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Exclusion Chromatography
Exclusion chromatography separates molecules on the basis of size. It thus justifies why it is also known as Size Exclusion Chromatography (SEC).
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Fluorescence-Detection Size-Exclusion Chromatography-Based Thermostability Assay for Membrane Proteins
Green fluorescent proteins (GFPs) have lightened up almost every aspect of biological research including protein sciences. In the field of membrane...
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Size-Exclusion Chromatography: A Twenty-First Century Perspective
Now in its sixth decade, size-exclusion chromatography (SEC) remains the premier method by which to determine the molar mass averages and...
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Physical origin of the peak tailing of monoclonal antibodies in size-exclusion chromatography using bio-compatible systems and columns
AbstractThe analysis of mixtures containing monoclonal antibody (mAb) (approximately 150 kDa molecular weight) and sub-unit impurities (approximately...
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The Use of Size-Exclusion Chromatography and Laser Desorption/Ionization for Studying Silver Nanoparticles Synthesized in Reverse Micelles
AbstractMass spectrometry with matrix/surface-assisted laser desorption/ionization has been employed to confirm the formation of nanoparticles as a...
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Size Exclusion Chromatography Strategies and MASH Explorer for Large Proteoform Characterization
Top-down mass spectrometry (MS)-based analysis of larger proteoforms (>50 kDa) is typically challenging due to an exponential decay in the...
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Studies of extractable organohalogens in farmed Atlantic salmon muscle and skin by neutron activation in conjunction with size exclusion and solid phase extraction chromatography
An instrumental neutron activation analysis method in conjunction with Compton suppression spectrometry was developed for the simultaneous...
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Gel Chromatography
It is also known as molecular chromatography or size exclusion chromatography. In this type of chromatography, molecular sieve is used as a...
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Methods to Measure NLR Oligomerization I: Size Exclusion Chromatography, Co-immunoprecipitation, and Cross-Linking
Protein oligomerization is a common principle of regulating cellular responses. Oligomerization of NLRs is essential for the formation of NLR...
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Modeling study of long-term stability of the monoclonal antibody infliximab and biosimilars using liquid-chromatography–tandem mass spectrometry and size-exclusion chromatography–multi-angle light scattering
Monoclonal antibodies (mAbs) represent a dynamic class of biopharmaceutical products, as evidenced by an increasing number of market authorizations...
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Basic Principles of Chromatography
Chromatography has many applications for analysis of various foods components. It differs from other methods of separation in that a wide variety of...
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High-Performance Liquid Chromatography
High-performance liquid chromatography (HPLC) is a chromatographic technique of great versatility and analytical power that can be applied to any...
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Chromatography
The term chromatography was coined by a Russian scientist Tswett (1906), who reported the separation of different coloured constituents of leaves by...
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Chromatography
Chromatography is among the most promising methods of separation. Since analytical chemistry primarily involves identification and separation of...
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Gas Chromatography
Gas chromatography (GC) has been used for the determination of a wide range of food components, but it is ideally suited for the analysis of...
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Monitoring the Cd2+ release from Cd-containing quantum dots in simulated body fluids by size exclusion chromatography coupled with ICP-MS
Quantification of Cd 2+ release from Cd-containing quantum dots (QDs) is of fundamental importance to elucidate its toxicity to organisms, but remains...
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Estimation of Degree of Hydrolysis of Protein Hydrolysates by Size Exclusion Chromatography
Estimating the degree of hydrolysis (DH) of commercial protein hydrolysates from their molecular weight (MW) median values generated by high...
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Analysis of Oligomeric and Glycosylated Proteins by Size-Exclusion Chromatography Coupled with Multiangle Light Scattering
Analytical size-exclusion chromatography (SEC) is a powerful technique that separates proteins based on their hydrodynamic radii. This approach can...
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Recent Advances in Liquid Chromatography Analysis of Synthetic Polymers
Synthetic polymers are rarely homogeneous chemical species but have multivariate distributions in molecular weight, chemical composition, chain...