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Multiplex Lysosomal Enzyme Activity Assay on Dried Blood Spots Using Tandem Mass Spectrometry
Deficiencies of the enzymes in lysosomes result in the accumulation of undegraded materials and subsequently cellular dysfunction. Early...
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CTSB Nuclear Translocation Facilitates DNA Damage and Lysosomal Stress to Promote Retinoblastoma Cell Death
Retinoblastoma (RB) is a pernicious tumor originating from photoreceptor precursor cells that often endangers the lives of children. The purpose of...
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Impact of Lysosomal Fraction on Melanin Synthesis Inhibition in a Zebrafish Melanogenesis Phenotype Model
To develop whitening cosmetic materials, we conducted a study on lysosomes that decisively contribute to the decomposition of melanin during...
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Diagnostics of lysosomal storage diseases by mass spectrometry: a review
Lysosomal storage disorders (LSD) are a group of over 70 rare inherited metabolic disorders that are caused mostly by the deficiency of specific...
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Quantifying lysosomal glycosidase activity within cells using bis-acetal substrates
Understanding the function and regulation of enzymes within their physiologically relevant milieu requires quality tools that report on their...
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Immobilized enzyme cascade for targeted glycosylation
Glycosylation is a critical post-translational protein modification that affects folding, half-life and functionality. Glycosylation is a...
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Role of Lysosomal Cholesterol in Regulating PI(4,5)P2-Dependent Ion Channel Function
Lysosomes are central regulators of cellular growth and signaling. Once considered the acidic garbage can of the cell, their ever-expanding...
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Lysosomal and Other Indigenous Non-plasmin Proteases in Bovine Milk
Bovine milk contains a range of indigenous proteolytic enzymes and enzyme systems. The major bovine proteolytic enzyme in milk is plasmin (Chap. 2),...
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A Review of Background and Application of ATPSs in Protein and Enzyme Extraction
This review article provides an overview of the fundamental background and application of aqueous two-phase systems (ATPSs) in protein and enzyme...
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A lava-inspired proteolytic enzyme therapy on cancer with a PEG-based hydrogel enhances tumor distribution and penetration of liposomes
The enhanced permeability and retention (EPR) effect has become the guiding principle for nanomedicine against cancer for a long time. However,...
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Lysosomal escaped protein nanocarriers for nuclear-targeted siRNA delivery
In the process of drug carrier design, lysosome degradation in cells is often neglected, which makes a considerable number of drugs not play a role....
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Removal of Trans-2-nonenal Using Hen Egg White Lysosomal-Related Enzymes
2-Nonenal is a long-chain aliphatic aldehyde containing nine carbons and an unsaturated bond. 2-Nonenal is the primary cause of odor associated with...
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Synthesis and Characterization of Dual-Sensitive PAMAM Derivatives Conjugated with Enzyme Cleavable Peptides as Gene Carriers
Nonviral vectors have the potential for gene delivery comparable to viral vectors given their productive and economic advantages. However, the low...
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Mitochondria–lysosome crosstalk in GBA1-associated Parkinson’s disease
Organelle crosstalk is significant in regulating their respective functions and subsequent cell fate. Mitochondria and lysosomes are amongst the...
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Effect of Lysosomes on Extending Vase Life of Cut Flowers
In this study, we were investigated the effect of lysosomal extracts (named as lysosomal enzymes) on extending the vase life of cut flowers. The...
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Autophagy: a necessary evil in cancer and inflammation
Autophagy, a highly regulated cellular process, assumes a dual role in the context of cancer. On the one hand, it functions as a crucial homeostatic...
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The interrupted effect of autophagic flux and lysosomal function induced by graphene oxide in p62-dependent apoptosis of F98 cells
BackgroundGraphene oxide (GO) nanoparticles (NPs) have been widely applied in various fields, especially in biomedical applications. Extensive...
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Comprehensive dry eye therapy: overcoming ocular surface barrier and combating inflammation, oxidation, and mitochondrial damage
BackgroundDry Eye Disease (DED) is a prevalent multifactorial ocular disease characterized by a vicious cycle of inflammation, oxidative stress, and...
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Microbial Hyaluronidase: Its Production, Purification and Applications
Hyaluronidase (hyase) is an enzyme from glycosidase family that degrades hyaluronic acid (HA) and other associated glycosaminoglycans. It precisely...
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Canonical Inflammasomes
The innate immune response represents the first line of host defense, and it is able to detect pathogen- and damage-associated molecular patterns...