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Preparation of Bioconjugates of Chimeric M13 Phage and Gold Nanorods

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Phage Engineering and Analysis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2793))

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Abstract

Phage-nanomaterial conjugates are functional bio-nanofibers with various applications. While phage display can select for phages with desired genetically encoded functions and properties, nanomaterials can endow the phages with additional features at nanoscale dimensions. Therefore, combining phages with nanotechnology can construct bioconjugates with unique characteristics. One strategy for filamentous phages is to adsorb nanoparticles onto the side wall, composed of pVIII subunits, through electrostatic interactions. However, a noncovalent approach may cause offloading if the environment changes, potentially causing side effects especially for in vivo applications. Therefore, building stable phage-bioconjugates is an important need. We previously reported the construction of chimeric M13 phage conjugated with gold nanorods, named “phanorods,” without weakening the binding affinity to the bacterial host cells. Herein, we give a detailed protocol for preparing the chimeric M13 phage and covalently conjugating gold nanorods to the phage.

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Acknowledgements

This study was supported by the National Natural Science Foundation of China (Grant No. 32201100) (H.P.) and the U.S. National Institutes of Health (DP2GM123457 to IAC).

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Authors and Affiliations

  1. Cellular Signaling Laboratory, International Research Center for Sensory Biology and Technology of MOST, Key Laboratory of Molecular Biophysics of MOE, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, Hubei, China

    Huan Peng

  2. Department of Chemical and Biomolecular Engineering, Department of Chemistry and Biochemistry, University of California, Los Angeles, CA, USA

    Irene A. Chen

Authors
  1. Huan Peng
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  2. Irene A. Chen
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Correspondence to Huan Peng .

Editor information

Editors and Affiliations

  1. Cellular Signaling Laboratory International Research, Center for Sensory Biology and Technology of MOST, Key Laboratory of Molecular Biophysics of MOE, College of Life Science and Technology, Huazhong University of Science and Technology (HUST), Wuhan, Hubei, China

    Huan Peng

  2. College of Life Science and Technology, Huazhong University of Science and Technology (HUST), Wuhan, Hubei, China

    Jianfeng Liu

  3. Department of Chemical and Biomolecular Engineering, Department of Chemistry and Biochemistry, University of California, Los Angeles, CA, USA

    Irene A. Chen

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Peng, H., Chen, I.A. (2024). Preparation of Bioconjugates of Chimeric M13 Phage and Gold Nanorods. In: Peng, H., Liu, J., Chen, I.A. (eds) Phage Engineering and Analysis. Methods in Molecular Biology, vol 2793. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3798-2_9

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  • DOI: https://doi.org/10.1007/978-1-0716-3798-2_9

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-3797-5

  • Online ISBN: 978-1-0716-3798-2

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