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Microbial Hyaluronidase: Its Production, Purification and Applications

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Abstract

Hyaluronidase (hyase) is an enzyme from glycosidase family that degrades hyaluronic acid (HA) and other associated glycosaminoglycans. It precisely cleaves the β,1–4 glycosidic linkages in hyaluronic acid substrate. Generally pathogenic Gram-positive bacteria produce hyases; where enzyme serves as virulence factor that facilitate the spreading of bacteria in host tissues by degradation of hyaluronic acid present in connective tissues. The optimization of nutritional and physical parameters for hyaluronidase synthesis carries high importance in enzyme yield improvement. The purification of extracellular microbial hyase from a protein mixture could be achieved by a series of purification steps like solvent and salt precipitation, membrane technology and various chromatographic methods. The present chapter summarizes hyase production, purification and its applications. The interest in hyaluronidase is increasing due to its various applications.

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Acknowledgements

All authors acknowledge the kind help of R. C. Patel Arts, Commerce and Science College, Shirpur, MS, India, for providing infrastructural facilities. Corresponding author (BLC) is grateful to FIST grant of DST, New Delhi, for improving research facilities provided to the School of Life Sciences of KBCNMU.

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Authors and Affiliations

  1. Department of Microbiology and Biotechnology, R. C. Patel Arts, Commerce and Science College, Shirpur, India

    Sandip P. Patil & Leena P. Shirsath

  2. Department of Microbiology, School of Life Sciences, Kavayitri Bahinabai Chaudhari North Maharashtra University, Jalgaon, India

    Kiran S. Dalal & Bhushan L. Chaudhari

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  1. Sandip P. Patil
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  2. Kiran S. Dalal
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  3. Leena P. Shirsath
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  4. Bhushan L. Chaudhari
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Correspondence to Bhushan L. Chaudhari .

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  1. Bioprocess and Bioenergy Laboratory, Department of Microbiology, Central University of Rajasthan, Kishangarh, Rajasthan, India

    Pradeep Verma

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Patil, S.P., Dalal, K.S., Shirsath, L.P., Chaudhari, B.L. (2023). Microbial Hyaluronidase: Its Production, Purification and Applications. In: Verma, P. (eds) Industrial Microbiology and Biotechnology. Springer, Singapore. https://doi.org/10.1007/978-981-99-2816-3_16

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