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Protocols for Building and Producing High Diversity Peptide Phage Display Libraries
Phage display is an important technology to study protein-protein interaction and protein evolution, with applications in basic science and applied...
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Phage Display as a Medium for Target Therapy Based Drug Discovery, Review and Update
Phage libraries are now amongst the most prominent approaches for the identification of high-affinity antibodies/peptides from billions of displayed...
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Generation of a Naïve Human scFv Phage Display Library and Panning Selection
Phage display antibody libraries have been successfully used as the essential tool to produce monoclonal antibodiesMonoclonal antibodies (mAB)...
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Ligand Selection for Affinity Chromatography Using Phage Display
Phage display coupled with in vitro affinity selection to mimic evolutionary principles has propelled the discovery of specific binding peptides and...
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A Simple Whole-Plasmid PCR Method to Construct High-Diversity Synthetic Phage Display Libraries
Phage display technology utilises peptide and antibody libraries with very high diversities to select ligands with specific binding properties. The...
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Preparation of Bioconjugates of Chimeric M13 Phage and Gold Nanorods
Phage-nanomaterial conjugates are functional bio-nanofibers with various applications. While phage display can select for phages with desired...
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Highly potent multivalent VHH antibodies against Chikungunya isolated from an alpaca naïve phage display library
BackgroundChikungunya virus (CHIKV) is a re-emerged mosquito-borne alphavirus that can cause musculoskeletal diseases, imposing a substantial threat...
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Development of a human phage display-derived anti-PD-1 scFv antibody: an attractive tool for immune checkpoint therapy
BackgroundThe PD-1 checkpoint pathway plays a major role in tumor immune evasion and the development of the tumor microenvironment. Clinical studies...
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Phage-based delivery systems: engineering, applications, and challenges in nanomedicines
Bacteriophages (phages) represent a unique category of viruses with a remarkable ability to selectively infect host bacteria, characterized by their...
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A highly sensitive electrochemiluminescence method for abrin detection by a portable biosensor based on a screen-printed electrode with a phage display affibody as specific labeled probe
Abrin is a highly toxic ribosome-inactivating protein, which could be used as a biological warfare agent and terrorist weapon, and thus needs to be...
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M13 phage: a versatile building block for a highly specific analysis platform
Viruses are changing the biosensing and biomedicine landscape due to their multivalency, orthogonal reactivities, and responsiveness to genetic...
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Breaking barriers in electrochemical biosensing using bioinspired peptide and phage probes
Electrochemical biosensing continues to advance tirelessly, overcoming barriers that have kept it from leaving research laboratories for many years....
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Phage display-derived immunorecognition elements LSPR nanobiosensor for peptide hormone glycine-extended gastrin 17 detection
The current study intended to evaluate two types of biorecognition element (BRE), namely recombinant antibody fragments and M13...
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Single-Cell Proteomics by Barcoded Phage-Displayed Screening via an Integrated Microfluidic Chip
Recent advancements in the profiling of proteomes at the single-cell level necessitate the development of quantitative and versatile platforms,...
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Selection of Cancer Stem Cell–Targeting Agents Using Bacteriophage Display
There is a growing need to develop tumor targeting agents for aggressive cancers. Aggressive cancers frequently relapse and are resistant to various...
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Visualization and quantitative detection of foodborne Salmonella Typhimurium by functionalizing cysteamine (CS) stabilized gold nanoparticles (CS-AuNPs@phage)
Background: Salmonella , is one of the most important pathogens causing foodborne illnesses worldwide. The early detection of Salmonella is essential...
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Mobile CRISPR-Cas9 based anti-phage system in E. coli
Escherichia coli is one of the most important microbial cell factories, but infection by bacteriophages in the environment may have a huge impact on...
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Production of Phage Display-Derived Peptide and the Application for Detecting Vibrio parahaemolyticus by Combined PCR Technology
Vibrio parahaemolyticus ( V. parahaemolyticus ) is one of the most common and widely distributed food-borne bacteria. Thus, it is necessary to...
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Cell-Free Display Techniques for Protein Evolution
Cell-free protein synthesis (CFPS) with flexibility and controllability can provide a powerful platform for high-throughput screening of...
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Development of a phage display-mediated immunoassay for the detection of vascular endothelial growth factor
Because of the critical role of vascular endothelial growth factor (VEGF) in angiogenesis and its significantly increased serum levels in early...
