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Analysis and Control of Proteolysis of Recombinant Proteins in Escherichia coli
Proteolysis is one of the reasons for poor production of recombinant proteins in Escherichia coli. Important properties of E. coli proteases, which...
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Approaches to Avoid Proteolysis During Protein Expression and Purification
All cells contain proteases, which hydrolyze the peptide bonds between amino acids of a protein backbone. Typically, proteases are prevented from...
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Proteolysis-targeting chimeras with reduced off-targets
Proteolysis-targeting chimeras (PROTACs) are molecules that induce proximity between target proteins and E3 ligases triggering target protein...
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Proteolysis and Deficiency of α1-Proteinase Inhibitor in SARS-CoV-2 Infection
The SARS-CoV-2 pandemic had stimulated the emergence of numerous publications on the α 1 -proteinase inhibitor (α 1 -PI, α 1 -antitrypsin), especially when...
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Determination of the effects of proteolysis-based changes by adjunct lactobacilli on the bioactivity (ACE-inhibitory and antioxidant activities) of cheese: a model cheese study
Bioactive properties, proteolysis and microbiology of model cheeses with and without adjunct lactobacilli ( Lactobacillus helveticus , Lactiplantibacillu...
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Innovative UF-white cheese fortified with Ganoderma lucidum extract: antioxidant capacity, proteolysis, microstructure and sensory characteristics
This study was implemented to investigate the effect of Ganoderma lucidum extract (0.0, 0.5, 1.0, 1.5 and 2.0%) on chemical properties, total...
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Huntingtin, the Major Factor in Huntington’s Disease Development. Main Functions and Intracellular Proteolysis
AbstractThis review is devoted to the consideration of pathological intracellular mechanisms characteristic of Huntington’s disease and the central...
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Analysis of complex proteoglycans using serial proteolysis and EThcD provides deep N- and O-glycoproteomic coverage
Proteoglycans are a small but diverse family of proteins that play a wide variety of roles at the cell surface and in the extracellular matrix. In...
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Scaling up the Two-Stage Countercurrent Extraction of Oil and Protein from Green Coffee Beans: Impact of Proteolysis on Extractability, Protein Functionality, and Oil Recovery
Green coffee processing has been hindered by low oil extraction yields from mechanical pressing and the need of using flammable and hazardous...
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Reducing Allergenicity by Proteolysis
Cow’s milk allergy is a health problem of growing concern. However, it is possible to modify the inherent allergenic properties of milk proteins by...
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Coupling of proteolysis-triggered transcription and CRISPR-Cas12a for ultrasensitive protease detection
The efficient signal amplification capacity of several class 2 CRISPR-Cas systems with trans-cleavage activity has exhibited great value in molecular...
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Using a peptide-based mass spectrometry approach to quantitate proteolysis of an intact heterogeneous procollagen substrate by BMP1 for antagonistic antibody screening
Proteases are critical proteins involved in cleaving substrates that may impact biological pathways, cellular processes, or disease progression. In...
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Structural Analysis of SOD1 Fibrils with Mass Spectrometry, Limited Proteolysis, and Atomic Force Microscopy (AFM)
This protocol describes a method to purify SOD1 in Saccharomyces cerevisiae to characterize using ICP-MS and AFM, to agitate and fibrillate for...
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Effect of adjunct starter culture on the quality of reduced fat, white, brined goat cheese: part I. Assessment of chemical composition, proteolysis, lipolysis, texture and sensory attributes
Full fat (4.3%) and two reduced fat (2.5% and 1.4%) milk were used to prepare white brined goat cheeses with the addition of (i) mixed...
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Map** Cell Surface Proteolysis with Plasma Membrane-Targeted Subtiligase
N terminomics methods combine selective isolation of protein N-terminal peptides with mass spectrometry (MS)-based proteomics for global profiling of...
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The effects of curing agents on the proteolysis and lipid oxidation of pastırma produced by the traditional method
In the study, the effects of nitrate and/or nitrite (150 mg/kg KNO 3 , 300 mg/kg KNO 3 , 150 mg/kg NaNO 2 , and 150 mg/kg KNO 3 + 150 mg/kg NaNO 2 ) on...
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Peptidomic profiling of fermented goat milk: considering the fermentation-time dependent proteolysis by Lactobacillus and characterization of novel peptides with Antioxidative activity
In this study, antioxidant activities were evaluated for goat milk fermented with Lactobacillus helveticus MTCC 5463. The fermentation conditions...
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Effect of brine concentration on physico-chemical characteristics, texture, rheological properties and proteolysis level of cheeses produced by an optimized wild cardoon rennet
The main objective of this study was to test the efficiency of a wild cardoon ( Cynara cardunculus L. ) rennet, previously optimized by response...
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Aging of Greater Amberjack Seriola dumerili Meat Promoted by Low-Temperature Heating
Cold aging of fish has gained substantial attention because of improving its taste quality. However, fish aging typically requires approximately...
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Protein Chromatography Methods and Protocols
This third edition expands on the previous editions with updated and new chapters on protein chromatography. Chapters detail protein stability and...