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Research Progress of ARTP Mutagenesis Technology Based on Citespace Visualization Analysis
Atmospheric and room temperature plasma (ARTP) mutagenesis technology has been developed rapidly in recent years because of its simple operation,...
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Mutagenesis and Transgenesis in Plant Breeding
Mutagenesis gives an avenue of possibilities for develo** improved cultivars in crops with limited or no variability in a specific trait....
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Facile Assembly of Combinatorial Mutagenesis Libraries Using Nicking Mutagenesis
Combinatorial mutagenesis is a method where multiple user-defined mutations are encoded at defined positions in a sequence. Combinatorial mutagenic...
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Inverse PCR for Site-Directed Mutagenesis
Inverse PCR is a powerful tool for the rapid introduction of desired mutations at desired positions in a circular double-stranded DNA sequence. In...
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Enhanced Production of Sisomicin in Micromonospora inyoensis by Protoplast Mutagenesis and Fermentation Optimization
Sisomicin is a broad-spectrum aminoglycoside antibiotic and is the precursor of netilmicin and plazomicin. However, the fermentation level of...
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Design and Production of a Novel Anti-PD-1 Nanobody by CDR Grafting and Site-Directed Mutagenesis Approach
Programmed cell death protein-1 (PD-1) is a membrane protein expressed on the surface of activated T-cells, B-cells, natural killer cells, dendritic...
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Domain Shuffling and Site-Saturation Mutagenesis for the Enhanced Inhibitory Potential of Amaranthaceae α-Amylase Inhibitors
Amaranthaceae α-amylase inhibitors (AAIs) are knottin-type proteins with selective inhibitory potential against coleopteran α-amylases. Their small...
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A simplified Gibson assembly method for site directed mutagenesis by re-use of standard, and entirely complementary, mutagenesis primers
BackgroundSite-directed mutagenesis (SDM) is a key method in molecular biology; allowing to modify DNA sequences at single base pair resolution....
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Enhancing (2S)-naringenin production in Saccharomyces cerevisiae by high-throughput screening method based on ARTP mutagenesis
( 2S )-Naringenin, a dihydro-flavonoid, serves as a crucial precursor for flavonoid synthesis due to its extensive medicinal values and physiological...
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Enhancement of cyanobacterial PHB production using random chemical mutagenesis with detection through FACS
Poly-hydroxy-butyrate (PHB) bioplastic resin can be made directly from atmospheric CO 2 using cyanobacteria. However, higher PHB productivities are...
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Site-Directed Mutagenesis Method Mediated by Cas9
This study presents an in vitro CRISPR/Cas9-mediated mutagenic (ICM) system that allows rapid construction of designed mutants or site-saturation...
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Polar mutagenesis of polycistronic bacterial transcriptional units using Cas12a
BackgroundFunctionally related genes in bacteria are often organized and transcribed as polycistronic transcriptional units. Examples are the fim ...
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Metabolic analysis of Schizochytrium sp. mutants with high EPA content achieved with ARTP mutagenesis screening
Eicosapentaenoic acid (EPA) belonged to the ω-3 series of polyunsaturated fatty acids and had physiological functions lipid as regulating blood lipid...
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Combination of genetic engineering and random mutagenesis for improving production of raw-starch-degrading enzymes in Penicillium oxalicum
BackgroundRaw starch-degrading enzyme (RSDE) is applied in biorefining of starch to produce biofuels efficiently and economically. At present, RSDE...
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Computational saturation mutagenesis to explore the effect of pathogenic mutations on extra-cellular domains of TREM2 associated with Alzheimer’s and Nasu-Hakola disease
ContextThe specialised family of triggering receptors expressed on myeloid cells (TREMs) plays a pivotal role in causing neurodegenerative disorders...
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Rapid Cloning of Random Mutagenesis Libraries Using PTO-QuickStep
PTO-QuickStep is a quick and easy molecular cloning technique that allows seamless point integration of a DNA fragment, encoding either a tag or a...
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A new halotolerant xylanase from Aspergillus clavatus expressed in Escherichia coli with catalytic efficiency improved by site-directed mutagenesis
Daily agro-industrial waste, primarily cellulose, lignin, and hemicellulose, poses a significant environmental challenge. Harnessing...
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Crop Improvement for Sustainable Food and Nutritional Security: Applications of Mutagenesis and In Vitro Techniques
Agricultural production is the main livelihood-sustaining venture for millions of people across the world, particularly sub-Saharan Africa....
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A stepwise mutagenesis approach using histidine and acidic amino acid to engineer highly pH-dependent protein switches
Antibody-based drugs can be highly toxic, because they target normal tissue as well as tumor tissue. The pH value of the extracellular...
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RETRACTED ARTICLE: Mutagenesis combined with fermentation optimization to enhance gibberellic acid GA3 yield in Fusarium fujikuroi
Gibberellic acid (GA3) is a plant growth hormone that plays an important role in the production of crops, fruits, and vegetables with a wide market...
