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Genome-wide identification, characterization and primer designing of simple sequence repeats across Leguminosae family
Legumes are important clade of commercially important family Leguminosae that mainly include medicinal, flowering and edible plants. Although the...
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Implications of genome simple sequence repeats signature in 98 Polyomaviridae species
The analysis of simple sequence repeats (SSRs) in 98 genomes across four genera of the family Polyomaviridae was performed. The genome size ranged...
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Molecular Detection for Identification and Seed Adulteration of Camellia Oils Based on Chloroplast Simple Sequence Repeat Markers
Tea seed oil is a popular cooking oil in Chinese traditional culture, but its high cost makes it a target for adulteration. This study presents a...
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Align-gram: Rethinking the Skip-gram Model for Protein Sequence Analysis
The inception of next generations sequencing technologies have exponentially increased the volume of biological sequence data. Protein sequences,...
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Phage-assisted evolution of compact Cas9 variants targeting a simple NNG PAM
Compact Cas9 nucleases hold great promise for therapeutic applications. Although several compact Cas9 nucleases have been developed, many genomic...
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The Complete Chloroplast Genome Sequence of Staphylea holocarpa (Staphyleaceae)
Staphylea holocarpa (Hemsley 1895) is an ornamental deciduous shrub or tree in the family Staphyleaceae. As the shortage of the wild resources, S....
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Use of 2D FFT and DTW in Protein Sequence Comparison
Protein sequence comparison remains a challenging work for the researchers owing to the computational complexity due to the presence of 20 amino...
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Engineering the N-Terminal Sequence of Glycine max Soybean Formate Dehydrogenase
AbstractNAD(P) + -dependent formate dehydrogenase (FDH, EC 1.2.1.2.) catalyzes the oxidation of formate ion with the coupled reduction of NAD(P) + to...
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Loop-mediated isothermal amplification targeting proteinase inhibitor II terminator sequence: an efficient approach for screening of genetically modified crops
Proteinase inhibitor II ( T-pinII ) has been employed as terminator sequence in 25% of globally approved genetically modified (GM) crops (maize,...
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Analysis of Tandem Repeat Expansions Using Long DNA Reads
Abnormal expansion or shortening of tandem repeats can cause a variety of genetic diseases. The use of long DNA reads has facilitated the analysis of...
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Characterization and development of transcriptome-derived novel EST-SSR markers to assess genetic diversity in Chaetomium globosum
Chaetomium globosum Kunze, an internationally recognized biocontrol fungus. It mycoparasitizes various plant pathogens and produce antifungal...
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DNA-Wrapped CNT Sensor for Small Nucleic Acid Detection: Influence of Short Complementary Sequence
Carbon nanotubes (CNTs) are versatile materials that act as natural fluorescence quenchers and double scaffolds for DNA that can wrap around them...
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A Simple Allelic Exchange Method for Efficient Seamless Knockout of Up to 34-kbp-Long Gene Cassettes in Pseudomonas
Gene knockout is a widely used technique for engineering bacterial genomes, investigating the roles of genes in metabolism, and conferring biological...
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A simple “signal off–on” fluorescence nanoplatform for the label-free quantification of exosome-derived microRNA-21 in lung cancer plasma
A simple nanoplatform based on molybdenum disulfide (MoS 2 ) nanosheets, a fluorescence quencher (signal off), and a hybridization chain reaction (HCR)...
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Genome-wide identification and characterization of microsatellite markers within the Avipoxviruses
Microsatellite markers or Simple Sequence Repeats (SSRs) are gaining importance for molecular characterization of the virus as well as estimation of...
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Comprehensive Analysis of the Complete Chloroplast Genome of Cinnamomum daphnoides (Lauraceae), An Endangered Island Endemic Plant
Cinnamomum daphnoides (Siebold & Zucc 1846) is a rare and endangered island species with a unique Sino-Japanese distribution pattern. However,...
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Production and Purification of Plasmodium Circumsporozoite Protein in Lactococcus lactis
Malaria is a vector-borne disease caused by Plasmodium parasites of which Plasmodium falciparum contributed to an estimated 247 million cases...
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Insights into genome plasticity and gene regulation in Orientia tsutsugamushi through genome-wide mining of microsatellite markers
Microsatellite markers are being used for molecular identification and characterization as well as estimation of evolution patterns due to their...
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Conservation Strategies for Aquilaria sinensis: Insights from DNA Barcoding and ISSR Markers
The evergreen tree species Aquilaria sinensis holds significant economic importance due to its specific medicinal values and increasing market...
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Pipeline for develo** polymorphic microsatellites in species without reference genomes
Microsatellites, also known as simple sequence repeats (SSRs), are the preferred type of marker for many genetic applications. In conjunction with...
