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Recombinant Protein Expression in Mammalian Cells Methods and Protocols
This fully updated volume explores notable developments in the field of mammalian cell-based recombinant protein production. Beginning with methods...
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Stress Induced by Recombinant Protein Production in Escherichia coli
Strong production of recombinant proteins interferes with cellular processes in many ways. Drainage of precursors and energy urges the cell to...
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Recombinant Protein Production from Stable CHO Cell Pools
The continuous improvement of expression platforms is necessary to respond to the increasing demand for recombinant proteins that are required to...
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The Use of Baculovirus-Mediated Gene Expression in Mammalian Cells for Recombinant Protein Production
Baculovirus-mediated gene expression in mammalian cells, BacMam, is a useful alternative to transient transfection for recombinant protein production...
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Understanding exopolysaccharide byproduct formation in Komagataella phaffii fermentation processes for recombinant protein production
BackgroundKomagataella phaffii ( Pichia pastoris ) has emerged as a common and robust biotechnological platform organism, to produce recombinant...
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Unlocking Nature’s Toolbox: glutamate-inducible recombinant protein production from the Komagatella phaffii PEPCK promoter
BackgroundKomagataella phaffii (a.k.a. Pichia pastoris) harbors a glutamate utilization pathway in which synthesis of glutamate dehydrogenase 2 and...
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Bench-Scale Stirred-Tank Bioreactor for Recombinant Protein Production in Chinese Hamster Ovary (CHO) Cells in Suspension
Most pharmaceutical biotechnology companies use stirred-tank bioreactors (STR) for recombinant protein manufacturing. These bioreactors are used at a...
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Thermoinducible E. coli for Recombinant Protein Production in Inclusion Bodies
The temperature-inducible λpL/pR-cI857 expression system has been widely used to produce recombinant proteins (RPs), especially when it is necessary...
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Protein production dynamics and physiological adaptation of recombinant Komagataella phaffii at near-zero growth rates
BackgroundSpecific productivity (q P ) in yeast correlates with growth, typically peaking at intermediate or maximum specific growth rates (μ)....
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Inducer-free recombinant protein production in Trichoderma reesei: secretory production of endogenous enzymes and heterologous nanobodies using glucose as the sole carbon source
BackgroundThe filamentous fungus Trichoderma reesei has been used as a host organism for the production of lignocellulosic biomass-degrading...
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CRISPR Deletion of miR-27 Impacts Recombinant Protein Production in CHO Cells
MicroRNAs represent an interesting group of regulatory molecules with the unique ability of a single miRNA able to regulate the expression of...
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Cheese-whey permeate improves the fitness of Escherichia coli cells during recombinant protein production
BackgroundEscherichia coli cells are the most frequently used hosts in recombinant protein production processes and mainly require molecules such as...
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Roles of Heat-Shock Chaperones in the Production of Recombinant Proteins in Escherichia coli
Escherichia coli is a versatile organism for the production of recombinant proteins. Often, however, the recombinant protein does not reach its...
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Transgenic Birds for the Production of Recombinant Proteins
Transgenic birds were expected to be an excellent transgenic bioreactor for the production of recombinant pharmaceutical proteins. However, the only...
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Modifications of the 5’ region of the CASPONTM tag’s mRNA further enhance soluble recombinant protein production in Escherichia coli
BackgroundEscherichia coli is one of the most commonly used host organisms for the production of biopharmaceuticals, as it allows for cost-efficient...
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Biotechnological Production of Melanins with Recombinant Microorganisms
Most organisms can synthesize a variety of natural polymers called melanins. These substances serve protective roles against physical and chemical...
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Apoplastic Production of Recombinant AntiVEGF Protein Using Plant-Virus Transient Expression Vector
Targeting of vascular endothelial growth factor (VEGF) using AntiVEGF can be a promising approach for angiogenesis inhibition and cancer therapy. In...
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Protein Chromatography Methods and Protocols
This third edition expands on the previous editions with updated and new chapters on protein chromatography. Chapters detail protein stability and...
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Production and secretion of recombinant spider silk in Bacillus megaterium
BackgroundSilk proteins have emerged as versatile biomaterials with unique chemical and physical properties, making them appealing for various...
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Exploring Functionality Gain for (Recombinant) β-Lactoglobulin Through Production and Processing Interventions
Interventions in the upstream production and further processing of recombinant food proteins affect its properties when used for food application....
