Bulgaria
Gall communities of the bedeguar gall wasp, Diplolepis rosae , have been explored by many authors during the years because of their species richness...
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Assessment of the probiotic potential of lactic acid bacteria isolated from kefir grains: evaluation of adhesion and antiproliferative properties in in vitro experimental systems
The main objective of this study was to isolate lactic acid bacteria from kefir grains and investigate their probiotic potential. In this study, 48...
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Single-Cell Imaging of mRNA by Target RNA-Initiated RCA
We present a powerful method for direct mRNA detection based on ligation-based recognition and in situ amplification, capable of single-cell imaging...
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Fish sounds of photic and mesophotic coral reefs: variation with depth and type of island
Mesophotic Coral Ecosystems remain largely unexplored. The aim of this study was to determine how the acoustic fish biodiversity varied depending on...
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Simple modeling of familial Alzheimer’s disease using human pluripotent stem cell-derived cerebral organoid technology
BackgroundCerebral organoids (COs) are the most advanced in vitro models that resemble the human brain. The use of COs as a model for Alzheimer’s...
