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1. Extracellular vesicles are the main contributor to the non-viral protected extracellular sequence space

   Environmental virus metagenomes, commonly referred to as “viromes”, are typically generated by physically separating virus-like particles (VLPs) from...

   Dominik Lücking, Coraline Mercier, ... Susanne Erdmann in ISME Communications

   Article Open access 17 October 2023
   

2. Highly Purified Top1-Bound DNA Fragments

   Topoisomerase 1–DNA cleavage complexes (Top1ccs) form at DNA sites of Top1 activity and are increased by a highly specific anticancer drug...

   Jessica Marinello, Giovanni Capranico in R-Loops

   Protocol 2022
   

3. CryoEM Investigation of Three-Dimentional Structure of the Stx-Converting Bacteriophage ϕ24B

   Abstract —

   A modified method for culturing, concentrating, and purifying phage ϕ24B preparations was developed. In particular, a new lysogenic...

   A. S. Kuznetsov, A. V. Moiseenko, ... A. V. Letarov in Microbiology

   Article 14 May 2024
   

4. The effect of the ¹³C abundance of soil microbial DNA on identifying labelled fractions after ultracentrifugation

   Abstract

   DNA-based stable isotope probing (DNA-SIP) technology has been widely employed to trace microbes assimilating target substrates. However, the...

   Juan Wang, Huaiying Yao, **an Zhang in Applied Microbiology and Biotechnology

   Article Open access 03 May 2024
   

5. Calcium-Activated Chloride Channels: The Role of Potassium Ions

   Abstract

   Using the patch–clamp method in the whole-cell configuration, it was shown that external potassium ions play an important role in the...

   V. L. Zamoyski, A. V. Gabrelian, V. V. Grigoriev in Biology Bulletin

   Article 21 May 2024
   

6. Dual function of a highly conserved bacteriophage tail completion protein essential for bacteriophage infectivity

   Infection of bacteria by phages is a complex multi-step process that includes specific recognition of the host cell, creation of a temporary breach...

   Isabelle Auzat, Malika Ouldali, ... Paulo Tavares in Communications Biology

   Article Open access 16 May 2024
   

7. Arabidopsis transcriptomic analysis reveals cesium inhibition of root growth involves abscisic acid signaling

   Main conclusion

   This is the first report on the involvement of abscisic acid signaling in regulating post-germination growth under Cs stress, not...

   Wen-Dee Ong, Yuko Makita, ... Ryoung Shin in Planta

   Article 15 January 2024
   

8. Mucin Purification and Printing Natural Mucin Microarrays

   Mucin glycosylation is the key facilitator of microbial attachment and nutrition and it varies according to biological location, health and disease...

   Marie Le Berre, Jared Q. Gerlach, ... Michelle Kilcoyne in Glycan Microarrays

   Protocol 2022
   

9. Enhanced Methodologies for Investigating the Electrophysiological Characteristics of Endogenous Pannexin 1 Intercellular Cell–Cell Channels

   Gap junctions, pivotal intercellular conduits, serve as communication channels between adjacent cells, playing a critical role in modulating membrane...

   Nicolás Palacios-Prado, Teresa Vergara, Juan C. Sáez in Connexin Hemichannels

   Protocol 2024
   

10. Tough Decoy-Mediated Cardiac Gene Suppression

    MicroRNA (miRNA) is a small, non-coding RNA molecule (~22 nucleotides) that acts as a post-transcriptional gene regulator, primarily by inhibiting...

    Changwon Kho in Cardiac Gene Therapy

    Protocol 2022
    

11. Trennverfahren und allgemeine Analysemethoden

    Zur Analyse biochemischer Vorgänge müssen oft Zellbestandteile voneinander getrennt werden. Die Biomoleküle können aufgrund ihrer Unterschiede in...

    Philipp Christen, Rolf Jaussi, Roger Benoit in Biochemie und Molekularbiologie

    Chapter 2024
    

12. Preclinical characterization and in silico safety assessment of three virulent bacteriophages targeting carbapenem-resistant uropathogenic Escherichia coli

    Phage therapy has recently been revitalized in the West with many successful applications against multi-drug-resistant bacterial infections. However,...

    Gunaraj Dhungana, Roshan Nepal, ... Rajani Malla in International Microbiology

    Article Open access 22 March 2024
    

13. Identification of diverse antibiotic resistant bacteria in agricultural soil with H₂¹⁸O stable isotope probing combined with high-throughput sequencing

    Background

    We aimed to identify bacteria able to grow in the presence of several antibiotics including the ultra-broad-spectrum antibiotic meropenem...

    Marcela Hernández, Shamik Roy, ... Marc G. Dumont in Environmental Microbiome

    Article Open access 18 April 2023
    

14. Deleterious effect of LiCl on honeybee (Aphis mellifera) grubs and no effect on Varroa mites (Varroa destructor) under normal beekee** management

    A 2-year field experiment was performed to test lithium chloride, LiCl, application in a normal beekee** management system. The effect of LiCl on...

    Imre Demeter, Miklós Sárospataki, ... Adalbert Balog in Biologia Futura

    Article Open access 06 December 2023
    

15. Unexpected impairment of I_Na underpins reentrant arrhythmias in a knock-in swine model of Timothy syndrome

    Timothy syndrome 1 (TS1) is a multi-organ form of long QT syndrome associated with life-threatening cardiac arrhythmias, the organ-level dynamics of...

    Andreu Porta-Sánchez, Andrea Mazzanti, ... Silvia G. Priori in Nature Cardiovascular Research

    Article Open access 11 December 2023
    

16. Conventional and Basic Genomic Techniques

    In this chapter, certain very basic and primary technologies pertaining to genomics have been recapitulated. The topics covered include basic...

    Aruna Pal in Protocols in Advanced Genomics and Allied Techniques

    Protocol 2022
    

17. The formation of K_V2.1 macro-clusters is required for sex-specific differences in L-type Ca_V1.2 clustering and function in arterial myocytes

    In arterial myocytes, the canonical function of voltage-gated Ca _V 1.2 and K _V 2.1 channels is to induce myocyte contraction and relaxation through their...

    Collin Matsumoto, Samantha C. O’Dwyer, ... L. Fernando Santana in Communications Biology

    Article Open access 14 November 2023
    

18. Characterization of a novel lytic bacteriophage VPMCC14 which efficiently controls Vibrio harveyi in Penaeus monodon culture

    Vibrio harveyi causes luminous vibriosis diseases in shrimp, which lead to shrimp mortalities. Considering the emergence of antibiotic-resistant...

    Priyanka Kar, Smita Ghosh, ... Kuntal Ghosh in International Microbiology

    Article 03 December 2023
    

19. Recording of Diurnal Gene Expression in Peripheral Organs of Mice Using the RT-Biolumicorder

    There is high interest in investigating the daily dynamics of gene expression in mammalian organs, for example, in liver. Such studies help to...

    Georgia Katsioudi, Alejandro Osorio-Forero, ... David Gatfield in Circadian Regulation

    Protocol 2022
    

20. The Effects of Phenanthrene on the Electrical Activity in the Heart of Shorthorn Sculpin (Myoxocephalus scorpio)

    Abstract

    Phenanthrene (Phen) is one of the widespread species of polyaromatic hydrocarbons, a tricyclic compound, component of crude oil and...

    T. S. Filatova, V. B. Mikhailova, ... D. V. Abramochkin in Journal of Evolutionary Biochemistry and Physiology

    Article 01 December 2022