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Circadian Regulation

Methods and Protocols

  • Book
  • © 2022

Overview

Editors:
  1. Guiomar Solanas

    1. Institute for Research in Biomedicine, Barcelona Institute for Science and Tech, Barcelona, Spain

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    You can also search for this editor in PubMed   Google Scholar

  2. Patrick -Simon Welz

    1. Hospital del Mar Medical Research Institute (IMIM), Barcelona, Spain

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2482)

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About this book

This volume details methods on several aspects of circadian research. Chapters guide readers through the latest techniques and a wide variety of daily rhythmic processes, model organisms, circadian rhythms in the SCN and in peripheral organs, and describing in vitro systems and in silico methods. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

 

Authoritative and cutting-edge, Circadian Regulation: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field. 

Chapters 3, 4, 8, and 17  are available open access under a Creative Commons Attribution 4.0 International License via springer.longhoe.net.

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Table of contents (27 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Sleep Under Preindustrial Conditions: What We Can Learn from It

    • Leandro Casiraghi, Horacio O. de la Iglesia
    Pages 1-14

  3. The Structure-Based Molecular-Docking Screen Against Core Clock Proteins to Identify Small Molecules to Modulate the Circadian Clock

    • Seref Gul, Ibrahim Halil Kavakli
    Pages 15-34

  4. Analysis of Complex Circadian Time Series Data Using Wavelets

    • Christoph Schmal, Gregor Mönke, Adrián E. Granada
    Pages 35-54Open Access

  5. Mathematical Modeling in Circadian Rhythmicity

    • Marta del Olmo, Saskia Grabe, Hanspeter Herzel
    Pages 55-80Open Access

  6. Bioinformatics and Systems Biology of Circadian Rhythms: BIO_CYCLE and CircadiOmics

    • Muntaha Samad, Forest Agostinelli, Pierre Baldi
    Pages 81-94

  7. Cell-Based Phenotypic Screens to Discover Circadian Clock-Modulating Compounds

    • Megumi Hatori, Tsuyoshi Hirota
    Pages 95-104

  8. Methods for Assessing Circadian Rhythms and Cell Cycle in Intestinal Enteroids

    • Miri Park, Yuhui Cao, Christian I. Hong
    Pages 105-124

  9. Using ALLIGATORs to Capture Circadian Bioluminescence

    • Aiwei Zeng, John S. O’Neill
    Pages 125-135Open Access

  10. Studying Circadian Clock Entrainment by Hormonal Signals

    • Violetta Pilorz, Iwona Olejniczak, Henrik Oster
    Pages 137-152

  11. In Vitro Assays for Measuring Intercellular Coupling Among Peripheral Circadian Oscillators

    • Anna-Marie Finger
    Pages 153-167

  12. Circadian Control of Transcriptional and Metabolic Rhythms in Primary Hepatocytes

    • Sung Kook Chun, Selma Masri
    Pages 169-179

  13. Electrophysiology of the Suprachiasmatic Nucleus: Single-Unit Recording

    • Martha U. Gillette, Jennifer W. Mitchell
    Pages 181-189

  14. Anatomical Methods to Study the Suprachiasmatic Nucleus

    • Eric L. Bittman
    Pages 191-210

  15. Circadian Analysis of Rodent Locomotor Activity in Home Cages

    • Paul Petrus, Paolo Sassone-Corsi
    Pages 211-215

  16. Recording of Diurnal Gene Expression in Peripheral Organs of Mice Using the RT-Biolumicorder

    • Georgia Katsioudi, Alejandro Osorio-Forero, Flore Sinturel, Claudia Hagedorn, Florian Kreppel, Ueli Schibler et al.
    Pages 217-242

  17. Isolation and Sorting of Epidermal Interfollicular Stem Cells for the Study of Circadian Rhythms

    • Paloma Solá, Valentina M. Zinna
    Pages 243-253

  18. Detecting Circadian Rhythms in Human Red Blood Cells by Dielectrophoresis

    • Andrew D. Beale, Fatima H. Labeed, Stephen J. Kitcatt, John S. O’Neill
    Pages 255-264Open Access

  19. Measuring Circadian Neutrophil Infiltration in Tissues by Paired Whole-Mount Tissue Clearing and Flow Cytometry

    • Tommaso Vicanolo, Andres Hidalgo, Jose M. Adrover
    Pages 265-284

  20. In Vivo Imaging of Circadian NET Formation During Lung Injury by Four-Dimensional Intravital Microscopy

    • Alejandra Aroca-Crevillén, Andres Hidalgo, Jose M. Adrover
    Pages 285-300
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Editors and Affiliations

  • Institute for Research in Biomedicine, Barcelona Institute for Science and Tech, Barcelona, Spain

    Guiomar Solanas

  • Hospital del Mar Medical Research Institute (IMIM), Barcelona, Spain

    Patrick -Simon Welz

Bibliographic Information

  • Book Title: Circadian Regulation

  • Book Subtitle: Methods and Protocols

  • Editors: Guiomar Solanas, Patrick -Simon Welz

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2249-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-2248-3Published: 25 May 2022

  • Softcover ISBN: 978-1-0716-2251-3Published: 25 May 2023

  • eBook ISBN: 978-1-0716-2249-0Published: 24 May 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 410

  • Number of Illustrations: 10 b/w illustrations, 74 illustrations in colour

  • Topics: Biochemistry, general, Biological Techniques

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